LOCUS Exported 8924 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION research of alternative isoforms of the protein dysferlin. ACCESSION . VERSION . KEYWORDS pUC57 DYSF, alt exon 1, 17, 40a SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8924) TITLE Jain Foundation plasmids REFERENCE 2 (bases 1 to 8924) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8924 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 24..46 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 38..55 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 39..55 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" CDS 746..757 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(6408..6424) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6408..6424) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6421..6443) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6432..6448 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6456..6486) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6501..6522 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(6639..6656) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6810..7398) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6890..6909) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7569..8384) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 8292..8311 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" promoter complement(8385..8489) /gene="bla" /label=AmpR promoter primer_bind 8557..8575 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(8613..8635) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 8735..8754 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt 61 gacgcgtatg ctgagggtct tcatcctcta tgccgagaac gtccacacac ccgacaccga 121 catcagcgat gcctactgct ccgcggtgtt tgcaggggtg aagaagagaa ccaaagtcat 181 caagaacagc gtgaaccctg tatggaatga gggatttgaa tgggacctca agggcatccc 241 cctggaccag ggctctgagc ttcatgtggt ggtcaaagac catgagacga tggggaggaa 301 caggttcctg ggggaagcca aggtcccact ccgagaggtc ctcgccaccc ctagtctgtc 361 cgccagcttc aatgcccccc tgctggacac caagaagcag cccacagggg cctcgctggt 421 cctgcaggtg tcctacacac cgctgcctgg agctgtgccc ctgttcccgc cccctactcc 481 tctggagccc tccccgactc tgcctgacct ggatgtagtg gcagacacag gaggagagga 541 agacacagag gaccagggac tcactggaga tgaggcggag ccattcctgg atcaaagcgg 601 aggcccgggg gctcccacca ccccaaggaa actaccttca cgtcctccgc cccactaccc 661 cgggatcaaa agaaagcgaa gtgcgcctac atctagaaag ctgctgtcag acaaaccgca 721 ggatttccag atcagggtcc aggtgatcga ggggcgccag ctgccggggg tgaacatcaa 781 gcctgtggtc aaggttaccg ctgcagggca gaccaagcgg acgcggatcc acaagggaaa 841 cagcccactc ttcaatgaga ctcttttctt caacttgttt gactctcctg gggagctgtt 901 tgatgagccc atctttatca cggtggtaga ctctcgttct ctcaggacag atgctctcct 961 cggggagttc cggatggacg tgggcaccat ttacagagag ccccggcacg cctatctcag 1021 gaagtggctg ctgctctcag accctgatga cttctctgct ggggccagag gctacctgaa 1081 aacaagcctt tgtgtgctgg ggcctgggga cgaagcgcct ctggagagaa aagacccctc 1141 tgaagacaag gaggacattg aaagcaacct gctccggccc acaggcgtag ccctgcgagg 1201 agcccacttc tgcctgaagg tcttccgggc cgaggacttg ccgcagatgg acgatgccgt 1261 gatggacaac gtgaaacaga tctttggctt cgagagtaac aagaagaact tggtggaccc 1321 ctttgtggag gtcagctttg cggggaaaat gctgtgcagc aagatcttgg agaagacggc 1381 caaccctcag tggaaccaga acatcacact gcctgccatg tttccctcca tgtgcgaaaa 1441 aatgaggatt cgtatcatag actgggaccg cctgactcac aatgacatcg tggctaccac 1501 ctacctgagt atgtcgaaaa tctctgcccc tggaggagaa atagaagagg agcctgcagg 1561 tgctgtcaag ccttcgaaag cctcagactt ggatgactac ctgggcttcc tccccacttt 1621 tgggccctgc tacatcaacc tctatggcag tcccagagag ttcacaggct tcccagaccc 1681 ctacacagag ctcaacacag gcaaggggga aggtgtggct tatcgtggcc ggcttctgct 1741 ctccctggag accaagctgg tggagcacag tgaacagaag gtggaggacc ttcctgcgga 1801 tgacatcctc cgggtggaga agtaccttag gaggcgcaag tactccctgt ttgcggcctt 1861 ctactcagcc accatgctgc aggatgtgga tgacgccatc cagtttgagg tcagcatcgg 1921 gaactacggg aacaagttcg acatgacctg cctgccgctg gcctccacca