LOCUS Exported 3854 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION codes for QF2 (truncated QF) in a gateway middle entry backbone.. ACCESSION . VERSION . KEYWORDS pME-QF2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3854) TITLE Halpern Lab QF2 gateway middle entry JOURNAL Unpublished REFERENCE 2 (bases 1 to 3854) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3854 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 8..30 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 22..39 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 23..39 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 55..154 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1243..1267) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" polyA_signal 1269..1403 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1293..1312) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 1347..1366 /label=SV40pA-R /note="SV40 polyA, reverse primer" protein_bind complement(1465..1564) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1581..1600) /label=T7 /note="T7 promoter, forward primer" promoter complement(1582..1600) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1605..1621) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1605..1621) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1723..1742) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1734..2543 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1801..1820) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2690..3278 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3179..3198 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3432..3449 /label=L4440 /note="L4440 vector, forward primer" terminator 3608..3635 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3727..3813 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccccaaata 61 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt 121 tttataatgc caactttgta caaaaaagca ggctcaacat gccacccaag cgcaaaacgc 181 ttaacgctgc ggctgaggct aacgctcatg ccgacggaca cgccgacgga aacgccgacg 241 gacacgtggc caatacggcc gcgtcctcga ataatgcgag gttcgctgat ctcactaaca 301 tcgatactcc gggtctggga cccacaacta cgaccctgct cgtggaacca gcacgctcaa 361 agcgtcaacg agtgtcccgc gcatgcgacc agtgccgtgc agcccgagag aaatgcgacg 421 gaatacagcc tgcgtgtttc ccgtgcgttt cccagggaag gtcctgcact tatcaggctt 481 cgccgaaaaa gaggggagtt caaaccggtt atattcgtac gctggagctc gccctcgcct 541 ggatgtttga aaatgtcgcg cgttccgaag atgccttgca taacctcctc gtccgtgacg 601 ccggacaagg atcagctctg ctcgttggta aagattcgcc ggctgccgag cgactccatg 661 cccgttgggc tactagccgt gtcaataaga gcattacccg cctcctccgt cagttggagc 721 tccctcctac cgccacggct acggcctcga taatgccgca cgtgatggag cagcctctca 781 gtaccagcat taaccccgtc aacgaccgct tcaacggtat tcccaacccc actccgtata 841 actccgatgc agctctcgat gctatcactc agaccaacga ttatggaagc gtaaatacac 901 atggtatcct ctctacttac ccgccaccgg ctacgcacct taatgaagct tccgtcgctc 961 tcgctcccgg tggcgccccc ccccgaccgc ctcctccgta tgttgacagc acgaccaatc 1021 acccgccgta ccactcgaat ctggttccaa tggcgaactt tggttactcg accgttgatt 1081 acgatgccat ggttgacgat ttggctagca ttgaatacac ggacgctgtg gatgtcgacc 1141 cacagtttat gaccaatctg ggattcgttc ctggatgtaa cttctccgac attaatacat 1201 acgaacagtg atgacgtcta tcgataccgt cgactaaagc caacccagct ttcttgtaca 1261 aagtggggga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa 1321 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca 1381 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc 1441 agggggaggt gtgggaggtt ttttacccag ctttcttgta caaagttggc attataagaa 1501 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta 1561 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct 1621 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat 1681 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc 1741 atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg 1801 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg 1861 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg 1921 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca 1981 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa 2041 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg 2101 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc 2161 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg 2221 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac 2281 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta 2341 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc 2401 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga 2461 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt 2521 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca 2581 ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg 2641 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 2701 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 2761 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 2821 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 2881 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 2941 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 3001 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 3061 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 3121 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 3181 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 3241 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 3301 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg 3361 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa 3421 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc 3481 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 3541 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag 3601 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta 3661 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc 3721 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc 3781 ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcgt 3841 taacgctagc atgg //