LOCUS       Exported                1890 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Type 3a, Glucoamylase followed by alpha-MF-Delta - secretion signal.
ACCESSION   .
VERSION     .
KEYWORDS    pPTK010-3a-Glucoamylase-alpha-MF-Delta
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Obst U, Lu TK, Sieber V
  TITLE     A Modular Toolkit for Generating Pichia pastoris Secretion 
            Libraries.
  JOURNAL   ACS Synth Biol. 2017 Mar 15. doi: 10.1021/acssynbio.6b00337.
  PUBMED    28252957
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      245..279
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(350..1009)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     924..943
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1191..1779
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1680..1699
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgtctttca gatccctatt ggcattgtca gggttggtct gttctggatt ggctatgaga
       61 tttcctagta ttttcactgc tgtgctattt gccgctagtt ccgctctagc tgctccagtt
      121 aatactacta ctgaagatga attggagggt gacttcgatg ttgctgttct gcctttttcc
      181 gcttctatcg cagccaagga agaaggtgta tctctagaga agcgtggttc ttgagaccag
      241 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
      301 ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc
      361 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
      421 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
      481 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
      541 caaaactggt gaaactcacc cagggattgg ctgaaacgaa aaacatattc tcaataaacc
      601 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
      661 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
      721 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
      781 ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
      841 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
      901 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
      961 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
     1021 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
     1081 ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga ccaaaatccc ttaacgtgag
     1141 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     1201 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     1261 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     1321 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     1381 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     1441 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     1501 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     1561 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     1621 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     1681 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     1741 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     1801 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     1861 gattctgtgg ataaccgtag tcggtctcat
//