ctcagtacag 1981 ccgtgcagtc tttgacgggt gccactacta ctacctaccc tggggtaacg tgaaacccgt 2041 ggtggtgctg tcatcctact gggaggacat cagccataga atcgagactc agaaccagct 2101 gcttgggatt gctgaccggc tggaagctgg cctggagcag gtccacctgg ccctgaaggc 2161 gcagtgctcc acggaggacg tggactcgct ggtggctcag ctgacggatg agctcatcgc 2221 aggctgcagc cagcctctgg gtgacatcca tgagacaccc tctgccaccc acctggacca 2281 gtacctgtac cagctgcgca cccatcacct gagccaaatc actgaggctg ccctggccct 2341 gaagctcggc cacagtgagc tccctgcagc tctggagcag gcggaggact ggctcctgcg 2401 tctgcgtgcc ctggcagagg agccccagaa cagcctgccg gacatcgtca tctggatgct 2461 gcagggagac aagcgtgtgg cataccagcg ggtgcctgcc caccaagtcc tcttctcccg 2521 gcggggtgcc aactactgtg gcaagaattg tgggaagcta cagacaatct ttctgaaata 2581 tccgatggag aaggtgcctg gcgcccggat gccagtgcag atacgggtca agctgtggtt 2641 tgggctctct gtggatgaga aggagttcaa ccagtttgct gaggggaagc tgtctgtctt 2701 tgctgaaacc tatgagaacg agactaagtt ggcccttgtt gggaactggg gcacaacggg 2761 cctcacctac cccaagtttt ctgacgtcac gggcaagatc aagctaccca aggacagctt 2821 ccgcccctcg gccggctgga cctgggctgg agattggttc gtgtgcccgg agaagactct 2881 gctccatgac atggacgccg gtcacctgag cttcgtggaa gaggtgtttg agaaccagac 2941 ccggcttccc ggaggccagt ggatctacat gagtgacaac tacaccgatg tgaacgggga 3001 gaaggtgctt cccaaggatg acattgagtg cccactgggc tggaagtggg aagatgagga 3061 atggtccaca gacctcaacc gggctgtcga tgagcaaggc tgggagtata gcatcaccat 3121 ccccccggag cggaagccga agcactgggt ccctgctgag aagatgtact acacacaccg 3181 acggcggcgc tgggtgcgcc tgcgcaggag ggatctcagc caaatggaag cactgaaaag 3241 gcacaggcag gcggaggcgg agggcgaggg ctgggagtac gcctctcttt ttggctggaa 3301 gttccacctc gagtaccgca agacagatgc cttccgccgc cgccgctggc gccgtcgcat 3361 ggagccactg gagaagacgg ggcctgcagc tgtgtttgcc cttgaggggg ccctgggcgg 3421 cgtgatggat gacaagagtg aagattccat gtccgtctcc accttgagct tcggtgtgaa 3481 cagacccacg atttcctgca tattcgacta tgggaaccgc taccatctac gctgctacat 3541 gtaccaggcc cgggacctgg ctgcgatgga caaggactct ttttctgatc cctatgccat 3601 cgtctccttc ctgcaccaga gccagaagac ggtggtggtg aagaacaccc ttaaccccac 3661 ctgggaccag acgctcatct tctacgagat cgagatcttt ggcgagccgg ccacagttgc 3721 tgagcaaccg cccagcattg tggtggagct gtacgaccat gacacttatg gtgcagacga 3781 gtttatgggt cgctgcatct gtcaaccgag tctggaacgg atgccacggc tggcctggtt 3841 cccactgacg aggggcagcc agccgtcggg ggagctgctg gcctcttttg agctcatcca 3901 gagagagaag ccggccatcc accatattcc tggttttgag gtgcaggaga catcaaggat 3961 cctggatgag tctgaggaca cagacctgcc ctacccacca ccccagaggg aggccaacat 4021 ctacatggtt cctcagaaca tcaagccagc gctccagcgt accgccatcg agatcctggc 4081 atggggcctg cggaacatga agagttacca gctggccaac atctcctccc ccagcctcgt 4141 ggtagagtgt gggggccaga cggtgcagtc ctgtgtcatc aggaacctcc ggaagaaccc 4201 caactttgac atctgcaccc tcttcatgga agtgatgctg cccagggagg agctctactg 4261 cccccccatc accgtcaagg tcatcgataa ccgccagttt ggccgccggc ctgtggtggg 4321 ccagtgtacc atccgctccc tggagagctt cctgtgtgac ccctactcgg cggagagtcc 4381 atccccacag ggtggcccag acgatgtgag cctactcagt cctggggaag acgtgctcat 4441 cgacattgat gacaaggagc ccctcatccc catccagctt gcagacggtc tgtcgagctt 4501 ggcccccact aacacggctt ctcctccatc cagtcctcat gaggaagagt tcatcgattg 4561 gtggagcaaa ttctttgcct ccatagggga gagggaaaag tgcggctcct acctggagaa 4621 ggattttgac accctgaagg tctatgacac acagctggag aatgtggagg cctttgaggg 4681 cctgtctgac ttttgtaaca ccttcaagct gtaccggggc aagacgcagg aggagacaga 4741 agatccatct gtgattggtg aatttaaggg cctcttcaaa atttatcccc tcccagaaga 4801 cccagccatc cccatgcccc caagacagtt ccaccagctg gccgcccagg gaccccagga 4861 gtgcttggtc cgtatctaca ttgtccgagc atttggcctg cagcccaagg accccaatgg 4921 aaagtgtgat ccttacatca agatctccat agggaagaaa tcagtgagtg accaggataa 4981 ctacatcccc tgcacgctgg agcccgtatt tggaaagatg ttcgagctga cctgcactct 5041 gcctctggag aaggacctaa agatcactct ctatgactat gacctcctct ccaaggacga 5101 aaagatcggt gagacggtcg tcgacctgga gaacaggctg ctgtccaagt ttggggctcg 5161 ctgtggactc ccacagacct actgtgtctc tggaccgaac cagtggcggg accagctccg 5221 cccctcccag ctcctccacc tcttctgcca gcagcataga gtcaaggcac ctgtgtaccg 