LOCUS       Exported                8962 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  research of alternative isoforms of the protein dysferlin.
ACCESSION   .
VERSION     .
KEYWORDS    pUC57 DYSF, alt exon 1, 5a, 17
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8962)
  TITLE     Jain Foundation plasmids
REFERENCE   2  (bases 1 to 8962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8962
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     52..74
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     66..83
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     67..83
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             875..886
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6474..6490)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6474..6490)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6487..6509)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6498..6514
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6522..6552)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6567..6588
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6705..6722)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6876..7464)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6956..6975)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7635..8450)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8358..8377
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(8451..8555)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8623..8641
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8679..8701)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8801..8820
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
       61 gacgttgtaa aacgacggcc agtgaattga cgcgtattgg gatatgctga gggtcttcat
      121 cctctatgcc gagaacgtcc acacacccga caccgacatc agcgatgcct actgctccgc
      181 ggtgtttgca ggggtgaaga agagaaccaa agtcatcaag aacagcgtga accctgtatg
      241 gaatgaggga tttgaatggg acctcaaggg catccccctg gaccagggct ctgagcttca
      301 tgtggtggtc aaagaccatg agacgatggg gaggaacagg ttcctggggg aagccaaggt
      361 cccactccga gaggtcctcg ccacccctag tctgtccgcc agcttcaatg cccccctgct
      421 ggacaccaag aagcagccca caggggcctc gctggtcctg caggtgtcct acacaccgct
      481 gcctggagct gtgcccctgt tcccgccccc tactcctctg gagccctccc cgactctgcc
      541 tgacctggat gtagtggcag gcgggggaca gagccgggcc gagacttggt ccctgctcag
      601 tgacagcacc atggacacga gatactctgg aaagaagtgg ccggccccca cggacacagg
      661 aggagaggaa gacacagagg accagggact cactggagat gaggcggagc cattcctgga
      721 tcaaagcgga ggcccggggg ctcccaccac cccaaggaaa ctaccttcac gtcctccgcc
      781 ccactacccc gggatcaaaa gaaagcgaag tgcgcctaca tctagaaagc tgctgtcaga
      841 caaaccgcag gatttccaga tcagggtcca ggtgatcgag gggcgccagc tgccgggggt
      901 gaacatcaag cctgtggtca aggttaccgc tgcagggcag accaagcgga cgcggatcca
      961 caagggaaac agcccactct tcaatgagac tcttttcttc aacttgtttg actctcctgg
     1021 ggagctgttt gatgagccca tctttatcac ggtggtagac tctcgttctc tcaggacaga
     1081 tgctctcctc ggggagttcc ggatggacgt gggcaccatt tacagagagc cccggcacgc
     1141 ctatctcagg aagtggctgc tgctctcaga ccctgatgac ttctctgctg gggccagagg
     1201 ctacctgaaa acaagccttt gtgtgctggg gcctggggac gaagcgcctc tggagagaaa
     1261 agacccctct gaagacaagg aggacattga aagcaacctg ctccggccca caggcgtagc
     1321 cctgcgagga gcccacttct gcctgaaggt cttccgggcc gaggacttgc cgcagatgga
     1381 cgatgccgtg atggacaacg tgaaacagat ctttggcttc gagagtaaca agaagaactt
     1441 ggtggacccc tttgtggagg tcagctttgc ggggaaaatg ctgtgcagca agatcttgga
     1501 gaagacggcc aaccctcagt ggaaccagaa catcacactg cctgccatgt ttccctccat
     1561 gtgcgaaaaa atgaggattc gtatcataga ctgggaccgc ctgactcaca atgacatcgt
     1621 ggctaccacc tacctgagta tgtcgaaaat ctctgcccct ggaggagaaa tagaagagga
     1681 gcctgcaggt gctgtcaagc cttcgaaagc ctcagacttg gatgactacc tgggcttcct
     1741 ccccactttt gggccctgct acatcaacct ctatggcagt cccagagagt tcacaggctt
     1801 cccagacccc tacacagagc tcaacacagg caagggggaa ggtgtggctt atcgtggccg
     1861 gcttctgctc tccctggaga ccaagctggt ggagcacagt gaacagaagg tggaggacct
     1921 tcctgcggat gacatcctcc gggtggagaa gtaccttagg aggcgcaagt actccctgtt
