LOCUS Exported 5352 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: Nucleus/Histones, Excitation: 684, Emission: 708. ACCESSION . VERSION . KEYWORDS IFP2.0-H2B-6 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5352) TITLE Davidson Red fluorescent proteins REFERENCE 2 (bases 1 to 5352) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5352 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1..378 /codon_start=1 /gene="HIST1H2BJ" /product="human histone H2B" /label=H2B /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP GELAKHAVSEGTKAITKYTSAK" regulatory 391..400 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 397..1362 /codon_start=1 /product="bacteriophytochrome-based monomeric infrared fluorescent protein (Yu et al., 2014)" /label=IFP2.0 /translation="MARDPQPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTAD GHSGEVLQVSLNAATFLGQEPTVLRGQTLAALLPDQWPALQTALPPGCQDALQYRATLD WPAAGHLSLTVHRVAELLILEFEPTEAWDSIGPHALRNAMFALESAPNLRALAEVATQT VRELSGFDRVMLYKFAPDATGEVIAEARREGMQAYLGHRFPASTTPAQARALYTRHLLR LTADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGG QLWGLIVCHHQTPYVLPPDLRTTLEYLGRLLSLQVQRKEA" polyA_signal 1484..1605 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1521..1540) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1575..1594 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1612..2067) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1749..1770) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 1961..1980 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2094..2198 /gene="bla" /label=AmpR promoter promoter 2200..2557 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2223..2243) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2408..2543 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2470..2489 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2592..3386 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2646..2665) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3256..3275 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3574..3593) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3618..3665 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 3994..4582 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4483..4502 /label=pBR322ori-F /note="pBR322 origin, forward primer" enhancer 4757..5060 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 5061..5264 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 5215..5235 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" ORIGIN 1 atgccagagc cagcgaagtc tgctcccgcc ccgaaaaagg gctccaagaa ggcggtgact 61 aaggcgcaga agaaaggcgg caagaagcgc aagcgcagcc gcaaggagag ctattccatc 121 tatgtgtaca aggttctgaa gcaggtccac cctgacaccg gcatttcgtc caaggccatg 181 ggcatcatga attcgtttgt gaacgacatt ttcgagcgca tcgcaggtga ggcttcccgc 241 ctggcgcatt acaacaagcg ctcgaccatc acctccaggg agatccagac ggccgtgcgc 301 ctgctgctgc ctggggagtt ggccaagcac gccgtgtccg agggtactaa ggccatcacc 361 aagtacacca gcgctaagga tccaccggtc gccaccatgg ctcgggaccc tcaacctttc 421 ttccctcctc tgtatctcgg aggaccagag atcactactg agaactgcga gcgggaacct 481 atacatatac caggttctat tcaacctcat ggagcactgc tcacagccga tggacattct 541 ggcgaggtgc tgcaagtttc actcaacgca gccactttcc tgggccagga accaactgtt 601 ctgcggggac agaccctggc agccctgctg ccagaccagt ggcctgctct gcagactgcc 661 ctgcctccag gctgccagga cgccctgcag tatcgcgcca ccctggattg gcccgccgcc 721 ggacacctga gcctgaccgt gcatagggtg gccgagctgc tgattctgga attcgagcct 781 actgaggcat gggactctat tggaccccac gctctgagaa atgcaatgtt cgctctggag 841 agtgctccta atctccgggc actggctgaa gtcgcaaccc aaacagtccg ggaactgtca 901 ggtttcgacc gggtgatgct gtacaagttt gcaccagacg caacaggaga ggttattgcc 961 gaagcaaggc gcgagggcat gcaggcttac ctcgggcata ggtttcccgc atccaccacc 1021 cctgcacaag ctagggccct ctacacaaga cacctgctcc ggctgaccgc agacaccagg 1081 gctgcagcag tgcccctcga ccccgtgctg aatccccaga caaatgctcc tacacctctg 1141 ggcggagctg tcctcagagc tacatcccca atgcacatgc agtacctgag gaatatggga 1201 gtgggctcct ccctgagcgt cagcgtcgtg gtcggcggcc agctgtgggg actgattgtc 1261 tgccaccatc agacacccta cgtgctgcca cccgatctgc ggaccaccct ggagtatctg 1321 gggaggctcc tgtccctgca ggtgcagagg aaagaagcct gagcggccgc gactctagat 1381 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct 1441 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc 1501 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 1561 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg taaattgtaa 1621 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc 1681 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga 1741 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag 1801 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt 1861 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta 1921 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag 1981 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg 2041 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa 2101 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 2161 cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct 2221 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 2281 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc 2341 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac 2401 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 2461 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta 2521 gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag 2581 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg 2641 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt 2701 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc 2761 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt 2821 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag 2881 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg 2941 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag 3001 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg 3061 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga 3121 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca 3181 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc 3241 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg 3301 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct 3361 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc 3421 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg 3481 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct 3541 ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga 3601 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt 3661 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc 3721 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg 3781 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta 3841 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 3901 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 3961 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 4021 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 4081 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 4141 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 4201 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 4261 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 4321 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 4381 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 4441 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 4501 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 4561 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 4621 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 4681 tattaccgcc atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc 4741 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 4801 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 4861 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 4921 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 4981 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 5041 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 5101 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 5161 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 5221 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc 5281 agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac 5341 ggtaccgcca cc //