LOCUS       Exported                 984 bp ds-DNA     linear   SYN 08-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    p3993 pET ubcH7
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Scheffner M, Huibregtse JM, Howley PM
  TITLE     Identification of a human ubiquitin-conjugating enzyme that mediates
            the E6-AP-dependent ubiquitination of p53.
  JOURNAL   Proc Natl Acad Sci U S A 1994 Sep 13;91(19):8797-801.
  PUBMED    8090726
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 8, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(541..559)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      555..602
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gtttacttta gaaggagata tacatatggc ggccagcagg aggctgatga aggagcttga
       61 agaaatccgc aaatgtggga tgaaaaactt ccgtaacatc caggttgatg aagctaattt
      121 attgacttgg caagggctta ttgttcctga caaccctcca tatgataagg gagccttcag
      181 aatcgaaatc aactttccag cagagtaccc attcaaacca ccgaagatca catttaaaac
      241 aaagatctat cacccaaaca tcgacgaaaa ggggcaggtc tgtctgccag taattagtgc
      301 cgaaaactgg aagccagcaa ccaaaaccga ccaagtaatc cagtccctca tagcactggt
      361 gaatgacccc cagcctgagc acccgcttcg ggctgaccta gctgaagaat actctaagga
      421 ccgtaaaaaa ttctgtaaga atgctgaaga gtttacaaag aaatatgggg aaaagcgacc
      481 tgtggactaa ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
      541 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
      601 tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg
      661 tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg
      721 gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc
      781 acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt
      841 accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg
      901 agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttta ctgtgataaa
      961 ctaccgcatt aaagcttatc gatg
//
