LOCUS Exported 10705 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Expresses FRB-tagged human Akt1(D274A) kinase-inactive mutant. ACCESSION . VERSION . KEYWORDS pHR-FRB:Akt1(D274A) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10705) AUTHORS Ebner M, Lucic I, Leonard TA, Yudushkin I TITLE PI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. JOURNAL Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. PUBMED 28157504 REFERENCE 2 (bases 1 to 10705) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10705 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 45..633 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 534..553 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 787..804 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(874..894) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 879..1208 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 1059..1194 /label=SV40 ori /note="SV40 origin of replication" primer_bind 1121..1140 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" intron 2342..2407 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 2537..2557 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 2982..3116 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3019..3038) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3073..3092 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 3284..3917 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 3964..4089 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 4586..4819 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 5004..5048 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 5333..5450 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 5603..6010 /label=SFFV promoter /note="spleen focus-forming virus long terminal repeat (LTR) promoter" CDS 6053..6331 /codon_start=1 /product="T2098L mutant of FKBP-rapamycin binding domain of human FRAP" /label=FRB* /note="binds synthetic ligands such as AP21967" /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK" CDS 6344..7780 /codon_start=1 /gene="AKT1" /product="human serine/threonine protein kinase activated by growth factors" /label=Akt1 /translation="MSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDV DQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQ TVADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGK VILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDR LCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRALKLENLM LDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMY EMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDA KEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSM ECVDSERRPHFPQFSYSASGT" misc_feature 7852..8440 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(7905..7925) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8323..8334) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(8443..8459) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8444..8460) /label=pBluescriptKS /note="For pBluescript vector" LTR 8550..8783 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" promoter complement(8808..8826) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(8809..8826) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(9116..9135) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind complement(9349..9368) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 9468..9490 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(9528..9546) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 9614..9718 /gene="bla" /label=AmpR promoter CDS 9719..10579 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9937..9956) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" ORIGIN 1 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 61 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 121 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 181 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 241 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 301 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 361 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 421 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 481 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 541 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 601 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 661 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 721 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 781 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc 841 cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa 901 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 961 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 1021 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 1081 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 1141 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 1201 tttgcaaaaa gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat 1261 cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt 1321 agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc 1381 gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg 1441 ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc 1501 attattgccg taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta 1561 ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta 1621 aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg 1681 ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca 1741 tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag 1801 atacctggat tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc 1861 gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta 1921 caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa 1981 accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat 2041 cacggcgcac aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat 2101 cgcatgatta accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac 2161 gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg 2221 gtgattggct accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga 2281 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa 2341 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt 2401 attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga 2461 ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc 2521 tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc 2581 agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc 2641 tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc 2701 tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc 2761 acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt 2821 tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca 2881 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc 2941 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt 3001 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 3061 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact 3121 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg 3181 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt 3241 aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac 3301 tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct 3361 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc 3421 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc 3481 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag 3541 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac 3601 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg 3661 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct 3721 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg 