LOCUS Exported 2568 bp ds-DNA linear SYN 02-JUN-2021 DEFINITION P. falciparum plasmid for expression of both diCre fragments from single casette using skip peptide. ACCESSION . VERSION . KEYWORDS pSkipFlox SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2568) AUTHORS Birnbaum J, Flemming S, Reichard N, Soares AB, Mesen-Ramirez P, Jonscher E, Bergmann B, Spielmann T TITLE A genetic system to study Plasmodium falciparum protein function. JOURNAL Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4223. PUBMED 28288121 REFERENCE 2 (bases 1 to 2568) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 2, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2568 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 243..263 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 1440..1493 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 1500..1520 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 1527..1847 /codon_start=1 /product="human FK506-binding protein FKBP12" /label=FKBP /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL KLE" ORIGIN 1 atatatatat atatatttat atatacatgt atgtttttta aaaggttaaa taattctata 61 gattattttc attgtcttcc acatatatga cataaatatt ttaaaatcga cattccgata 121 tattatattt ttagactata atatccgtta ataataaata cacgcagtca tattatttat 181 tatacattca tttattattt tgtttttttt aatttcttac atataactcg agcaaaatgg 241 caccaaagaa aaagagaaaa gtaagtagaa tattatggca tgaaatgtgg catgaaggat 301 tagaagaagc aagtagatta tattttggag aaagaaatgt aaaaggaatg tttgaagtat 361 tagaaccatt acatgcaatg atggaaagag gaccacaaac attaaaagaa acaagtttta 421 atcaagcata tggaagagat ttaatggaag cacaagaatg gtgtagaaaa tatatgaaaa 481 gtggaaatgt aaaagattta ttacaagcat gggatttata ttatcatgta tttagaagaa 541 taagtaaagc tagcccttca aaccctggtg cttcaaacgg ttcaaataga aaatggtttc 601 cagcagaacc agaagatgta agagattatt tattatattt acaagcaaga ggattagcag 661 taaaaacaat acaacaacat ttaggacaat taaatatgtt acatagaaga agtggattac 721 caagaccaag tgatagtaat gcagtaagtt tagtaatgag aagaataaga aaagaaaatg 781 tagatgcagg agaaagagca aaacaagcat tagcatttga aagaacagat tttgatcaag 841 taagaagttt aatggaaaat agtgatagat gtcaagatat aagaaattta gcatttttag 901 gaatagcata taatacatta ttaagaatag cagaaatagc aagaataaga gtaaaagata 961 taagtagaac agatggagga agaatgttaa tacatatagg aagaacaaaa acattagtaa 1021 gtacagcagg agtagaaaaa gcattaagtt taggagtaac aaaattagta gaaagatgga 1081 taagtgtaag tggagtagca gatgatccaa ataattattt attttgtaga gtaagaaaaa 1141 atggagtagc agcaccaagt gcaacaagtc aattaagtac aagagcatta gaaggaatat 1201 ttgaagcaac acatagatta atatatggag caaaagatga tagtggacaa agatatttag 1261 catggagtgg acatagtgca agagtaggag cagcaagaga tatggcaaga gcaggagtaa 1321 gtataccaga aataatgcaa gcaggaggat ggacaaatgt aaatatagta atgaattata 1381 taagaaattt agatagtgaa acaggagcaa tggtaagatt attagaagat ggagatggag 1441 aaggaagagg aagtttatta acatgtggag atgtagaaga aaatccagga ccaatggctc 1501 ctaagaaaaa aagaaaggta agtagaggag tacaagtaga aacaataagt ccaggagatg 1561 gaagaacatt tccaaaaaga ggacaaacat gtgtagtaca ttatacagga atgttagaag 1621 atggaaaaaa atttgatagt agtagagata gaaataaacc atttaaattt atgttaggaa 1681 aacaagaagt aataagagga tgggaagaag gagtagcaca aatgagtgta ggacaaagag 1741 caaaattaac aataagtcca gattatgcat atggagcaac aggacatcca ggaataatac 1801 caccacatgc aacattagta tttgatgtag aattattaaa attagaagca agtccaagta 1861 atccaggagc aagtaatgga tccacaagtg atgaagtaag aaaaaattta atggatatgt 1921 ttagagatag acaagcattt agtgaacata catggaaaat gttattaagt gtatgtagaa 1981 gttgggcagc atggtgtaaa ttaaattaac ccgggtcgag ggatatggca gcttaatgtt 2041 cgtttttctt atttatatat ttataccaat tgattgtatt tataactgta aaaatgtgta 2101 tgttgtgtgc atattttttt ttgtgcatgc acatgcatgt aaatagctaa aattatgaac 2161 attttatttt ttgttcagaa aaaaaaaact ttacacacat aaaatggcta gtatgaatag 2221 ccatatttta tataaattaa atcctatgaa tttatgacca tattaaaaat ttagatattt 2281 atggaacata atatgtttga aacaataaga caaaattatt attattatta ttatttttac 2341 tgttataatt atgttgtctc ttcaatgatt cataaatagt tggacttgat ttttaaaatg 2401 tttataatat gattagcata gttaaataaa aaaagttgaa aaattaaaaa aaaacatata 2461 aacacaaatg atgttttttc cttcaatttc gatatcaatt tatagaaaca aaatatatac 2521 ttgtataatt ttattttttt atataaatca ttacatatat aattatac //