LOCUS       Exported               10286 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses PP7-2xmCherry-CAAX.
ACCESSION   .
VERSION     .
KEYWORDS    pHR-PP7-2xmCherry-CAAX
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10286)
  AUTHORS   Yan X, Hoek TA, Vale RD, Tanenbaum ME
  TITLE     Dynamics of Translation of Single mRNA Molecules In Vivo.
  JOURNAL   Cell. 2016 May 5;165(4):976-89. doi: 10.1016/j.cell.2016.04.034.
  PUBMED    27153498
REFERENCE   2  (bases 1 to 10286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10286
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(302..321)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     655..677
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(715..733)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        801..905
                     /gene="bla"
                     /label=AmpR promoter
     CDS             906..1766
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1124..1143)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1937..2525
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2426..2445
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2679..2696
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2766..2786)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2771..3100
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2951..3086
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4234..4299
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4429..4449
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4874..5008
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4911..4930)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4965..4984
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5177..5810
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5857..5982
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6474..6707
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6892..6936
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7221..7338
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7401..7611
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7470..7605
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8152..8856
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     CDS             8902..9063
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             9121..9825
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     LTR             10022..10255
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(10280..12)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(10281..12)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct
       61 aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca gaccctatca
      121 tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg agtttctggt
      181 aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat cgctagccag
      241 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
      301 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
      361 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
      421 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
      481 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat
      541 ctagctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
      601 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
      661 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
      721 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
      781 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      841 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      901 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      961 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     1021 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     1081 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     1141 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     1201 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     1261 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     1321 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     1381 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     1441 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     1501 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     1561 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     1621 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     1681 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     1741 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1801 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1861 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1921 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1981 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2041 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     2101 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2161 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2221 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2281 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2341 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2401 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2461 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2521 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     2581 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     2641 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     2701 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     2761 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     2821 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     2881 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     2941 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     3001 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     3061 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg
     3121 cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa
     3181 aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc
     3241 aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca
     3301 tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa
     3361 gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt
     3421 accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
     3481 cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg
     3541 cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg
     3601 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct
     3661 ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat
     3721 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc
     3781 gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg
     3841 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
     3901 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
     3961 cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
     4021 ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg
     4081 ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga
     4141 tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
     4201 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
     4261 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat
     4321 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
     4381 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag
     4441 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct
     4501 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca
     4561 ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt
     4621 tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat
     4681 aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa
     4741 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
     4801 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     4861 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     4921 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     4981 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
     5041 caaacttccc accccatacc ctattaccac tgccaattac ctagtggttt catttactct
     5101 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
     5161 ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat
     5221 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     5281 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     5341 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     5401 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     5461 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     5521 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     5581 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     5641 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     5701 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     5761 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     5821 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     5881 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     5941 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     6001 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     6061 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     6121 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     6181 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     6241 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     6301 aagaccaccg cacagcaagc ggccggtgat cttcagacct ggacgatata tatgagggac
     6361 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     6421 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     6481 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     6541 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     6601 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     6661 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     6721 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     6781 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     6841 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     6901 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     6961 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     7021 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     7081 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     7141 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgccaa
     7201 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     7261 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     7321 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggatcgat
     7381 aagcttgata tcgaattcag gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     7441 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     7501 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     7561 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc tacgcgccag
     7621 tcctccgaca gactgagtcg cccggggggg atctggagct ctcgagaatt ctcacgcgcc
     7681 ggatccgcca ccatgtccaa aaccatcgtt ctttcggtcg gcgaggctac tcgcactctg
     7741 actgagatcc agtccaccgc agaccgtcag atcttcgaag agaaggtcgg gcctctggtg
     7801 ggtcggctgc gcctcacggc ttcgctccgt caaaacggag ccaagaccgc gtatcgcgtc
     7861 aacctaaaac tggatcaggc ggacgtcgtt gattgctcca ccagcgtctg cggcgagctt
     7921 ccgaaagtgc gctacactca ggtatggtcg cacgacgtga caatcgttgc gaatagcacc
     7981 gaggcctcgc gcaaatcgtt gtacgatttg accaagtccc tcgtcgcgac ctcgcaggtc
     8041 gaagatcttg tcgtcaacct tgtgccgctg ggccgtcgtg cggacccgct agcctcctgc
     8101 ggccgcggtg gtggtacgcg tgattcgact agtggaggaa gcggaggagg agtgagcaag
     8161 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     8221 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     8281 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     8341 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     8401 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     8461 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     8521 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     8581 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     8641 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     8701 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     8761 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     8821 cgccactcca ccggcggcat ggacgagctg tacaagcatg gcatggatga gctctacaaa
     8881 ggtggaggtc ggaccgaaga gtacaagctt atcctgaacg gtaaaaccct gaaaggtgaa
     8941 accaccaccg aagctgttga cgctgctacc gcggaaaaag ttttcaaaca gtacgctaac
     9001 gacaacggtg ttgacggtga atggacctac gacgacgcta ccaaaacctt cacggtaacc
     9061 gaagctagtg gtggtggtgg tagcggtggt ggtagcagct taccggtcgc cgaattcggt
     9121 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
     9181 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     9241 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     9301 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
     9361 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     9421 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     9481 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     9541 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     9601 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     9661 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     9721 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     9781 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagcatat gggtggaggt
     9841 tctggtggtg gatctggtgg aggttctgga ggtggaaaaa tgtccaagga tggtaagaaa
     9901 aagaagaaga agtcaaaaac caagtgtgtt atcatgtaac atatggcggc cgtaccttta
     9961 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
    10021 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
    10081 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
    10141 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
    10201 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagcatc
    10261 tagaattaat tccgtgtatt ctatag
//
