LOCUS       Exported                8927 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  research of alternative isoforms of the protein dysferlin.
ACCESSION   .
VERSION     .
KEYWORDS    pUC57 DYSF, alt exon 1a, 17, 40a
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8927)
  TITLE     Jain Foundation plasmids
REFERENCE   2  (bases 1 to 8927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8927
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     20..42
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     34..51
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     35..51
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             745..756
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6407..6423)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6407..6423)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6420..6442)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6431..6447
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6455..6485)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6500..6521
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6638..6655)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6809..7397)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6889..6908)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7568..8383)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8291..8310
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(8384..8488)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8556..8574
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8612..8634)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8734..8753
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgacg
       61 cgtatgctgt gctgcctgct ggtgagggcc agcaacctcc ccagtgcgaa gaaggaccgg
      121 cgcagcgacc ctgtcgcaag cctgactttc cgaggggtga agaagagaac caaagtcatc
      181 aagaacagcg tgaaccctgt atggaatgag ggatttgaat gggacctcaa gggcatcccc
      241 ctggaccagg gctctgagct tcatgtggtg gtcaaagacc atgagacgat ggggaggaac
      301 aggttcctgg gggaagccaa ggtcccactc cgagaggtcc tcgccacccc tagtctgtcc
      361 gccagcttca atgcccccct gctggacacc aagaagcagc ccacaggggc ctcgctggtc
      421 ctgcaggtgt cctacacacc gctgcctgga gctgtgcccc tgttcccgcc ccctactcct
      481 ctggagccct ccccgactct gcctgacctg gatgtagtgg cagacacagg aggagaggaa
      541 gacacagagg accagggact cactggagat gaggcggagc cattcctgga tcaaagcgga
      601 ggcccggggg ctcccaccac cccaaggaaa ctaccttcac gtcctccgcc ccactacccc
      661 gggatcaaaa gaaagcgaag tgcgcctaca tctagaaagc tgctgtcaga caaaccgcag
      721 gatttccaga tcagggtcca ggtgatcgag gggcgccagc tgccgggggt gaacatcaag
      781 cctgtggtca aggttaccgc tgcagggcag accaagcgga cgcggatcca caagggaaac
      841 agcccactct tcaatgagac tcttttcttc aacttgtttg actctcctgg ggagctgttt
      901 gatgagccca tctttatcac ggtggtagac tctcgttctc tcaggacaga tgctctcctc
      961 ggggagttcc ggatggacgt gggcaccatt tacagagagc cccggcacgc ctatctcagg
     1021 aagtggctgc tgctctcaga ccctgatgac ttctctgctg gggccagagg ctacctgaaa
     1081 acaagccttt gtgtgctggg gcctggggac gaagcgcctc tggagagaaa agacccctct
     1141 gaagacaagg aggacattga aagcaacctg ctccggccca caggcgtagc cctgcgagga
     1201 gcccacttct gcctgaaggt cttccgggcc gaggacttgc cgcagatgga cgatgccgtg
     1261 atggacaacg tgaaacagat ctttggcttc gagagtaaca agaagaactt ggtggacccc
     1321 tttgtggagg tcagctttgc ggggaaaatg ctgtgcagca agatcttgga gaagacggcc
     1381 aaccctcagt ggaaccagaa catcacactg cctgccatgt ttccctccat gtgcgaaaaa
     1441 atgaggattc gtatcataga ctgggaccgc ctgactcaca atgacatcgt ggctaccacc
     1501 tacctgagta tgtcgaaaat ctctgcccct ggaggagaaa tagaagagga gcctgcaggt
     1561 gctgtcaagc cttcgaaagc ctcagacttg gatgactacc tgggcttcct ccccactttt
     1621 gggccctgct acatcaacct ctatggcagt cccagagagt tcacaggctt cccagacccc
     1681 tacacagagc tcaacacagg caagggggaa ggtgtggctt atcgtggccg gcttctgctc
