LOCUS       Exported                9720 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  pHis-par2-UbHs/Ubc5Sc/ Uba1Ta.
ACCESSION   .
VERSION     .
KEYWORDS    pGEN6-Ubc5 Sc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9720)
  AUTHORS   Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, 
            Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T,
            Katina C, Pilzer I, Ben-Aroya S, Prag G
  TITLE     A bacterial genetic selection system for ubiquitylation cascade 
            discovery.
  JOURNAL   Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 
            2016 Oct 3.
  PUBMED    27694912
REFERENCE   2  (bases 1 to 9720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9720
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(99..118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     309..330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(857..1672)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1580..1599
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1664..1683
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1994..2582
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2483..2502
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2736..2753
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2768..2910
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2836..2855)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2996..3018
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3012..3203)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(4012..5094)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     5056..5075
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(5095..5172)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     5378..5397
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     5481..5500
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5481..5499
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5500..5524
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5539..5561
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5581..5598
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5620..5640
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5653..5880
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             8675..8686
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             9564..9581
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9634..9652)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9648..9695
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tggatccgtc gacctgcagg gggggggggg cgctgaggtc tgcctcgtga agaaggtgtt
      601 gctgactcat accaggcctg aatcgcccca tcatccagcc agaaagtgag ggagccacgg
      661 ttgatgagag ctttgttgta ggtggaccag ttggtgattt tgaacttttg ctttgccacg
      721 gaacggtctg cgttgtcggg aagatgcgtg atctgatcct tcaactcagc aaaagttcga
      781 tttattcaac aaagccgccg tcccgtcaag tcagcgtaat gctctgccag tgttacaacc
      841 aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca
      901 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact
      961 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc
     1021 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat
     1081 caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt tctttccaga
     1141 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt
     1201 tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat
     1261 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt
     1321 cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg
     1381 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa
     1441 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt
     1501 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg
     1561 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt
     1621 tggaatttaa tcgcggcctc gagcaagacg tttcccgttg aatatggctc ataacacccc
     1681 ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt
     1741 gtgcaatgta acatcagaga ttttgagaca caacgtggct ttcccccccc cccctgcagg
     1801 tcgacggatc caagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     1861 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     1921 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1981 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2041 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2101 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     2161 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2221 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2281 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2341 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2401 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2461 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2521 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2581 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2641 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     2701 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     2761 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     2821 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
     2881 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     2941 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     3001 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa
     3061 agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc
     3121 tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg
     3181 gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg
     3241 gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat
     3301 gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag
     3361 agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag
     3421 ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc
     3481 cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc
     3541 gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc
     3601 taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg
     3661 cgcacccgtg gggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg
     3721 gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac
     3781 aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct
     3841 gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata
     3901 gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt
     3961 cgagatcccg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc
     4021 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     4081 agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa
     4141 cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt
     4201 ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct
