LOCUS Exported 2403 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Type 3, Red fluorescent protein. ACCESSION . VERSION . KEYWORDS pPTK016-3-RFP_Intra SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2403) AUTHORS Obst U, Lu TK, Sieber V TITLE A Modular Toolkit for Generating Pichia pastoris Secretion Libraries. JOURNAL ACS Synth Biol. 2017 Mar 15. doi: 10.1021/acssynbio.6b00337. PUBMED 28252957 REFERENCE 2 (bases 1 to 2403) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2403 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 755..789 /label=lambda t0 terminator /note="minimal transcription terminator from phage lambda (Scholtissek and Grosse, 1987)" CDS complement(860..1519) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 1434..1453 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" rep_origin 1701..2289 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2190..2209 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gcaacttccg gtatggtgtc aaagggagag gaaaataata tggctattat taaggagttt 61 atgcgtttta aggtacatat ggaaggttct gtcaacggtc acgaattcga aattgaaggt 121 gagggggagg ggaggccata cgagggaact cagactgcta agttaaaggt cactaaaggt 181 ggtcctttac ctttcgcctg ggatatcctg tctccacagt ttatgtacgg ttcaaaggct 241 tatgtgaaac atcctgccga tatcccagat tatcttaaac tttctttccc tgagggtttt 301 aagtgggaga gggtaatgaa ctttgaagac ggtggtgtgg tcactgttac tcaggactca 361 agtctgcagg acggtgagtt catctacaag gtgaagctga gaggtaccaa ttttccatca 421 gatggtcccg tgatgcaaaa aaagacaatg ggttgggaag cttctagtga acgtatgtat 481 cccgaagatg gagctttgaa aggtgaaatt aagcaaagac taaaacttaa ggatggtgga 541 cattacgatg ctgaagttaa gacgacctac aaggccaaaa agccagtcca gttgcctgga 601 gcatacaatg ttaacatcaa attggatata acttcccata atgaagacta taccatcgtc 661 gagcaatacg aacgagccga agggagacac agtactggtg gtatggatga actttataaa 721 ggatccggaa ccgcaggatc ctgagaccag accaataaaa aacgcccggc ggcaaccgag 781 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc 841 aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt 901 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca 961 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga 1021 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg 1081 ctgaaacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt 1141 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac 1201 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac 1261 tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca 1321 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg 1381 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg 1441 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc 1501 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa 1561 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga 1621 tcaatcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 1681 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 1741 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 1801 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 1861 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 1921 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 1981 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 2041 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 2101 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 2161 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 2221 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 2281 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 2341 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtag tcggtctcat 2401 atg //