LOCUS Exported 6668 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS HA-TAZ SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6668) AUTHORS Lei QY, Zhang H, Zhao B, Zha ZY, Bai F, Pei XH, Zhao S, Xiong Y, Guan KL TITLE TAZ promotes cell proliferation and epithelial-mesenchymal transition and is inhibited by the hippo pathway. JOURNAL Mol Cell Biol. 2008 Apr;28(7):2426-36. Epub 2008 Jan 28. PUBMED 18227151 REFERENCE 2 (bases 1 to 6668) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6668 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(40..60) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 45..374 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 225..360 /label=SV40 ori /note="SV40 origin of replication" primer_bind 287..306 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 441..1235 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(495..514) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 1105..1124 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 1409..1530 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1446..1465) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1500..1519 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(1579..1595) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1579..1595) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1592..1614) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 1603..1619 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1627..1657) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 1672..1693 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(1810..1827) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1981..2569) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2061..2080) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2740..3600) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3363..3382 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3601..3705) /gene="bla" /label=AmpR promoter primer_bind complement(3780..3799) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 3971..4350 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 4351..4554 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 4505..4525 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 4599..4618 /label=T7 /note="T7 promoter, forward primer" promoter 4599..4617 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 4640..4666 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 4670..4696 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" primer_bind complement(5926..5942) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5927..5943) /label=pBluescriptKS /note="For pBluescript vector" promoter complement(5957..5975) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(5958..5975) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(5995..6012) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6001..6225 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 6271..31 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6358..6377) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6568..6589 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt 61 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 121 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 181 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 241 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 301 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 361 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga 421 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg 481 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg 541 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt 601 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg 661 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat 721 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat 781 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg 841 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg 901 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc 961 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc 1021 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg 1081 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg 1141 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca 1201 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac 1261 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga 1321 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga 1381 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa 1441 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 1501 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta 1561 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 1621 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 1681 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 1741 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 1801 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 1861 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 1921 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 1981 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 2041 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 2101 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 2161 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 2221 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 2281 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 2341 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 2401 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 2461 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 2521 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 2581 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 2641 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 2701 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 2761 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 2821 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 2881 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 2941 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 3001 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 3061 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 3121 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 3181 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 3241 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 3301 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 3361 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 3421 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 3481 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 3541 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 3601 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 3661 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 3721 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt 3781 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag 3841 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat 3901 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga 3961 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta 4021 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc 4081 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg 4141 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg 4201 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa 4261 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac 4321 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 4381 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 4441 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 4501 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg 4561 ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag 4621 acccaagctt gccatggcct acccatacga tgttcctgac tatgcgggct atccctatga 4681 cgtcccggac tatgcaggat ccatgaatcc ggcctcggcg ccccctccgc tcccgccgcc 4741 tgggcagcaa gtgatccacg tcacgcagga cctagacaca gacctcgaag ccctcttcaa 4801 ctctgtcatg aatccgaagc ctagctcgtg gcggaagaag atcctgccgg agtctttctt 4861 taaggagcct gattcgggct cgcactcgcg ccagtccagc accgactcgt cgggcggcca 4921 cccggggcct cgactggctg ggggtgccca acatgtccgc tcgcactcgt cgcccgcgtc 4981 cctgcagctg ggcaccggcg cgggtgctgc gggtagcccc gcgcagcagc acgcgcacct 5041 ccgccagcag tcctacgacg tgaccgacga gctgccactg cccccgggct gggagatgac 5101 cttcacggcc actggccaga ggtacttcct caatcacata gaaaaaatca ccacatggca 5161 agaccctagg aaggcgatga atcagcctct gaatcatatg aacctccacc ctgccgtcag 5221 ttccacacca gtgcctcaga ggtccatggc agtatcccag ccaaatctcg tgatgaatca 5281 ccaacaccag cagcagatgg cccccagtac cctgagccag cagaaccacc ccactcagaa 5341 cccacccgca gggctcatga gtatgcccaa tgcgctgacc actcagcagc agcagcagca 5401 gaaactgcgg cttcagagaa tccagatgga gagagaaagg attcgaatgc gccaagagga 5461 gctcatgagg caggaagctg ccctctgtcg acagctcccc atggaagctg agactcttgc 5521 cccagttcag gctgctgtca acccacccac gatgacccca gacatgagat ccatcactaa 5581 taatagctca gatcctttcc tcaatggagg gccatatcat tcgagggagc agagcactga 5641 cagtggcctg gggttagggt gctacagtgt ccccacaact ccggaggact tcctcagcaa 5701 tgtggatgag atggatacag gagaaaacgc aggacaaaca cccatgaaca tcaatcccca 5761 acagacccgt ttccctgatt tccttgactg tcttccagga acaaacgttg acttaggaac 5821 tttggaatct gaagacctga tccccctctt caatgatgta gagtctgctc tgaacaaaag 5881 tgagcccttt ctaacctggc tgtaagaatt cgatatcaag cttatcgata ccgtcgacct 5941 cgaggggggg ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga 6001 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc 6061 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc 6121 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt 6181 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa 6241 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg 6301 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 6361 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 6421 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 6481 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 6541 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 6601 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt 6661 taaaaaat //