     4261 gtcttcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc
     4321 ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg
     4381 aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc
     4441 gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc
     4501 cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg
     4561 acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat
     4621 actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc
     4681 agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac
     4741 gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac
     4801 catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat
     4861 ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt
     4921 gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc
     4981 cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt
     5041 ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac
     5101 caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca
     5161 ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaagcagc
     5221 ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga
     5281 tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc
     5341 tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc
     5401 cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg
     5461 agatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa
     5521 ttcccctcta gaaataattt tgtttaactt taagaaggag atatacatat gtcgtactac
     5581 catcaccatc accatcacga ttacgatatc ccaacgaccg aaaacctgta ttttcagggc
     5641 gccatgggat ccatgcagat cttcgtgaag actctgactg gtaagaccat caccctcgag
     5701 gtggagccca gtgacaccat cgagaatgtc aaggcaaaga tccaagataa ggaaggcatt
     5761 cctcctgatc agcagaggtt gatctttgcc ggaaaacagc tggaagatgg tcgtaccctg
     5821 tctgactaca acatccagaa agagtccacc ttgcacctgg tactccgtct cagaggtggg
     5881 tgagaattct aaggaaatcc attatgtctt cctccaagcg tattgccaag gaattaagtg
     5941 atttagggag agatcctcct gcttcatgtt cagcaggacc tgtaggggat gacctgtatc
     6001 attggcaagc ctctattatg ggtccttcag actcacccta cgctggtggc gttttctttt
     6061 tgtctattca ctttccaact gattatccat tcaagccacc gaaggtaaac tttacgacca
     6121 aaatttatca tccgaatatt aattcgagtg gtaatatctg ccttgatatt ttaaaggacc
     6181 agtggtcacc ggcgctaacc ctttcaaaag ttttgttgtc tatttgctct cttttaacag
     6241 atgctaatcc cgacgatcct ttggtccctg aaattgctca aatctacaag acagataagg
     6301 ctaagtatga agccaccgct aaggagtgga ctaaaaaata tgctgtttga ggcgcgccac
     6361 aattccgaca ggaaacagct atgcttcccc ggaagcggga gatcgtcgcc ggcgaggtcg
     6421 aggacttgca gaagaagacc cgcgccgggg agggggaggt cactagggag gaaggagacg
     6481 cagccatggc agggaggggg aacgagatcg acgaggacct gcacagccgc cagctcgccg
     6541 tctatgggcg cgagaccatg aagcgcctct tcggctccaa cgtcctcgtc tctggactcc
     6601 agggtctcgg tgctgagatc gcaaagaacc ttgtccttgc gggtgtcaag tctgtaacct
     6661 tgcatgatga tggtaacgtg gagctgtggg acttatcaag caacttcttc ctctcggaga
     6721 atgatgttgg gcaaaaccgt gctcaagctt gtgtacagaa gctacaagag ctgaacaatg
     6781 ctgtcctcgt ctctgcctta accggtgatt tgaccaagga gcacctttct aaattccagg
     6841 ccgttgtctt cactgatatc agcttagata aagcaattga atttgatgat tactgccaca
     6901 gccaacagcc acccattgcg ttcatcaagt ctgaagttcg tggtcttttt ggcagtgttt
     6961 tttgtgactt tggtcctgaa tttactgttt tggatgtgga tggagaggaa ccacatacag
     7021 gaattgttgc atcaatcagc aatgacaatc cagcacttgt atcttgtgtg gacgatgagc
     7081 gtctggagtt ccaggatggt gatctagttg ttttctctga agtccatgga atgactgagc
     7141 tgaatgatgg aaaaccaaga aaagttaaga atgcaaggcc ttactctttc ttcttggaag
     7201 aagacacttc ctcatttggc gcatatgtta gaggtggtat tgtcacacag gtcaagccac
     7261 cgaaggttat taaattcaaa cccttgaaag aggccatgtc agagccggga gaatttctca
     7321 tgagtgattt ctccaaattt gagcggccac ctcttctgca tttggcattc caagctttgg
     7381 ataagtttag gactgagttg agcagattcc ctgttgctgg gtccactgat gatgtgcaaa
     7441 gggtgataga gtatgctatt agcattaatg atactctggg agataggaaa cttgaagaaa
     7501 ttgacaaaaa gctcctgcat cattttgcca gtggttccag ggctgttctg aatcctatgg
     7561 ctgcgatgtt tggtggtatt gtgggtcagg aggtagtgaa agcttgctca gggaaatttc
     7621 atccacttta tcagttcttc tattttgatt ctgtcgagtc tctccctgtt gaccccttgg
     7681 agcctggtga tttgaagccg aagaacagta gatatgatgc tcaaatcagc gtatttggat
     7741 ctaagcttca aaacaaactg gaggaagcaa aaatctttat ggtcggttct ggggcacttg
     7801 gatgtgagtt cttgaagaac ctggcactaa tgggtatttc ttgcagccag aatggaaatc
     7861 tgactctgac agatgatgat gtcattgaaa agagtaatct gagccgccaa tttcttttcc
     7921 gtgactggaa cattgggcaa cctaagtcca cagttgctgc tacagctgct atggtaatta
     7981 atcctaaact tcatgtcgag gcccttcaga acagagcaag tcctgagact gaaaatgtgt
     8041 ttaatgatgc cttctgggag aaccttgatg ctgttgtcaa tgcccttgac aatgttactg
     8101 caagaatgta catagactcc agatgtgtat atttccagaa gccacttttg gaatctggga
     8161 ctctgggtgc taaatgcaat acacagatgg tcatccctca cctaacagag aactatgggg
     8221 cttcaaggga tccacctgaa aagcaggcac ctatgtgcac tgtacattca tttcctcata
     8281 acattgatca ttgcctaacc tgggctaggt ctgagtttga gggtttactt gagaagactc
     8341 ccactgaagt aaatgctttc ctgtcaaatc ctactacata cataagtgca gcaagaactg
     8401 ctggtgatgc acaggctaga gatcagcttg aacgtgttat tgagtgtctt gacagagaca
     8461 agtgcgagac gttccaggat tctattacct gggcccggct taagtttgag gattacttct
     8521 ccaaccgtgt gaagcagctg acgttcactt tcccagaaga ctcgatgacc agctctggtg
     8581 ctcctttctg gtctgctcca aagcggttcc cacgacctgt ggagttctcg tccagtgatc
     8641 agagtcagct tagctttatt ttggctgctg caatattgag ggcagagact tttggaatac
     8701 ccatacctga gtgggccaaa accccaaaca agctggctgc tgaagctgtc gacaaggtga
     8761 ttgtcccgga ttttcaacca aagcaggggg ttaagatagt tacagatgag aaggccacta
     8821 gtctgtcctc tgcatctgtt gacgacgctg ctgtcattga agagcttatt gccaagttag
     8881 aagaagtttc caaaacactg ccatcagggt tccacatgaa cccaatacag tttgagaagg
     8941 atgatgacac aaacttccat atggatgtga tagctggttt tgccaacatg agggcaagga
     9001 actacagcat tcctgaagtg gacaagctca aggccaagtt tatagccggc aggatcatcc
     9061 cagctatcgc cacctccacc gcgatggcca ctggcctcgt ctgccttgag ctttacaaag
     9121 cccttgctgg tggacacaag gttgaagact accgcaacac gtttgcaaac ctcgctatcc
     9181 ctctcttctc cattgctgaa ccagttccac ccaagaccat caagcaccag gagttgtcgt
     9241 ggacggtctg ggaccggtgg actgtgacgg gcaacatcac gctgagggag ctcctggagt
     9301 ggctcaagga gaagggcctg aacgcttaca gcatatcctg tggcacctcg ctgctgtaca
     9361 actccatgtt ccccaggcac aaggaacggc ttgacaggaa ggtagttgat gttgccaggg
     9421 aggtggccaa gatggaggtg ccctcttacc ggcgtcatct cgacgtcgtg gtggcgtgtg
     9481 aggatgacga cgacaatgat gtcgacatcc cacttgtgtc ggtctacttc cgctgattcg
     9541 aatctagagc ctgcagtctc gagcaccacc accaccacca ctgagatccg gctgctaaca
     9601 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
     9661 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
//