     1981 tgcggccttc tactcagcca ccatgctgca ggatgtggat gacgccatcc agtttgaggt
     2041 cagcatcggg aactacggga acaagttcga catgacctgc ctgccgctgg cctccaccac
     2101 tcagtacagc cgtgcagtct ttgacgggtg ccactactac tacctaccct ggggtaacgt
     2161 gaaacccgtg gtggtgctgt catcctactg ggaggacatc agccatagaa tcgagactca
     2221 gaaccagctg cttgggattg ctgaccggct ggaagctggc ctggagcagg tccacctggc
     2281 cctgaaggcg cagtgctcca cggaggacgt ggactcgctg gtggctcagc tgacggatga
     2341 gctcatcgca ggctgcagcc agcctctggg tgacatccat gagacaccct ctgccaccca
     2401 cctggaccag tacctgtacc agctgcgcac ccatcacctg agccaaatca ctgaggctgc
     2461 cctggccctg aagctcggcc acagtgagct ccctgcagct ctggagcagg cggaggactg
     2521 gctcctgcgt ctgcgtgccc tggcagagga gccccagaac agcctgccgg acatcgtcat
     2581 ctggatgctg cagggagaca agcgtgtggc ataccagcgg gtgcctgccc accaagtcct
     2641 cttctcccgg cggggtgcca actactgtgg caagaattgt gggaagctac agacaatctt
     2701 tctgaaatat ccgatggaga aggtgcctgg cgcccggatg ccagtgcaga tacgggtcaa
     2761 gctgtggttt gggctctctg tggatgagaa ggagttcaac cagtttgctg aggggaagct
     2821 gtctgtcttt gctgaaacct atgagaacga gactaagttg gcccttgttg ggaactgggg
     2881 cacaacgggc ctcacctacc ccaagttttc tgacgtcacg ggcaagatca agctacccaa
     2941 ggacagcttc cgcccctcgg ccggctggac ctgggctgga gattggttcg tgtgcccgga
     3001 gaagactctg ctccatgaca tggacgccgg tcacctgagc ttcgtggaag aggtgtttga
     3061 gaaccagacc cggcttcccg gaggccagtg gatctacatg agtgacaact acaccgatgt
     3121 gaacggggag aaggtgcttc ccaaggatga cattgagtgc ccactgggct ggaagtggga
     3181 agatgaggaa tggtccacag acctcaaccg ggctgtcgat gagcaaggct gggagtatag
     3241 catcaccatc cccccggagc ggaagccgaa gcactgggtc cctgctgaga agatgtacta
     3301 cacacaccga cggcggcgct gggtgcgcct gcgcaggagg gatctcagcc aaatggaagc
     3361 actgaaaagg cacaggcagg cggaggcgga gggcgagggc tgggagtacg cctctctttt
     3421 tggctggaag ttccacctcg agtaccgcaa gacagatgcc ttccgccgcc gccgctggcg
     3481 ccgtcgcatg gagccactgg agaagacggg gcctgcagct gtgtttgccc ttgagggggc
     3541 cctgggcggc gtgatggatg acaagagtga agattccatg tccgtctcca ccttgagctt
     3601 cggtgtgaac agacccacga tttcctgcat attcgactat gggaaccgct accatctacg
     3661 ctgctacatg taccaggccc gggacctggc tgcgatggac aaggactctt tttctgatcc
     3721 ctatgccatc gtctccttcc tgcaccagag ccagaagacg gtggtggtga agaacaccct
     3781 taaccccacc tgggaccaga cgctcatctt ctacgagatc gagatctttg gcgagccggc
     3841 cacagttgct gagcaaccgc ccagcattgt ggtggagctg tacgaccatg acacttatgg
     3901 tgcagacgag tttatgggtc gctgcatctg tcaaccgagt ctggaacgga tgccacggct
     3961 ggcctggttc ccactgacga ggggcagcca gccgtcgggg gagctgctgg cctcttttga
     4021 gctcatccag agagagaagc cggccatcca ccatattcct ggttttgagg tgcaggagac
     4081 atcaaggatc ctggatgagt ctgaggacac agacctgccc tacccaccac cccagaggga
     4141 ggccaacatc tacatggttc ctcagaacat caagccagcg ctccagcgta ccgccatcga
     4201 gatcctggca tggggcctgc ggaacatgaa gagttaccag ctggccaaca tctcctcccc
     4261 cagcctcgtg gtagagtgtg ggggccagac ggtgcagtcc tgtgtcatca ggaacctccg
     4321 gaagaacccc aactttgaca tctgcaccct cttcatggaa gtgatgctgc ccagggagga
     4381 gctctactgc ccccccatca ccgtcaaggt catcgataac cgccagtttg gccgccggcc
     4441 tgtggtgggc cagtgtacca tccgctccct ggagagcttc ctgtgtgacc cctactcggc
     4501 ggagagtcca tccccacagg gtggcccaga cgatgtgagc ctactcagtc ctggggaaga
     4561 cgtgctcatc gacattgatg acaaggagcc cctcatcccc atccaggagg aagagttcat
     4621 cgattggtgg agcaaattct ttgcctccat aggggagagg gaaaagtgcg gctcctacct
     4681 ggagaaggat tttgacaccc tgaaggtcta tgacacacag ctggagaatg tggaggcctt
     4741 tgagggcctg tctgactttt gtaacacctt caagctgtac cggggcaaga cgcaggagga
     4801 gacagaagat ccatctgtga ttggtgaatt taagggcctc ttcaaaattt atcccctccc
     4861 agaagaccca gccatcccca tgcccccaag acagttccac cagctggccg cccagggacc
     4921 ccaggagtgc ttggtccgta tctacattgt ccgagcattt ggcctgcagc ccaaggaccc
     4981 caatggaaag tgtgatcctt acatcaagat ctccataggg aagaaatcag tgagtgacca
     5041 ggataactac atcccctgca cgctggagcc cgtatttgga aagatgttcg agctgacctg
     5101 cactctgcct ctggagaagg acctaaagat cactctctat gactatgacc tcctctccaa
     5161 ggacgaaaag atcggtgaga cggtcgtcga cctggagaac aggctgctgt ccaagtttgg
     5221 ggctcgctgt ggactcccac agacctactg tgtctctgga ccgaaccagt ggcgggacca
