LOCUS       Exported                7972 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pDM#834
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7972)
  AUTHORS   Meissner B, Rogalski T, Viveiros R, Warner A, Plastino L, Lorch A, 
            Granger L, Segalat L, Moerman DG
  TITLE     Determining the Sub-Cellular Localization of Proteins within 
            Caenorhabditis elegans Body Wall Muscle.
  JOURNAL   PLoS One. 2011  . 6(5):e19937.
  PUBMED    21611156
REFERENCE   2  (bases 1 to 7972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7972
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(79..95)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(79..95)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(92..114)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    103..119
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(127..157)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    172..193
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(310..327)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(481..1069)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(561..580)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1240..2100)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1863..1882
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2101..2205)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2273..2291
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2369..2388)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2488..2510
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3477..3497)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     4245..4273
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    4326..4350
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6004..6028)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttttaagct ctgaaaattt aaatgagaat agttagttag taagatcatg tttaaactta
       61 cagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
      121 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
      181 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
      241 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
      301 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
      361 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
      421 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      481 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      541 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      601 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      661 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      721 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      781 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      841 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      901 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      961 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1021 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1081 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1141 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1201 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     1261 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     1321 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     1381 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     1441 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1501 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1561 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1621 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1681 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1741 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1801 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1861 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1921 