LOCUS Exported 5257 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: Membrane, Excitation: 462, Emission: 492. ACCESSION . VERSION . KEYWORDS mTFP1-Caveolin-C-10 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5257) AUTHORS Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE TITLE Hue-shifted monomeric variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging. JOURNAL BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13. PUBMED 18325109 REFERENCE 2 (bases 1 to 5257) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5257 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1..708 /codon_start=1 /product="monomeric teal (cyan) fluorescent protein (Ai et al., 2006)" /label=mTFP1 /note="mammalian codon-optimized" /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA VARNSTDGMDELYK" polyA_signal 1433..1554 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1470..1489) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1524..1543 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1561..2016) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1698..1719) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 1910..1929 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2043..2147 /gene="bla" /label=AmpR promoter promoter 2149..2506 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2172..2192) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2357..2492 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2419..2438 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2541..3335 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2595..2614) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3205..3224 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3523..3542) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3567..3614 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 3943..4531 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4432..4451 /label=pBR322ori-F /note="pBR322 origin, forward primer" enhancer 4706..5009 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 5010..5213 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 5164..5184 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 5252..4 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 atggtgagca agggcgagga gaccacaatg ggcgtaatca agcccgacat gaagatcaag 61 ctgaagatgg agggcaacgt gaatggccac gccttcgtga tcgagggcga gggcgagggc 121 aagccctacg acggcaccaa caccatcaac ctggaggtga aggagggagc ccccctgccc 181 ttctcctacg acattctgac caccgcgttc gcctacggca acagggcctt caccaagtac 241 cccgacgaca tccccaacta cttcaagcag tccttccccg agggctactc ttgggagcgc 301 accatgacct tcgaggacaa gggcatcgtg aaggtgaagt ccgacatctc catggaggag 361 gactccttca tctacgagat acacctcaag ggcgagaact tcccccccaa cggccccgtg 421 atgcagaaga agaccaccgg ctgggacgcc tccaccgaga ggatgtacgt gcgcgacggc 481 gtgctgaagg gcgacgtcaa gcacaagctg ctgctggagg gcggcggcca ccaccgcgtt 541 gacttcaaga ccatctacag ggccaagaag gcggtgaagc tgcccgacta tcactttgtg 601 gaccaccgca tcgagatcct gaaccacgac aaggactaca acaaggtgac cgtttacgag 661 agcgccgtgg cccgcaactc caccgacggc atggacgagc tgtacaagtc cggactcaga 721 tctggcagca gtggaggttc tgggggcaaa tacgtagact cggagggaca tctctacacc 781 gttcccatcc gggaacaggg caacatctac aagcccaaca acaaggccat ggcagacgag 841 ctgagcgaga agcaagtgta cgacgcgcac accaaggaga tcgacctggt caaccgcgac 901 cctaaacacc tcaacgatga cgtggtcaag attgactttg aagatgtgat tgcagaacca 961 gaagggacac acagttttga cggcatttgg aaggccagct tcaccacctt cactgtgacg 1021 aaatactggt tttaccgctt gctgtctgcc ctctttggca tcccgatggc actcatctgg 1081 ggcatttact tcgccattct ctctttcctg cacatctggg cagttgtacc atgcattaag 1141 agcttcctga ttgagattca gtgcatcagc cgtgtctatt ccatctacgt ccacaccgtc 1201 tgtgacccac tctttgaagc tgttgggaaa atattcagca atgtccgcat caacttgcag 1261 aaagaaatat aagaattctg cagtcgacgg taccgcgggc ccgggatcca ccggatctag 1321 ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct 1381 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt 1441 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 1501 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt 1561 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat 1621 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga 1681 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca 1741 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct 1801 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc 1861 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag 1921 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca 1981 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg 2041 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 2101 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa 2161 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 2221 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 2281 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 2341 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 2401 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 2461 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga 2521 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg 2581 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg 2641 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt 2701 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg 2761 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat 2821 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat 2881 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg 2941 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg 3001 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc 3061 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc 3121 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg 3181 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg 3241 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca 3301 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac 3361 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga 3421 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga 3481 tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac 3541 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt 3601 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc 3661 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc 3721 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc 3781 ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 3841 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 3901 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 3961 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 4021 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 4081 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 4141 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 4201 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 4261 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 4321 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 4381 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 4441 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 4501 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 4561 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 4621 ggataaccgt attaccgcca tgcattagtt attaatagta atcaattacg gggtcattag 4681 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct 4741 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc 4801 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg 4861 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 4921 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 4981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 5041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 5101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 5161 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag 5221 tgaaccgtca gatccgctag cgctaccggt cgccacc //