LOCUS Exported 2143 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Type 2, pGAP, Glyceraldehyde-3-phosphate dehydrogenase promoter. ACCESSION . VERSION . KEYWORDS pPTK002-2-pGAP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2143) AUTHORS Obst U, Lu TK, Sieber V TITLE A Modular Toolkit for Generating Pichia pastoris Secretion Libraries. JOURNAL ACS Synth Biol. 2017 Mar 15. doi: 10.1021/acssynbio.6b00337. PUBMED 28252957 REFERENCE 2 (bases 1 to 2143) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2143 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..477 /label=GAP promoter /note="promoter for Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase" terminator 495..529 /label=lambda t0 terminator /note="minimal transcription terminator from phage lambda (Scholtissek and Grosse, 1987)" CDS complement(600..1259) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 1174..1193 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" rep_origin 1441..2029 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1930..1949 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tttttgtaga aatgtcttgg tgtcctcgtc caatcaggta gccatctctg aaatatctgg 61 ctccgttgca actccgaacg acctgctggc aacgtaaaat tctccggggt aaaacttaaa 121 tgtggagtaa tggaaccaga aacgtgtctt cccttctctc tccttccacc gcccgttacc 181 gtccctagga aattttactc tgctggagag cttcttctac ggcccccttg cagcaatgct 241 cttcccagca ttacgttgcg ggtaaaacgg aggtcgtgta cccgacctag cagcccaggg 301 atggaaaagt cccggccgtc gctggcaata atagcgggcg gacgcatgtc atgagattat 361 tggaaaccac cagaatcgaa tataaaaggc gaacaccttt cccaattttg gtttctcctg 421 acccaaagac tttaaattta atttatttgt ccctatttca atcaattgaa caactattat 481 gtgagaccag accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat 541 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat 601 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca 661 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg 721 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc 781 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgaaacgaa aaacatattc 841 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa 901 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt 961 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca 1021 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga 1081 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata 1141 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt 1201 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt 1261 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt 1321 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga ccaaaatccc 1381 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 1441 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 1501 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 1561 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 1621 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 1681 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 1741 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 1801 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 1861 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 1921 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 1981 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 2041 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 2101 gttatcccct gattctgtgg ataaccgtag tcggtctcaa acg //