LOCUS       Exported                4810 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Expresses pduA-mRFP1.
ACCESSION   .
VERSION     .
KEYWORDS    pCAM69
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Myhrvold C, Kotula JW, Hicks WM, Conway NJ, Silver PA
  TITLE     A distributed cell division counter reveals growth dynamics in the 
            gut microbiota.
  JOURNAL   Nat Commun. 2015 Nov 30;6:10039. doi: 10.1038/ncomms10039.
  PUBMED    26615910
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        22..306
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     229..248
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             317..328
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             614..1291
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      1309..1380
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1396..1423
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             1464..1493
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1509..1526
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1582..1599)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1752..1798
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1840..1857)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2011..2599)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2091..2110)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2770..3630)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3393..3412
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3631..3735)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3803..3821
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      3825..3868
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             complement(3927..4805)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSRLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 tcatactccc gccattcaga gaagaaacca attgtccata ttgcatcaga cattgccgtc
       61 actgcgtctt ttactggctc ttctcgctaa ccaaaccggt aaccccgctt attaaaagca
      121 ttctgtaaca aagcgggacc aaagccatga caaaaacgcg taacaaaagt gtctataatc
      181 acggcagaaa agtccacatt gattatttgc acggcgtcac actttgctat gccatagcat
      241 ttttatccat aagattagcg gatcttacct gacgcttttt atcgcaactc tctactgttt
      301 ctccataccc gctagcaaag aggagaaaag aatgcaacaa gaagcactag gaatggtaga
      361 aaccaaaggc ttaaccgcag ccatagaggc cgctgatgca atggttaagt cagccaatgt
      421 gatgttagtg ggctatgaaa agattggctc cgggctggta accgtcatcg tgcgcggcga
      481 tgttggcgcg gtcaaagcgg ccaccgatgc aggtgccgca gccgcacgca acgtgggtga
      541 agtgaaagcc gtacacgtca tcccacgccc tcacaccgat gtagaaaaaa tcttaccgaa
      601 gggaattagc caaatggcga gtagcgaaga cgttatcaaa gagttcatgc gtttcaaagt
      661 tcgtatggaa ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg
      721 tccgtacgaa ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt
      781 cgcttgggac atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc
      841 ggctgacatc ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt
      901 tatgaacttc gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg
      961 tgagttcatc tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat
     1021 gcagaaaaaa accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc
     1081 tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga
     1141 agttaaaacc acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga
     1201 catcaaactg gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg
     1261 tgctgaaggt cgtcactcca ccggtgctta ataaggatct ccaggcatca aataaaacga
     1321 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1381 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatag gatcctaact
     1441 cgagtaatga gaagcttggg cccgaacaaa aactcatctc agaagaggat ctgaatagcg
     1501 ccgtcgacca tcatcatcat catcattgag tttaaacggt ctccagcttg gctgttttgg
     1561 cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat
     1621 aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc
     1681 agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa
     1741 ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1801 gttgtttgtc ggtgaactaa tttctagact gcaggcttcc tcgctcactg actcgctgcg
     1861 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     1921 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     1981 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
     2041 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2101 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2161 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2221 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2281 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2341 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2401 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     2461 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2521 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2581 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2641 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     2701 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     2761 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     2821 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     2881 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     2941 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     3001 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     3061 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     3121 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     3181 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     3241 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     3301 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     3361 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     3421 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     3481 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     3541 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     3601 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     3661 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     3721 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     3781 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
     3841 atccttagct ttcgctaagg atgatttctg gaattcacta gtctatgcta ctccatcgag
     3901 ccgtcaattg tctgattcgt taccaattat gacaacttga cggctacatc attcactttt
     3961 tcttcacaac cggcacggaa ctcgctcggg ctggccccgg tgcatttttt aaatacccgc
     4021 gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg
     4081 gtggtgctca aaagcagctt cgcctggctg atacgttggt cctcgcgcca gcttaagacg
     4141 ctaatcccta actgctggcg gaaaagacgt gacagacgcg acggcgacaa gcaaacatgc
     4201 tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat gtactgacaa
     4261 gcctcgcgta cccgattatc catcggtgga tggagcgact cgttaatcgc ttccatgcgc
     4321 cgcagcaaca attgctcaag cagatttatc gccagcagct ccgaatagcg cccttcccct
     4381 tgcccggcgt taatgatttg cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc
     4441 gggcgaaaga accccgtatt ggcaaatatt gacggccagt taagccattc atgccagtag
     4501 gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg atgacgaccg
     4561 tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca aacaaattct
     4621 cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat ataacctttc
     4681 attcccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa
     4741 cccgccacca gatgggcatt aaacgagtat cccggcagca ggggatcatt ttgcgcttca
     4801 gccatacttt
//
