LOCUS Exported 5320 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Localization: Endosomes, Excitation: 590, Emission: 649. ACCESSION . VERSION . KEYWORDS mPlum-Endo-14 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5320) TITLE Davidson Red fluorescent proteins REFERENCE 2 (bases 1 to 5320) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5320 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1..678 /codon_start=1 /product="far-red monomeric derivative of DsRed fluorescent protein (Wang et al., 2004)" /label=mPlum /note="mammalian codon-optimized" /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH STGA" primer_bind 402..421 /label=mCherry-F /note="mCherry, forward primer" primer_bind 596..619 /label=DsRed1-C /note="DsRed1, forward primer" CDS 724..753 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" polyA_signal 1496..1617 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1533..1552) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1587..1606 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1624..2079) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1761..1782) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 1973..1992 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2106..2210 /gene="bla" /label=AmpR promoter promoter 2212..2569 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2235..2255) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2420..2555 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2482..2501 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2604..3398 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2658..2677) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3268..3287 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3586..3605) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3630..3677 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4006..4594 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4495..4514 /label=pBR322ori-F /note="pBR322 origin, forward primer" enhancer 4769..5072 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 5073..5276 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 5227..5247 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" ORIGIN 1 atggtgagca agggcgagga ggtcatcaag gagttcatgc gcttcaagga gcacatggag 61 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag 121 ggcacccaga ccgccaggct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac 181 atcctgtccc ctcagatcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc 241 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc 301 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc 361 tacaaggtga aggtgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag 421 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc 481 gagatgaaga tgaggctgag gctgaaggac ggcggccact acgacgccga ggtcaagacc 541 acctacatgg ccaagaagcc cgtgcagctg cccggcgcct acaagaccga catcaagctg 601 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg cgccgagggc 661 cgccactcca ccggcgcctc cggatcagat ctcgagctca agcttgatat cgaattcccc 721 atggagcaga agctgatctc cgaggaggac ctgaaccgga agaaactggt ggttgttggt 781 gatggagcct gtggaaagac atgcttgctc atagtcttca gcaaggacga gttcccagag 841 gtgtatgtgc ccacagtgtt tgagaactat gtggcagata tcgaggtgga tggaaagcag 901 gtagagttgg ctttgtggga cacagctggc caggaggact acgaccgcct gcggccgctc 961 tcctacccgg acaccgacgt cattctcatg tgcttctcgg tggacagccc ggactcgctg 1021 gagaacatcc ccgagaagtg ggtccccgag gtgaagcact tctgtcccaa tgtgcccatc 1081 atcctggtgg ccaacaaaaa agacctgcgc agcgacgagc atgtccgcac agagctggcc 1141 cgcatgaagc aggaacccgt tcgcacggat gacggccgcg ccatggccgt gcgcatccaa 1201 gcctacgact acctcgagtg ctctgccaag accaaggaag gcgtgcgcga ggtcttcgag 1261 acggccacgc gcgccgcgct gcagaagcgc tacggctccc agaacggctg catcaactgc 1321 tgcaaggtgc tatgagggcc gcgcccgtcg cgcctgcccc tgccggggat ccaccggatc 1381 tagataactg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa 1441 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt 1501 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 1561 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg 1621 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct 1681 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg 1741 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact 1801 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac 1861 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga 1921 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga 1981 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca 2041 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa 2101 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 2161 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg 2221 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc 2281 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 2341 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt 2401 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 2461 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 2521 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag 2581 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg 2641 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 2701 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 2761 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga 2821 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 2881 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 2941 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 3001 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 3061 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 3121 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct 3181 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 3241 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg 3301 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc 3361 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat 3421 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta 3481 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg 3541 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga 3601 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg 3661 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac 3721 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc 3781 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat 3841 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag 3901 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 3961 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 4021 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 4081 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 4141 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 4201 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 4261 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 4321 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 4381 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 4441 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 4501 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 4561 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 4621 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc 4681 tgtggataac cgtattaccg ccatgcatta gttattaata gtaatcaatt acggggtcat 4741 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg 4801 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa 4861 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact 4921 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta 4981 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt 5041 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg 5101 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg 5161 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc 5221 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt 5281 tagtgaaccg tcagatccgc tagcgctacc ggtggctacc //