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1981 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     2041 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     2101 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     2161 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     2221 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     2281 tatcacgagg cccttaaggc cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     2341 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     2401 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     2461 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     2521 catcaccgaa acgcgcgaga cgaaagggcc cgtacggccg actagtagga aacagttatg
     2581 tttggtatat tgggaatgta ttctgtcatt taaggcaaaa accccataga cactactcca
     2641 ctttcaaatt atcaacttct actcaccttc cactgagcct caaacccaaa ccttcttccg
     2701 atctttcttg catcgtgctc atcaatactt gtgattttag caagaaaaac gccgtcctcg
     2761 aaatcttgac aaacaatagg ggaaacccca aaaagcaaaa gcaggaaaat taatttcata
     2821 aactttcact tgattgaaaa attccaaaaa tactccaaaa ctttttcacg atgagcatga
     2881 tttgacgtca tgagaggccc agacgtgcga agaaataaaa attgcggtca taaactgaaa
     2941 cgtaacatat gataaggtat tttgtgtgcg gttttttcta tgatgtttca aaaaaattta
     3001 tcagaagtaa aaaacataag aagtgtacac agaacattgt gtagattttg ggatagaatt
     3061 attattaatg gggggaggga gcacaatttt tttcgacttt ttattacgaa ttaattctat
     3121 ttttgaatct acacaatttc attgttagag gtgacttaaa agaagctaaa aaacaaagaa
     3181 attaagagaa gttttttgat aataacaaaa atagggggtg ggagcacagg gagaaagagc
     3241 atgtagggat gttgaagagt aattggactt agaagtcaga ggcacgggcg cgagatgctg
     3301 aaaatttaaa tattactcgt tagttagtag aactcagttt aaacttactc tgatgacagc
     3361 ggcccctatt atttttgaca ccagacaagt tggtaatggt agcgaccggc gctcagttgg
     3421 aattctacga atgctatttg tatagttcat ccatgccatg tgtaatccca gcagctgtta
     3481 caaactcaag aaggaccatg tggtctctct tttcgttggg atctttcgaa agggcagatt
     3541 gtgtggacag gtaatggttg tctggtaaaa ggacagggcc atcgccaatt ggagtatttt
     3601 gttgataatg gtctgctagt tgaacgcttc catcttcaat gttgtgtcta attttgaagt
     3661 tctgaaaatt taaatcagat tagttagtta gtaaaatcat gtttaaactt acaactttga
     3721 ttccattctt ttgtttgtct gccatgatgt atacattgtg tgagttatag ttgtattcca
     3781 atttgtgtcc aagaatgttt ccatcttctt taaaatcaat accttttaac tcgattctat
     3841 taacaagggt atcaccttca aacttgactt cagcacctga aaatttaaat atgtatggtt
     3901 agttagtacc gaactgttta aacttacgtg tcttgtagtt cccgtcatct ttgaaaaata
     3961 tagttctttc ctgtacataa ccttcgggca tggcactctt gaaaaagtca tgccgtttca
     4021 tatgatctgg gtatctcgag aagcattgaa caccataaca gaaagtagtg acaagtgttg
     4081 gctgaaaatt taaataatca gggttagtta gtatatatat gtttaaactt acccatggaa
     4141 caggtagttt tccagtagtg caaataaatt taagggtaag ttttccgtat gttgcatcac
     4201 cttcaccctc tccactgaca gaaaatttgt gcccattaac atcaccatct aattcaacaa
     4261 gaattgggac aactccagtg aaaagttctt ctcctttact cattttttct accggtaccc
     4321 ccctcaccac tttgtacaag aaagctgggt agataatcac gtatttcggc aagtttggat
     4381 ttatcttgct cttcgcatcc aaatattttt tgaacttcgc caagaatagt ggcaaatcaa
     4441 atgagaagtc agtgttgagg agtacagcat gatcaacgaa gtccaattcg gtatgctcta
     4501 gttcgactcc gcagaatatt ggcagtttga ctccacgacg gttcatcgca aagcgcagac
     4561 gctttttgac ttgaggccag agtacacgtg ggaccacaaa tgagaagagc ttttcgattg
     4621 gagcagcgag tggtccgcca agtccaagat caaaaacgtt gacatccaca ttagtcagag
     4681 aaacatctcc aaaaatacgc gaatgccgga cagttggctt cagcgtgacg tgcaactttg
     4741 tgttagcatg aagaacatca tggagttgtt ctcgtccatg gaacatgatt gagacatttc
     4801 cagacaattg aactcgtgct ccaagatgag catcgatttg gagaatcggt tgttcagtaa
     4861 gattagcgga aacttccacg tagcattttg agcacaccgc ctttgaaagt ttttgcagta
     4921 tttttggaag tgaacttggg gtgtatctat gtttgaaatt tcccaagtca tacatgtaaa
