LOCUS       Exported                8572 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Localizes CRY2 to excitatory synapses.
ACCESSION   .
VERSION     .
KEYWORDS    CRY2-GFP-homer1c
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Sinnen BL, Bowen AB, Forte JS, Hiester BG, Crosby KC, Gibson ES, 
            Dell'Acqua ML, Kennedy MJ
  TITLE     Optogenetic Control of Synaptic Composition and Function.
  JOURNAL   Neuron. 2017 Feb 8;93(3):646-660.e5. doi: 
            10.1016/j.neuron.2016.12.037. Epub 2017 Jan 26.
  PUBMED    28132827
REFERENCE   2  (bases 1 to 8572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(42..630)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(122..141)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(801..1661)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1424..1443
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1662..1766)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        1797..2176
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2178..2455
                     /label=chicken beta-actin promoter
     intron          2456..3468
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3392..3414
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3476..3495
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3535..5370
                     /codon_start=1
                     /product="Arabidopsis thaliana cryptochrome 2 in which the 
                     nuclear localization signal was removed by mutating 
                     residues 541 and 542 to alanine"
                     /label=CRY2(Delta-NLS)
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAAPDEIVADSFEALGANTIKE
                     PGLCPSVSSNDQQVPSAVRYNGSAAVKPEEEEERDMKKSRGFDERELFSTAESSSSSSV
                     FFVSQSCSLASEGKNLEGIQDSSDQITTSLGKNGCK"
     regulatory      5389..5398
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             5395..6111
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             5395..6111
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5440..5461)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5701..5720)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6048..6069
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7356..7375)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7421..7476
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7475..7494)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7867..7883)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7867..7883)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7880..7902)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7891..7907
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7915..7945)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7960..7981
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8040..8235
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8086..8221
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8148..8167
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    8241..8375
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8278..8297)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8332..8351
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8443..8460)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
       61 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      121 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      181 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      241 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      301 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      361 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      421 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      481 agracagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      541 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      601 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      661 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
      721 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
      781 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
      841 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
      901 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
      961 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     1021 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     1081 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     1141 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     1201 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     1261 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     1321 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     1381 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     1441 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     1501 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     1561 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     1621 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     1681 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     1741 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtcgaca
     1801 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     1861 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     1921 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     1981 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     2041 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     2101 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     2161 catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc
     2221 cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg
     2281 ggcggggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg
     2341 ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt
     2401 tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt
     2461 cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc cgccccggct
     2521 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg
     2581 taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg aaagccttaa
     2641 agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg tgcgtgtgtg
     2701 tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc gctgcgggcg
     2761 cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg gggcggtgcc
     2821 ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg
     2881 ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca cccccctccc
     2941 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg
     3001 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct
