LOCUS       Exported                3592 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Constitutively expresses RpaR and inducibly expresses LasI.
ACCESSION   .
VERSION     .
KEYWORDS    Bsrs103-RpaR-LasI
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Scott SR, Hasty J
  TITLE     Quorum Sensing Communication Modules for Microbial Consortia.
  JOURNAL   ACS Synth Biol. 2016 Sep 16;5(9):969-77. doi: 
            10.1021/acssynbio.5b00286. Epub 2016 May 19.
  PUBMED    27172092
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      33..127
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(158..952)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(269..288)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     879..898
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1131..1149
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     RBS             2024..2035
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2645..2677
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag
                     /note="wild-type LAA variant"
                     /translation="AANDENYALVA"
     terminator      2698..2784
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(2949..3537)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3029..3048)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttaagggat tttggtcatg actagtgctt ggattctcac caataaaaaa cgcccggcgg
       61 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
      121 aggagtccaa gcgagctctc gaaccccaga gtcccgctca gaagaactcg tcaagaaggc
      181 gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt
      241 cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat
      301 agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca
      361 ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca
      421 tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca
      481 gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt
      541 tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat
      601 cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg
      661 gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg
      721 cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tgcagttcat
      781 tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc
      841 ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc
      901 tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg
      961 atcctcatcc tgtctcttga tcagatcttg atcccctgcg ccatcagatc cttggcggca
     1021 agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc gccccagctg
     1081 gcaattccga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     1141 tcacgaggcc ctttcgtctt cacccctaga cctagccgtc ttcacctcga gtcacaaacg
     1201 gatcaatccg agcgtcaatg ccttggcaac cgccgcggtg cggttggcgg cgtcgagctt
     1261 gcgggcggct tcgatcagat ggaatttcac cgtgcgttcg gtgatgcaga ggattaccga
     1321 gatttcccag gcggtcttgc cctgcgctgc ccattgcagg atctcgcact cgcgcggcgt
     1381 gagccggttg cgccggatcg gctttggccg gctgagtgcg cgcaggcgac tatgcgcgta
     1441 gatgctgacc agctgcatcg cgccgcgcgc ggccgggctg aggtccggat ccttgccggc
     1501 catgctgatc gcggcgctac cgtcgtcgta gtgcagcgga atgcagtaac cctcgaccag
     1561 tccgaattcc gccgcccggg tcatgacccg gtgggcggcc ggatcacggt cgcggtcgta
     1621 gggtgcatcg gaccatacga aaggatgaac cgtggtagcg ccgtggcgcg gcaccggatc
     1681 gaccgcgctg aagttttcgc tgacatacag atcgaaccag tctcgcggcc agccattggc
     1741 cagcgtcagc tccggtagtc cggcattgcg cgacggcagg ccggccatga tgtaggcggt
     1801 aaatccgcag ctcgcgatca gcgattcgaa ccggctgatc agcgccggcg cctcgagccg
     1861 ttcgacggaa tcgacgaatt ccagcgcacg ccgtccccaa agctgatctt cgccgacgat
     1921 catactattg tatcgctggg aatacaatta cttaacataa gcacctgtag gatcgtacag
     1981 gtttacgcaa gaaaatggtt tgttatagtc gaatgaattc attaaagagg agaaaggtac
     2041 catgatcgtt cagatcggtc gtcgtgaaga gttcgacaaa aaactgctgg gtgaaatgca
     2101 caaactgcgt gctcaggttt tcaaagaacg taaaggttgg gacgtttccg ttatcgacga
     2161 aatggaaatc gacggttacg acgctctgtc cccgtactac atgctgatcc aggaagacac
     2221 cccggaagct caggttttcg gttgctggcg tatcctggac accaccggtc cgtacatgct
     2281 gaaaaacacc ttcccggaac tgctgcacgg taaagaagct ccgtgctccc cgcacatctg
     2341 ggaactgtcc cttttcgcta tcaactccgg tcagaaaggt tccctgggtt tctccgactg
     2401 caccctggaa gctatgcgtg ctctggctcg ttactccttg cagaacgaca tccagaccct
     2461 ggttaccgtt accaccgttg gtgttgaaaa aatgatgatc cgtgctggtc tggacgtttc
     2521 ccgtttcggt ccgcacctga aaatcggtat cgaacgtgct gttgctctgc gtatcgaact
     2581 gaacgctaaa acccagatcg ctctgtacgg tggtgttctg gttgaacagc gtctggctgt
     2641 ttccgctgct aacgacgaaa actacgctct ggttgcttag acgcgtgcta gaggcatcaa
     2701 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     2761 aacgctctcc tgagtaggac aaatccgccg ccctagacct agggcgttcg gctgcggcga
     2821 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     2881 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     2941 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3001 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3061 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3121 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3181 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3241 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3301 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3361 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     3421 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3481 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3541 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc ac
//