     4981 tatgatgaaa cagtgtattg gggatgtact cggagagaag aattccaacc attttcttat
     5041 caagccgggt cttgttcaaa atttgaagct gctgagctga gtcggccttc cagccgttga
     5101 agagaattcc tccatcaagg ccaataatca tatcctcatc tgtaaatgtt ggtggatgat
     5161 taacgctcaa atcaactccc caataagatt tttgagtttc ctcggttgtc aacgttgagt
     5221 tatcatcatc cggtgttgga gcaaccgttg tctccacatt tccctcttct tgctccgaat
     5281 cttcaaatcc attaactgtg tcaacttggc acaatgatgc tcctaactcg gcgacactga
     5341 aaccatttgg atctcctgct tctggcttct gatagtttgg gctctcaggt ggaattggtt
     5401 tctgtgccat cacgaatgtg agagcttcaa cagcggttgt gcagacagca tcacggagag
     5461 cagagacaat ctgtccttta atttccgatt gaagtgctga caaacttgat ccaggtggaa
     5521 acaccagtga aagttcggaa tgagttacgt tgcaaacgga agcattaaca gtagtagttc
     5581 cattataatc aaaatgaagt tccgattcga tagacagttc gcggagctct gcagtgaaat
     5641 ctccttcata tgaccacaca aatacactga tctcagcata agcacttgca cggaaggaag
     5701 ccaatgatat attgtaggca acaccttttc catcaatgaa ttttgcgctg aggttcggct
     5761 ttgcaacgtt tgatatatga agctttgtga ttttagccat atcagtcaca atatcgggag
     5821 ggctcaagtt attgagaatt gctgggagag catcggatgc caaatctgcg acataatcca
     5881 ctgctttttg atttaatcga acgtagattc ctgctggttt atgtgtaatt tccgaactgt
     5941 cagaagaatt tcctacttga attattgaaa caagcaggaa aaatatgtga aaattccgcg
     6001 acaagcctgc ttttttgtac aaacttgtgg cccaatcccg gggcattttc tgaattaata
     6061 ttgaatcgtt tattatattt ttatcacatt ttaaggcata gaagtgtttt ggaaacaagg
     6121 aaatcaactt ttttaaattt aaaacttata ttgaagtgca aaccattggg gtacggaaaa
     6181 tacagagaag aagatgaatt gtcaaatatc atagcaatac ttttttattt aaaaaaattg
     6241 aaattggttc catctatatc caaaaattgc aagttctcaa caattttgga agtcaaccaa
     6301 ataattaaag atttttttaa attgtttttg tttctaatta tgtttaaagc atttgtactc
     6361 atacaaacat aggtctaaca ctttttcaaa aaaaaaaanc caacaattcc ccaacaaaaa
     6421 attaaagtct aacaattttg gacattcctg caggttcgaa gttctatgat tcttgaattt
     6481 aaatacaaaa ttcgtaggac ttttttggtc aaaattacaa attccaaatt atgataggtg
     6541 aaaactattc caattttttg acatgaaatt ctaaaaaatt ctaataaact accatggctt
     6601 ttgtaaattt cggcatcatt gaaagaatat gaagttttat tttttagatt atttgaaata
     6661 agaaaaaatt attttttcaa aattactgta tcattcgaga aattgaaact caccaaaaag
     6721 gtgaaaattt aacttgaata ttgatttttg attaaattca gtttaaagaa tttttttaaa
     6781 aaataggaat tcgaaattta cggtactatt acggtacttg gtctcgaagc gaaacgaata
     6841 atttgaagtg gaaaaaaaat tcaaggaaac tttcaaaaaa aactactgta cttcttaaag
     6901 gcgcacactc gttttcattt caatttattt atattttctt cggtcgtcgc gagaccggag
     6961 accctaattt tgcaatgaaa atcgtgcaac ttcgcacata ggatacaagt actatttcgt
     7021 atttttgatt tttcaggtac tctaactaat tctagatgct gtttataaaa gtatatccaa
     7081 aaaaaagttc atagcaaaaa tgtttttttt ttcaggattt ctagctacaa aaccaaattt
     7141 ttaaaagtag tagaacaaat cttaagaagt tatcaagtag tacaaactaa ttagaaaaac
     7201 attgctcttg gagcaagaag gcactagtga tgaatcagtt gcgacatgtt gtgaatcttc
     7261 gacttcttcc tgtgtcccgc cactaaaaca cgcaaaatga gccatcgacg ggaccctgca
     7321 tctccgtgat tcattgaatc tttcatcatt tggaactcaa aatgacgacg ggacgacaag
     7381 tggctcctgg acgggaagaa acgaaaagaa ataagaagat gagacgtatg tgtgtgtgtg
     7441 tccactactc tttttttctc ggcatcccct cgttttatac ccaggttttc gtaatactca
     7501 actcggaatt tgaaactgag acgttggcac ggctcaccta caacctttgt tgagctcata
     7561 aaacctgcta cgaacaggtg tcgacctaga gctactagat gacccgtgta gagggatggc
     7621 tcttatttat atgaaacaag aactttctga attcaaaaat tattttaatc ttttttcgag
     7681 gctgtactgt caatggtttt tttataaaaa ctatttagcg gacccaaaat tggtctgcta
     7741 accagtttga gaattttaaa gtaaaaacgc tattaacagt actccatcat aaaaacttat
     7801 tggaatcgta aaatgaacag ttatacaaca tgctggttga tgcaacaaat ggaacaaata
     7861 ctcatcaaat tctcaggctt tgtttgcgaa tgtgaaggca agccggagat aggaaaagct
     7921 tatttcattt ccaagttgtt agcgtatcca tcgttgtgag tgatttcagc ca
//