     3061 cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga
     3121 ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt
     3181 cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc
     3241 gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt
     3301 gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac
     3361 ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc
     3421 tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac
     3481 gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tgctcgaggc caccatgaag
     3541 atggacaaaa agactatagt ttggtttaga agagacctaa ggattgagga taatcctgca
     3601 ttagcagcag ctgctcacga aggatctgtt tttcctgtct tcatttggtg tcctgaagaa
     3661 gaaggacagt tttatcctgg aagagcttca agatggtgga tgaaacaatc acttgctcac
     3721 ttatctcaat ccttgaaggc tcttggatct gacctcactt taatcaaaac ccacaacacg
     3781 atttcagcga tcttggattg tatccgcgtt accggtgcta caaaagtcgt ctttaaccac
     3841 ctctatgatc ctgtttcgtt agttcgggac cataccgtaa aggagaagct ggtggaacgt
     3901 gggatctctg tgcaaagcta caatggagat ctattgtatg aaccgtggga gatatactgc
     3961 gaaaagggca aaccttttac gagtttcaat tcttactgga agaaatgctt agatatgtcg
     4021 attgaatccg ttatgcttcc tcctccttgg cggttgatgc caataactgc agcggctgaa
     4081 gcgatttggg cgtgttcgat tgaagaacta gggctggaga atgaggccga gaaaccgagc
     4141 aatgcgttgt taactagagc ttggtctcca ggatggagca atgctgataa gttactaaat
     4201 gagttcatcg agaagcagtt gatagattat gcaaagaaca gcaagaaagt tgttgggaat
     4261 tctacttcac tactttctcc gtatctccat ttcggggaaa taagcgtcag acacgttttc
     4321 cagtgtgccc ggatgaaaca aattatatgg gcaagagata agaacagtga aggagaagaa
     4381 agtgcagatc tttttcttag gggaatcggt ttaagagagt attctcggta tatatgtttc
     4441 aacttcccgt ttactcacga gcaatcgttg ttgagtcatc ttcggttttt cccttgggat
     4501 gctgatgttg ataagttcaa ggcctggaga caaggcagga ccggttatcc gttggtggat
     4561 gccggaatga gagagctttg ggctaccgga tggatgcata acagaataag agtgattgtt
     4621 tcaagctttg ctgtgaagtt tcttctcctt ccatggaaat ggggaatgaa gtatttctgg
     4681 gatacacttt tggatgctga tttggaatgt gacatccttg gctggcagta tatctctggg
     4741 agtatccccg atggccacga gcttgatcgc ttggacaatc ccgcgttaca aggcgccaaa
     4801 tatgacccag aaggtgagta cataaggcaa tggcttcccg agcttgcgag attgccaact
     4861 gaatggatcc atcatccatg ggacgctcct ttaaccgtac tcaaagcttc tggtgtggaa
     4921 ctcggaacaa actatgcgaa acccattgta gacatcgaca cagctcgtga gctactagct
     4981 aaagctattt caagaacccg tgaagcacag atcatgatcg gagcagcacc tgatgagatt
     5041 gtagcagata gcttcgaggc cttaggggct aataccatta aagaacctgg tctttgccca
     5101 tctgtgtctt ctaatgacca acaagtacct tcggctgttc gttacaacgg gtcagcggca
     5161 gtgaaacctg aggaagaaga agagagagac atgaagaaat ctaggggatt cgatgaaagg
     5221 gagttgtttt cgactgctga atcttcttct tcttcgagtg tgtttttcgt ttcgcagtct
     5281 tgctcgttgg catcagaagg gaagaatctg gaaggtattc aagattcatc tgatcagatt
     5341 actacaagtt tgggaaaaaa tggttgcaaa gcccgggatc caccggtcgc caccatggtg
     5401 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     5461 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     5521 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     5581 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     5641 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     5701 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     5761 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     5821 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     5881 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     5941 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     6001 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     6061 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa ggaattcggt
     6121 ggtggtggtg ggtcgaccat gggggaacaa cctatcttca gcactcgagc tcatgtcttc
     6181 cagatcgacc caaacacaaa gaagaactgg gtacccacca gcaagcatgc agttactgtg
     6241 tcttatttct atgacagcac aaggaatgtg tataggataa tcagtttaga cggctcaaag
     6301 gcaataataa atagcaccat cactccaaac atgacattta ctaaaacatc tcaaaagttt
     6361 ggccaatggg ctgatagccg ggcaaacact gtttatggac tgggattctc ctctgagcat
     6421 catctctcaa aatttgcaga aaagtttcag gaatttaaag aagctgctcg gctggcaaag
     6481 gagaagtcgc aggagaagat ggaactgacc agtacccctt cacaggaatc agcaggagga
     6541 gatcttcagt ctcctttaac accagaaagt atcaatggga cagatgatga gagaacaccc
     6601 gatgtgacac agaactcaga gccaagggct gagccagctc agaatgcatt gccattttca
     6661 catagtgccg gggatcgaac ccagggcctc tctcatgcta gttcagccat cagcaaacac
     6721 tgggaggctg aactagccac gctcaagggg aacaatgcca agctcaccgc agcgctgctg
     6781 gagtccactg ccaacgtgaa gcagtggaag caacagctgg ctgcctacca ggaggaggca
     6841 gagcggctgc acaagcgggt cacggagctg gaatgtgtta gtagtcaagc aaacgcggtg
     6901 cacagccaca agacagagct gagtcagaca gtgcaggagc tggaagagac cctaaaagta
     6961 aaggaagagg aaatagaaag attaaaacaa gaaattgata acgccagaga acttcaagaa
     7021 cagagggact ctttgactca gaaactacag gaagttgaga ttcgaaataa agacctggag
     7081 gggcagctgt cggagctgga gcagcgcctg gagaagagcc agagcgagca ggacgctttc
     7141 cgcagtaacc tgaagactct cctagagatt ctggacggga aaatatttga actaacagaa
     7201 ttgcgggata atttggccaa gctactagaa tgcagctaag aattctgcag atatccatca
     7261 cactggcggc cgcaattcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt
     7321 gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat
     7381 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
     7441 attgcaatag tgtgttggaa ttttttgtgt ctatcactcg gaaggacata tgggagggca
     7501 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
     7561 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct
     7621 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
     7681 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
     7741 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga
     7801 tccctcgacc tgcaccgtcg accagctggt cgacggtgca ccgtcgacca gcttggcgta
     7861 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7921 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     7981 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg
     8041 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     8101 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     8161 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     8221 ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac aaataaagca
     8281 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     8341 ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa tcggccaacg
     8401 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     8461 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     8521 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc ag
//
