LOCUS Exported 8815 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Tet inducible scrambled shRNA.. ACCESSION . VERSION . KEYWORDS Tet-pLKO-puro-Scrambled SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8815) AUTHORS Rudin CM, Durinck S, Stawiski EW, Poirier JT, Modrusan Z, Shames DS, Bergbower EA, Guan Y, Shin J, Guillory J, Rivers CS, Foo CK, Bhatt D, Stinson J, Gnad F, Haverty PM, Gentleman R, Chaudhuri S, Janakiraman V, Jaiswal BS, Parikh C, Yuan W, Zhang Z, Koeppen H, Wu TD, Stern HM, Yauch RL, Huffman KE, Paskulin DD, Illei PB, Varella-Garcia M, Gazdar AF, de Sauvage FJ, Bourgon R, Minna JD, Brock MV, Seshagiri S TITLE Comprehensive genomic analysis identifies SOX2 as a frequently amplified gene in small-cell lung cancer. JOURNAL Nat Genet. 2012 Oct;44(10):1111-6. doi: 10.1038/ng.2405. Epub 2012 Sep 2. PUBMED 22941189 REFERENCE 2 (bases 1 to 8815) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8815 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 258..278 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 489..722 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 794..915 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(831..850) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 885..904 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 955..1090 /label=SV40 ori /note="SV40 origin of replication" primer_bind 1017..1036 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter complement(1111..1129) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1139..1156) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(1139..1155) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1148..1170) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 1297..1752 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1384..1403) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 1594..1615 /label=F1ori-F /note="F1 origin, forward primer" promoter 1778..1882 /gene="bla" /label=AmpR promoter CDS 1883..2743 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(2101..2120) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 2914..3502 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3403..3422 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3656..3673 /label=L4440 /note="L4440 vector, forward primer" protein_bind 3790..3811 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 3826..3856 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3864..3880 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 3869..3891 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 3888..3904 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 3888..3904 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 3923..3943 /label=T3 /note="T3 promoter, forward primer" promoter 3925..3943 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 3971..4197 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 4198..4378 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 4425..4550 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 5043..5276 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 5461..5505 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 5794..5888 /label=H1-2O2 promoter /note="doxycycline-inducible variant of the human H1 RNA promoter (Henriksen et al., 2007)" primer_bind 5805..5824 /label=H1 /note="Human H1 promoter, forward primer" protein_bind 5842..5860 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 5870..5888 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" misc_feature 5988..6105 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 6154..6664 /label=hPGK promoter /note="human phosphoglycerate kinase 1 promoter" primer_bind 6583..6601 /label=hPGK-F /note="Human PGK promoter, forward primer" intron 6673..7245 /label=-beta-globin intron /note="intron from rabbit beta-globin gene" primer_bind 7253..7272 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" promoter 7300..7318 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 7329..7946 /codon_start=1 /gene="tetR from transposon Tn10" /product="tetracycline repressor TetR" /label=TetR /note="TetR binds to the tetracycline operator tetO to inhibit transcription. This inhibition can be relieved by adding tetracycline or doxycycline." /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESG" primer_bind complement(7402..7421) /label=Tet-R /note="Tetracycline resistance gene, reverse primer" misc_feature 7984..8557 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(8151..8168) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 8378..8397 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 8577..361 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(8577..8596) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" ORIGIN 1 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg 61 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga 121 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg 181 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct 241 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc 301 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg 361 acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatcggt 421 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 481 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac 541 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 601 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 661 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 721 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 781 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 841 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 901 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg 961 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 1021 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 1081 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac 1141 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc 1201 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc 1261 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa 1321 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 1381 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 1441 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 1501 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 1561 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 1621 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct 1681 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa 1741 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 1801 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 1861 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 1921 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 1981 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 2041 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 2101 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 2161 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 2221 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 2281 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 2341 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 2401 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 2461 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 2521 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 2581 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 2641 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 2701 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 2761 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 2821 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 2881 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 2941 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 3001 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 3061 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 3121 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 3181 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 3241 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 3301 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 3361 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 3421 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 3481 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 3541 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 3601 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga 3661 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 3721 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 3781 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct 3841 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta 3901 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct 3961 gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt 4021 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg 4081 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc 4141 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg 4201 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg 4261 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt 4321 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt 4381 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga 4441 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa 4501 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc 4561 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat 4621 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg 4681 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc 4741 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc 4801 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa 4861 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat 4921 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta 4981 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga 5041 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca 5101 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat 5161 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag 5221 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg 5281 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg 5341 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa 5401 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa 5461 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac 5521 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt 5581 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca 5641 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa 5701 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg 5761 tatcgatcac gagactagcc tcgagcggcc gcaatatttg catgtcgcta tgtgttctgg 5821 gaaatcacca taaacgtgaa atccctatca gtgatagaga cttataagtt ccctatcagt 5881 gatagagaca ccggcctaag gttaagtcgc cctcgctcga gcgagggcga cttaacctta 5941 ggtttttaat tctcgacctc gagacaaatg gcagtattca tccacaattt taaaagaaaa 6001 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata 6061 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg 6121 gacagcagag atccactttg gccgcggctc gagggggttg gggttgcgcc ttttccaagg 6181 cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg 6241 cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc 6301 cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc 6361 cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct 6421 cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa 6481 tagcggctgc tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc 6541 ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa 6601 gcctccggag cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc 6661 ccagggggat ctgtgagttt ggggaccctt gattgttctt tctttttcgc tattgtaaaa 6721 ttcatgttat atggaggggg caaagttttc agggtgttgt ttagaatggg aagatgtccc 6781 ttgtatcacc atggaccctc atgataattt tgtttctttc actttctact ctgttgacaa 6841 ccattgtctc ctcttatttt cttttcattt tctgtaactt tttcgttaaa ctttagcttg 6901 catttgtaac gaatttttaa attcactttt gtttatttgt cagattgtaa gtactttctc 6961 taatcacttt tttttcaagg caatcagggt atattatatt gtacttcagc acagttttag 7021 agaacaattg ttataattaa atgataaggt agaatatttc tgcatataaa ttctggctgg 7081 cgtggaaata ttcttattgg tagaaacaac tacatcctgg tcatcatcct gcctttctct 7141 ttatggttac aatgatatac actgtttgag atgaggataa aatactctga gtccaaaccg 7201 ggcccctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg 7261 ctggttattg tgctgtctca tcattttggc aaagaattgt aatacgactc actatagggc 7321 gaattgatat gtctagatta gataaaagta aagtgattaa cagcgcatta gagctgctta 7381 atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc 7441 agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc ttagccattg 7501 agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc tggcaagatt 7561 ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc gatggagcaa 7621 aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa aatcaattag 7681 cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc agcgctgtgg 7741 ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct aaagaagaaa 7801 gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc gaattatttg 7861 atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata tgcggattag 7921 aaaaacaact taaatgtgaa agtgggtccg cgtacagcgg ctcccgggag ttctagggat 7981 ctgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg 8041 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc 8101 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa 8161 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag 8221 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg 8281 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg 8341 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa 8401 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg 8461 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca 8521 cggggacgtg gttttccttt gaaaaacacg atgataagga tccaccggag cttaccatga 8581 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca 8641 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc 8701 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg 8761 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccgga //