LOCUS       Exported                 995 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  Fluorophore-friendly streptavidin that can improve fluorescent 
            brightness and has unimpaired biotin binding after dye labeling.
ACCESSION   .
VERSION     .
KEYWORDS    pET21-Flavidin
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Jacobsen MT, Fairhead M, Fogelstrand P, Howarth M
  TITLE     Amine Landscaping to Maximize Protein-Dye Fluorescence and 
            Ultrastable Protein-Ligand Interaction.
  JOURNAL   Cell Chem Biol. 2017 Jul 17. pii: S2451-9456(17)30229-5. doi: 
            10.1016/j.chembiol.2017.06.015.
  PUBMED    28757182
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             477..494
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(547..565)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      561..608
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttcccctcta nantaatttt gtttaacttt aagaaggaga tatacatatg gctgaagctg
       61 gtatcaccgg cacctggtac aaccagctgg gatccacctt catcgttacc gctggtgctg
      121 acggtgctct gaccggtacc tacgaatccg ctgttggtaa cgctgaatct agatacgttc
      181 tgaccggtcg ttacgactcc gctccggcta ccgacggttc cggaaccgct ctgggttgga
      241 ccgttgcttg gcgcaacaac taccgtaacg ctcactccgc taccacctgg tctggccagt
      301 acgttggtgg tgctgaagct cgtatcaaca cccagtggtt gttgacctcc ggcaccaccg
      361 aagctaacgc gtggcgatcc accctggttg gtcacgacac cttcacccgt gttcgtccgt
      421 ccgctgcttc cgaagaggaa gaggaagagt aataaaagct tgcggccgca ctcgagcacc
      481 accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg
      541 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
      601 gtttnttgct gaaaggagga actatatccg gattggcgaa ngggacgcgc cctgtagcgg
      661 cgcattaagc gcggcgtgtg tggnggttac gctcaacgtg atngcttcna ttgccatcac
      721 cctannnccc nctttttcgc nttcttccct tcctttctcg cctttttntn nnnttttncg
      781 ncannttcta atcttnngtt anntnnttnt tattnntgct naattnaccn cgnctccaan
      841 attttntttt gnnattggtt natnttttgt nnnntnnttn nntnnntntt tntatccttt
      901 tnnnnnttnt ttnnttnttn nnaatatnnn ntntnncnnn tgtnnnnnnn nctttctnnn
      961 nagtttnnnt nnntnnntnt nnacatnnnn nnntt
//
