LOCUS Exported 5385 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses p12 and p9-mRFP1. ACCESSION . VERSION . KEYWORDS pCAM10 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5385) AUTHORS Myhrvold C, Kotula JW, Hicks WM, Conway NJ, Silver PA TITLE A distributed cell division counter reveals growth dynamics in the gut microbiota. JOURNAL Nat Commun. 2015 Nov 30;6:10039. doi: 10.1038/ncomms10039. PUBMED 26615910 REFERENCE 2 (bases 1 to 5385) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5385 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 23..307 /gene="araBAD" /label=araBAD promoter /note="promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)" primer_bind 230..249 /label=pBAD Forward /note="For vectors with E. coli araBAD promoter, forward primer" RBS 318..329 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" CDS 1190..1867 /codon_start=1 /product="monomeric derivative of DsRed (Campbell et al., 2002)" /label=mRFP1 /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS TGA" terminator 1885..1956 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 1972..1999 /label=T7Te terminator /note="phage T7 early transcription terminator" CDS 2040..2069 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 2085..2102 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(2158..2175) /label=pBAD Reverse /note="For vectors with E. coli araBAD promoter, reverse primer" terminator 2328..2374 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind complement(2416..2433) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2587..3175) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2667..2686) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3346..4206) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3969..3988 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(4207..4311) /gene="bla" /label=AmpR promoter primer_bind 4379..4397 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 4401..4444 /label=bacterial terminator /note="putative bacterial transcription terminator" CDS complement(4503..5381) /codon_start=1 /gene="araC" /product="L-arabinose regulatory protein" /label=araC /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSRLFRQQLGIS VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE EKVNDVAVKLS" ORIGIN 1 ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 61 cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 121 attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 181 cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 241 tttttatcca taagattagc ggatcttacc tgacgctttt tatcgcaact ctctactgtt 301 tctccatacc cgctagcaaa gaggagaaaa gaatggttat cggtctcctg aagtatctca 361 cgcctgccgt taaggtgcag atggctgctc gcgcgttggg cctgtccccc gccgaagtcg 421 ctgcaattga cggcacgttg ggtcgtgtct ctgcgatgcc agcggtcgcg gtcgtgctgg 481 gagggaaacc tctctctctg gccacgatcg cgtcagttgt gtctgatgca aaccccagtg 541 ccactgttgg cgcgcttatg cctgctgtac agggcatggt gagttccgac gaaggcgcga 601 gtgcgttggc taagaccgtg gtaggcttca tggagtccga ccccaacagc gatgtcctgg 661 ttcaactgct ccacaaggtg tcaaacttgc cgattgtcgg ctttggtgac acgcagtatg 721 cagacccagc tgacttcttg gccaagggag ttttccctct gatcaggaag ccagaagtag 781 aggttcaagc tgcgcctttc acctgtcgtc agtgtgatca tgttgatcac atcactgatg 841 tacctcaaac ttcgaccttt gttcacaaat gcacttcgtg cggctttgtg cagatggtcc 901 accgtaagga tgttccgtaa tgccatttcc tctggtaaag caagacccaa cctcgaaggc 961 tttcactgaa gccagtgaac gctccaccgg cacccagatc ctggacgtcg tcaaggcccc 1021 tatcggcctg ttcggcgacg atgccaaaca cgagttcgtg acccgtcagg aacaagccgt 1081 ctccgtcgtc agctgggcag ttgctgccgg tctgatcggc gagctgatcg gctaccgtgg 1141 tgcgcgttcg ggtcgcaaag cgatcctggc caacatccct ttcctggcca tggcgagtag 1201 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg 1261 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc 1321 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca 1381 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa 1441 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt 1501 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact 1561 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga 1621 agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg 1681 tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa 1741 aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca 1801 caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg 1861 tgcttaataa ggatctccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 1921 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 1981 tcgggtgggc ctttctgcgt ttataggatc ctaactcgag taatgagaag cttgggcccg 2041 aacaaaaact catctcagaa gaggatctga atagcgccgt cgaccatcat catcatcatc 2101 attgagttta aacggtctcc agcttggctg ttttggcgga tgagagaaga ttttcagcct 2161 gatacagatt aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggcag 2221 tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga 2281 tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa 2341 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aactaatttc 2401 tagactgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 2461 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 2521 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 2581 ggcgtttttc cacaggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 2641 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 2701 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 2761 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 2821 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 2881 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 2941 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 3001 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 3061 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 3121 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 3181 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 3241 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 3301 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 3361 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 3421 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 3481 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 3541 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 3601 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 3661 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 3721 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 3781 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 3841 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 3901 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 3961 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 4021 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 4081 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 4141 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 4201 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 4261 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 4321 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 4381 taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga 4441 tttctggaat tcactagtct atgctactcc atcgagccgt caattgtctg attcgttacc 4501 aattatgaca acttgacggc tacatcattc actttttctt cacaaccggc acggaactcg 4561 ctcgggctgg ccccggtgca ttttttaaat acccgcgaga aatagagttg atcgtcaaaa 4621 ccaacattgc gaccgacggt ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc 4681 tggctgatac gttggtcctc gcgccagctt aagacgctaa tccctaactg ctggcggaaa 4741 agacgtgaca gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa 4801 ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg attatccatc 4861 ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca gcaacaattg ctcaagcaga 4921 tttatcgcca gcagctccga atagcgccct tccccttgcc cggcgttaat gatttgccca 4981 aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaccc cgtattggca 5041 aatattgacg gccagttaag ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac 5101 tggtgatacc attcgcgagc ctccggatga cgaccgtagt gatgaatctc tcctggcggg 5161 aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc cctgattttt caccaccccc 5221 tgaccgcgaa tggtgagatt gagaatataa cctttcattc ccagcggtcg gtcgataaaa 5281 aaatcgagat aaccgttggc ctcaatcggc gttaaacccg ccaccagatg ggcattaaac 5341 gagtatcccg gcagcagggg atcattttgc gcttcagcca tactt //