3781 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt 3841 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 3901 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag 3961 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg 4021 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc 4081 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg 4141 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa 4201 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga 4261 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta 4321 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac 4381 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga 4441 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata 4501 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc 4561 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag 4621 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg 4681 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc 4741 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc 4801 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg 4861 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga 4921 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg 4981 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg 5041 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa 5101 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata 5161 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac 5221 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc 5281 gattagtgaa cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa 5341 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag 5401 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt 5461 acagggacag cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc 5521 gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg 5581 taggtttggc aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc 5641 aagaatagag aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac 5701 aggatatctg cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc 5761 agtttcggcc ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag 5821 cagtttctta agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc 5881 gccttatttg aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc 5941 cgagctctat aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga 6001 gtcgcccggg ggggatctgg agctctcgag aattctcacg cgcgccacca tgatcctctg 6061 gcatgagatg tggcatgaag gcctggaaga ggcatctcgt ttgtactttg gggaaaggaa 6121 cgtgaaaggc atgtttgagg tgctggagcc cttgcatgct atgatggaac ggggccccca 6181 gactctgaag gaaacatcct ttaatcaggc ctatggtcga gatttaatgg aggcccaaga 6241 gtggtgcagg aagtacatga aatcagggaa tgtcaaggac ctcctccaag cctgggacct 6301 ctattatcat gtgttccgac gaatctcaaa gacgcgtgcc accatgagcg acgtggctat 6361 tgtgaaggag ggttggctgc acaaacgagg ggagtacatc aagacctggc ggccacgcta 6421 cttcctcctc aagaatgatg gcaccttcat tggctacaag gagcggccgc aggatgtgga 6481 ccaacgtgag gctcccctca acaacttctc tgtggcgcag tgccagctga tgaagacgga 6541 gcggccccgg cccaacacct tcatcatccg ctgcctgcag tggaccactg tcatcgaacg 6601 caccttccat gtggagactc ctgaggagcg ggaggagtgg acaaccgcca tccagactgt 6661 ggctgacggc ctcaagaagc aggaggagga ggagatggac ttccggtcgg gctcacccag 6721 tgacaactca ggggctgaag agatggaggt gtccctggcc aagcccaagc accgcgtgac 6781 catgaacgag tttgagtacc tgaagctgct gggcaagggc actttcggca aggtgatcct 6841 ggtgaaggag aaggccacag gccgctacta cgccatgaag atcctcaaga aggaagtcat 6901 cgtggccaag gacgaggtgg cccacacact caccgagaac cgcgtcctgc agaactccag 6961 gcaccccttc ctcacagccc tgaagtactc tttccagacc cacgaccgcc tctgctttgt 7021 catggagtac gccaacgggg gcgagctgtt cttccacctg tcccgggagc gtgtgttctc 7081 cgaggaccgg gcccgcttct atggcgctga gattgtgtca gccctggact acctgcactc 7141 ggagaagaac gtggtgtacc gggccctcaa gctggagaac ctcatgctgg acaaggacgg 7201 gcacattaag atcacagact tcgggctgtg caaggagggg atcaaggacg gtgccaccat 7261 gaagaccttt tgcggcacac ctgagtacct ggcccccgag gtgctggagg acaatgacta 7321 cggccgtgca gtggactggt gggggctggg cgtggtcatg tacgagatga tgtgcggtcg 7381 cctgcccttc tacaaccagg accatgagaa gctttttgag ctcatcctca tggaggagat 7441 ccgcttcccg cgcacgcttg gtcccgaggc caagtccttg ctttcagggc tgctcaagaa 7501 ggaccccaag cagaggcttg gcgggggctc cgaggacgcc aaggagatca tgcagcatcg 7561 cttctttgcc ggtatcgtgt ggcagcacgt gtacgagaag aagctcagcc cacccttcaa 7621 gccccaggtc acgtcggaga ctgacaccag gtattttgat gaggagttca cggcccagat 7681 gatcaccatc acaccacctg accaagatga cagcatggag tgtgtggaca gcgagcgcag 7741 gccccacttc ccccagttct cctactcggc cagcggcacg gcggatccat gagcggccgc 7801 gactctagag tcgacctgca ggcatgcaag cttgatatca agcttatcga taatcaacct 7861 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 7921 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 7981 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 8041 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 8101 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 8161 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 8221 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt 8281 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 8341 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc 8401 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga 8461 gggaattaat tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct 8521 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca 8581 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct 8641 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 8701 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 8761 gtcagtgtgg aaaatctcta gcagcatcta gaattaattc cgtgtattct atagtgtcac 8821 ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg ttctaacgac 8881 aatatgtaca agcctaattg tgtagcatct ggcttactga agcagaccct atcatctctc 8941 tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct tctgagtttc tggtaacgcc 9001 gtcccgcacc cggaaatggt cagcgaacca atcagcaggg tcatcgctag ccagatcctc 9061 tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat 9121 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt 9181 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg 9241 catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc 9301 ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta caatctgctc 9361 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg 9421 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat 9481 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg 9541 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt 9601 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 9661 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 9721 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 9781 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 9841 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 9901 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 9961 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 10021 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 10081 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 10141 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 10201 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 10261 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 10321 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 10381 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 10441 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 10501 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 10561 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 10621 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 10681 caaaatccct taacgtgagt tttcg //