LOCUS       Exported                4373 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Expresses eutM-mRFP1.
ACCESSION   .
VERSION     .
KEYWORDS    pCAM70
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Myhrvold C, Kotula JW, Hicks WM, Conway NJ, Silver PA
  TITLE     A distributed cell division counter reveals growth dynamics in the 
            gut microbiota.
  JOURNAL   Nat Commun. 2015 Nov 30;6:10039. doi: 10.1038/ncomms10039.
  PUBMED    26615910
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        7..291
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     214..233
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             302..313
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             605..1282
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGTVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSNNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      1300..1346
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1388..1405)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1559..2147)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1639..1658)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2318..3178)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSKATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2941..2960
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3179..3283)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      3373..3416
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             complement(3475..4353)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFQPGEIHHYGRHPEAREWYHKWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSRLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc gtcttttact
       61 ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa aagcattctg taacaaagcg
      121 ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc
      181 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
      241 tagcggatct tacctgacgc tttttatcgc aactctctac tgtttctcca tacccgctag
      301 caaagaggag aaaagaatgg aagcattagg aatgattgaa acccggggcc tggttgcgct
      361 gattgaggcc tccgatgcga tggtaaaagc cgcgcgcgtg aagctggtcg gcgtgaagca
      421 gattggcggt ggcctgtgta ctgccatggt gcgtggcgat gtggcggcgt gcaaagccgc
      481 aaccgatgct ggcgccgctg cggcgcagcg cattggcgag ttggtctccg tacacgtgat
      541 tccacgcccg cacggcgatc tggaagaagt gttcccgatc ggcttcaaag gcgacagcaa
      601 cattatggcg agtagcgaag acgttatcaa agagttcatg cgtttcaaag ttcgtatgga
      661 aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga
      721 aggtacccag accgctaaac tgaaagttac caaaggtggt ccgctgccgt tcgcttggga
      781 catcctgtcc ccgcagttcc agtacggttc caaagcttac gttaaacacc cggctgacat
      841 cccggactac ctgaaactgt cattcccgga aggtttcaaa tgggaacgtg ttatgaactt
      901 cgaagacggt ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg gtgagttcat
      961 ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac ggtacggtta tgcagaaaaa
     1021 aaccatgggt tgggaagctt ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg
     1081 tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac tacgacgctg aagttaaaac
     1141 cacctacatg gctaaaaaac cggttcagct gccgggtgct tacaaaaccg acatcaaact
     1201 ggacataacc tccaacaacg aagactacac catcgttgaa cagtacgaac gtgctgaagg
     1261 tcgtcactcc accggtgctt aataaggatc tccaggcatc aaataaaacg aaaggctcag
     1321 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaactaatt tctagactgc
     1381 aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcta gcggtattag
     1441 ctcactcaaa ggcggtaata cggttatcca cagaatcatg ggataacgca ggaaagaaca
     1501 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1561 tccacaggct ccgcccccct gactagcatc acaaaaatcg acgctcaagt cataggtggc
     1621 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1681 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1741 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1801 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1861 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1921 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1981 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2041 tcggaaaaag agtttgtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2101 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2161 tcttttctac tgggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2221 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2281 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     2341 cacctatctc agcgatctgt ctatttcgtt catccatagt tgccttactc cccgtcgtgt
     2401 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     2461 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     2521 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     2581 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     2641 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     2701 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     2761 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     2821 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     2881 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     2941 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     3001 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     3061 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     3121 gaaggcaaaa tgccgcaaaa aatggaataa gggcgacacg gaaatgttga atactcatac
     3181 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3241 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     3301 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     3361 tcactaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga
     3421 attcactagt ctatgctact ccatcgagcc gtcaattgtc tgattcgtta ccaattatga
     3481 caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct
     3541 ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt
     3601 gcgaccgacg gtggcgatag gcatccgggt tgtgctcaaa agcagcttcg cctggcttat
     3661 acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagacgtga
     3721 cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc
     3781 tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg
     3841 gagcgactcg ttaatcgctt ccatgcgccg cagcaacaat tgctcaagca gatttatcgc
     3901 cagcagctcc gaatagcgcc cttccccttg cccggcgtta attatttgcc caaacaggtc
     3961 gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga
     4021 cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc acttgtgata
     4081 ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggct ggaacagcaa
     4141 aatatcaccc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg
     4201 aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag
     4261 ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc
     4321 cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccgc cat
//