     5281 gctccgcccc tcccagctcc tccacctctt ctgccagcag catagagtca aggcacctgt
     5341 gtaccggaca gaccgtgtaa tgtttcagga taaagaatat tccattgaag agatagaggc
     5401 tggcaggatc ccaaacccac acctgggccc agtggaggag cgtctggctc tgcatgtgct
     5461 tcagcagcag ggcctggtcc cggagcacgt ggagtcacgg cccctctaca gccccctgca
     5521 gccagacatc gagcagggga agctgcagat gtgggtcgac ctatttccga aggccctggg
     5581 gcggcctgga cctcccttca acatcacccc acggagagcc agaaggtttt tcctgcgttg
     5641 tattatctgg aataccagag atgtgatcct ggatgacctg agcctcacgg gggagaagat
     5701 gagcgacatt tatgtgaaag gttggatgat tggctttgaa gaacacaagc aaaagacaga
     5761 cgtgcattat cgttccctgg gaggtgaagg caacttcaac tggaggttca ttttcccctt
     5821 cgactacctg ccagctgagc aagtctgtac cattgccaag aaggatgcct tctggaggct
     5881 ggacaagact gagagcaaaa tcccagcacg agtggtgttc cagatctggg acaatgacaa
     5941 gttctccttt gatgattttc tgggctccct gcagctcgat ctcaaccgca tgcccaagcc
     6001 agccaagaca gccaagaagt gctccttgga ccagctggat gatgctttcc acccagaatg
     6061 gtttgtgtcc ctttttgagc agaaaacagt gaagggctgg tggccctgtg tagcagaaga
     6121 gggtgagaag aaaatactgg cgggcaagct ggaaatgacc ttggagattg tagcagagag
     6181 tgagcatgag gagcggcctg ctggccaggg ccgggatgag cccaacatga accctaagct
     6241 tgaggaccca aggcgccccg acacctcctt cctgtggttt acctccccat acaagaccat
     6301 gaagttcatc ctgtggcggc gtttccggtg ggccatcatc ctcttcatca tcctcttcat
     6361 cctgctgctg ttcctggcca tcttcatcta cgccttcccg aactatgctg ccatgaagct
     6421 ggtgaagccc ttcagctgaa tcccaatggc gcgccgagct tggctcgagc atggtcatag
     6481 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     6541 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     6601 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     6661 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6721 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6781 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6841 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6901 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6961 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7021 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7081 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7141 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7201 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7261 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7321 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7381 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7441 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7501 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7561 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7621 acttggtctg acagttagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata
     7681 tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca
     7741 ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca
     7801 acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca
     7861 ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact
     7921 tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta
     7981 ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta
     8041 caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca
     8101 cctgaatcag gatattcttc taatacctgg aatgctgttt tcccagggat cgcagtggtg
     8161 agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat
     8221 tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg
     8281 ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca
     8341 cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg
     8401 gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa tatggctcat actcttcctt
     8461 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8521 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8581 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     8641 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     8701 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     8761 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     8821 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     8881 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     8941 tcttcgctat tacgccagct gg
//