5281 gacagaccgt gtaatgtttc aggataaaga atattccatt gaagagatag aggctggcag 5341 gatcccaaac ccacacctgg gcccagtgga ggagcgtctg gctctgcatg tgcttcagca 5401 gcagggcctg gtcccggagc acgtggagtc acggcccctc tacagccccc tgcagccaga 5461 catcgagcag gggaagctgc agatgtgggt cgacctattt ccgaaggccc tggggcggcc 5521 tggacctccc ttcaacatca ccccacggag agccagaagg tttttcctgc gttgtattat 5581 ctggaatacc agagatgtga tcctggatga cctgagcctc acgggggaga agatgagcga 5641 catttatgtg aaaggttgga tgattggctt tgaagaacac aagcaaaaga cagacgtgca 5701 ttatcgttcc ctgggaggtg aaggcaactt caactggagg ttcattttcc ccttcgacta 5761 cctgccagct gagcaagtct gtaccattgc caagaaggat gccttctgga ggctggacaa 5821 gactgagagc aaaatcccag cacgagtggt gttccagatc tgggacaatg acaagttctc 5881 ctttgatgat tttctgggct ccctgcagct cgatctcaac cgcatgccca agccagccaa 5941 gacagccaag aagtgctcct tggaccagct ggatgatgct ttccacccag aatggtttgt 6001 gtcccttttt gagcagaaaa cagtgaaggg ctggtggccc tgtgtagcag aagagggtga 6061 gaagaaaata ctggcgggca agctggaaat gaccttggag attgtagcag agagtgagca 6121 tgaggagcgg cctgctggcc agggccggga tgagcccaac atgaacccta agcttgagga 6181 cccaaggcgc cccgacacct ccttcctgtg gtttacctcc ccatacaaga ccatgaagtt 6241 catcctgtgg cggcgtttcc ggtgggccat catcctcttc atcatcctct tcatcctgct 6301 gctgttcctg gccatcttca tctacgcctt cccgaactat gctgccatga agctggtgaa 6361 gcccttcagc tgaatcccaa tggcgcgccg agcttggctc gagcatggtc atagctgttt 6421 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 6481 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 6541 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 6601 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 6661 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 6721 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 6781 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 6841 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 6901 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 6961 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 7021 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 7081 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 7141 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 7201 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 7261 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 7321 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 7381 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 7441 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 7501 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 7561 tctgacagtt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga 7621 ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg 7681 cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca 7741 atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga 7801 gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca 7861 acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt 7921 cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca 7981 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa 8041 tcaggatatt cttctaatac ctggaatgct gttttcccag ggatcgcagt ggtgagtaac 8101 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc 8161 agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt 8221 ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat 8281 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt 8341 aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcatactctt cctttttcaa 8401 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 8461 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 8521 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt 8581 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg 8641 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg 8701 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga 8761 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg 8821 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg 8881 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gatt //