LOCUS Exported 4579 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter. ACCESSION . VERSION . KEYWORDS pSTAP-seq_fly-zfh1_spikeIn-3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4579) AUTHORS Arnold CD, Zabidi MA, Pagani M, Rath M, Schernhuber K, Kazmar T, Stark A TITLE Genome-wide assessment of sequence-intrinsic enhancer responsiveness at single-base-pair resolution. JOURNAL Nat Biotechnol. 2016 Dec 26. doi: 10.1038/nbt.3739. PUBMED 28024147 REFERENCE 2 (bases 1 to 4579) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4579 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 1..456 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(88..107) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 298..319 /label=F1ori-F /note="F1 origin, forward primer" polyA_signal 587..635 /note="synthetic polyadenylation signal" misc_feature 649..740 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" primer_bind 689..708 /label=RVprimer3 /note="pGL3 vector, forward primer" regulatory 1952..1961 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" intron 1967..2033 /label=Mhc intron /note="modified Drosophila myosin heavy chain intron 16 (Pfeiffer et al., 2010)" primer_bind complement(2078..2106) /label=GFP-R /note="GFP, reverse primer. Does NOT anneal to EGFP" polyA_signal 2273..2394 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2284..2303) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 2338..2357 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(2658..2675) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2829..3417) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2909..2928) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3588..4448) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4211..4230 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(4449..4553) /gene="bla" /label=AmpR promoter ORIGIN 1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 421 acgcgaattt taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca 481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat 541 gataagtaag taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt 601 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct 661 ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc 721 aggtgccaga acatttctct atcgataggt accttaaaag tgccagtgct tgtggcgtgc 781 acggagaaaa aaaccttgag gtcatatttg aaaaaaaaaa aaaaaaaact aaattccaag 841 tgagtagttt actcttagtc actatcgagt gtgtgctgtt tgtttttcga aatatgcata 901 tcttagctac ttgcgttgct gttttttttt tttttttttt tttgacgatt tgtcaacagg 961 aaatatgcat aaggttggct cgggatccgg atcggatggc atggggtttg attggatgcc 1021 gtacttggca ccgcactgcc gttatcggtt ggctcatttc ccgttagccg cgaacaaagc 1081 ccacgcattg tgctacgacc gccgtgatgt gttcaaagat caagtgcccc cagaaccatc 1141 tcctgccatc cctgccatcc ctgcccgcct gcatccatcg cgggcattca gattccgact 1201 gcgcatgagc agcggtctcc aacacgtaag caagtggaag gcgagggata agaacaagta 1261 acttctctct tatcggacgc ttccaggaat ttcgatcctc gcaccgcatt ccgcaggttt 1321 agtcactgga ttccacggct tttcgagtcg attgtcccaa gaaacattat tttggacatg 1381 tgactcactg actcaaagct attatcactt ctcggacata tgcgtgtata taaaatattt 1441 ttaaacatta ttaggtatac tataaactct gcctcattca tgcctacaga acttcagcct 1501 tgacattcta tatatagaaa cccttactac gtctttactc tttaaatagt tctttggata 1561 ttatattagc acattgatgt gctttttttt tggatggttg tgtccaactc acggtaccga 1621 gctcttacgc gtgctagccc gggctcgaga tcttagagca tgcaccggac actctttccc 1681 tacacgacgc tcttccgatc ttttgtgcga gctttcgtcg attttggagc gaagcagcag 1741 ccccaacgcg tttggcgacg ccaacctgct tacagtttta gttgggagct tgcactgttc 1801 ggacgttcaa aagcccacaa gccacgcccc caccgacaag gcactggcaa aacacgcgac 1861 aaggcagcaa cagatcggaa gagcacacgt ctgaactcca gtcactcgac gaattcggcc 1921 cctgcagggg cattccggta ctgttggtaa agccaccatg gaaaaggtag gttcaaccac 1981 tgatgcctag gcacaccgaa acgactaacc ctaattctta tcctttactt caggccactt 2041 aagcatggct agcaaaggag aagaactctt cactggagtt gtcccaattc ttgttgaatt 2101 agatggtgat gttaacggcc acaagttctc tgtcagtgga gagggtgaag gtgatgcaac 2161 atacggaaaa cttaccctga agttcatctg cactactggc aaactgcctg ttccctggcc 2221 aacactagtc tgatctagag tcggggcggc cggccgcttc gagcagacat gataagatac 2281 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa 2341 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac 2401 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc 2461 aagtaaaacc tctacaaatg tggtaaaatc gataaggatc cgaccgatgc cctcaagctt 2521 tgagaggggc ccccttcaac ccagtcagct ccgacgtctt ggcgcccccg cggggcatga 2581 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg 2641 cagcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 2701 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 2761 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 2821 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 2881 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 2941 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 3001 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 3061 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 3121 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 3181 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 3241 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag 3301 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 3361 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 3421 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 3481 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 3541 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 3601 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 3661 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 3721 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 3781 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 3841 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 3901 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 3961 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 4021 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 4081 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 4141 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 4201 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 4261 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 4321 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 4381 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 4441 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 4501 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 4561 ccccgaaaag tgccacctg //