     1741 tccctggaga ccaagctggt ggagcacagt gaacagaagg tggaggacct tcctgcggat
     1801 gacatcctcc gggtggagaa gtaccttagg aggcgcaagt actccctgtt tgcggccttc
     1861 tactcagcca ccatgctgca ggatgtggat gacgccatcc agtttgaggt cagcatcggg
     1921 aactacggga acaagttcga catgacctgc ctgccgctgg cctccaccac tcagtacagc
     1981 cgtgcagtct ttgacgggtg ccactactac tacctaccct ggggtaacgt gaaacccgtg
     2041 gtggtgctgt catcctactg ggaggacatc agccatagaa tcgagactca gaaccagctg
     2101 cttgggattg ctgaccggct ggaagctggc ctggagcagg tccacctggc cctgaaggcg
     2161 cagtgctcca cggaggacgt ggactcgctg gtggctcagc tgacggatga gctcatcgca
     2221 ggctgcagcc agcctctggg tgacatccat gagacaccct ctgccaccca cctggaccag
     2281 tacctgtacc agctgcgcac ccatcacctg agccaaatca ctgaggctgc cctggccctg
     2341 aagctcggcc acagtgagct ccctgcagct ctggagcagg cggaggactg gctcctgcgt
     2401 ctgcgtgccc tggcagagga gccccagaac agcctgccgg acatcgtcat ctggatgctg
     2461 cagggagaca agcgtgtggc ataccagcgg gtgcctgccc accaagtcct cttctcccgg
     2521 cggggtgcca actactgtgg caagaattgt gggaagctac agacaatctt tctgaaatat
     2581 ccgatggaga aggtgcctgg cgcccggatg ccagtgcaga tacgggtcaa gctgtggttt
     2641 gggctctctg tggatgagaa ggagttcaac cagtttgctg aggggaagct gtctgtcttt
     2701 gctgaaacct atgagaacga gactaagttg gcccttgttg ggaactgggg cacaacgggc
     2761 ctcacctacc ccaagttttc tgacgtcacg ggcaagatca agctacccaa ggacagcttc
     2821 cgcccctcgg ccggctggac ctgggctgga gattggttcg tgtgcccgga gaagactctg
     2881 ctccatgaca tggacgccgg tcacctgagc ttcgtggaag aggtgtttga gaaccagacc
     2941 cggcttcccg gaggccagtg gatctacatg agtgacaact acaccgatgt gaacggggag
     3001 aaggtgcttc ccaaggatga cattgagtgc ccactgggct ggaagtggga agatgaggaa
     3061 tggtccacag acctcaaccg ggctgtcgat gagcaaggct gggagtatag catcaccatc
     3121 cccccggagc ggaagccgaa gcactgggtc cctgctgaga agatgtacta cacacaccga
     3181 cggcggcgct gggtgcgcct gcgcaggagg gatctcagcc aaatggaagc actgaaaagg
     3241 cacaggcagg cggaggcgga gggcgagggc tgggagtacg cctctctttt tggctggaag
     3301 ttccacctcg agtaccgcaa gacagatgcc ttccgccgcc gccgctggcg ccgtcgcatg
     3361 gagccactgg agaagacggg gcctgcagct gtgtttgccc ttgagggggc cctgggcggc
     3421 gtgatggatg acaagagtga agattccatg tccgtctcca ccttgagctt cggtgtgaac
     3481 agacccacga tttcctgcat attcgactat gggaaccgct accatctacg ctgctacatg
     3541 taccaggccc gggacctggc tgcgatggac aaggactctt tttctgatcc ctatgccatc
     3601 gtctccttcc tgcaccagag ccagaagacg gtggtggtga agaacaccct taaccccacc
     3661 tgggaccaga cgctcatctt ctacgagatc gagatctttg gcgagccggc cacagttgct
     3721 gagcaaccgc ccagcattgt ggtggagctg tacgaccatg acacttatgg tgcagacgag
     3781 tttatgggtc gctgcatctg tcaaccgagt ctggaacgga tgccacggct ggcctggttc
     3841 ccactgacga ggggcagcca gccgtcgggg gagctgctgg cctcttttga gctcatccag
     3901 agagagaagc cggccatcca ccatattcct ggttttgagg tgcaggagac atcaaggatc
     3961 ctggatgagt ctgaggacac agacctgccc tacccaccac cccagaggga ggccaacatc
     4021 tacatggttc ctcagaacat caagccagcg ctccagcgta ccgccatcga gatcctggca
     4081 tggggcctgc ggaacatgaa gagttaccag ctggccaaca tctcctcccc cagcctcgtg
     4141 gtagagtgtg ggggccagac ggtgcagtcc tgtgtcatca ggaacctccg gaagaacccc
     4201 aactttgaca tctgcaccct cttcatggaa gtgatgctgc ccagggagga gctctactgc
     4261 ccccccatca ccgtcaaggt catcgataac cgccagtttg gccgccggcc tgtggtgggc
     4321 cagtgtacca tccgctccct ggagagcttc ctgtgtgacc cctactcggc ggagagtcca
     4381 tccccacagg gtggcccaga cgatgtgagc ctactcagtc ctggggaaga cgtgctcatc
     4441 gacattgatg acaaggagcc cctcatcccc atccagcttg cagacggtct gtcgagcttg
     4501 gcccccacta acacggcttc tcctccatcc agtcctcatg aggaagagtt catcgattgg
     4561 tggagcaaat tctttgcctc cataggggag agggaaaagt gcggctccta cctggagaag
     4621 gattttgaca ccctgaaggt ctatgacaca cagctggaga atgtggaggc ctttgagggc
     4681 ctgtctgact tttgtaacac cttcaagctg taccggggca agacgcagga ggagacagaa
     4741 gatccatctg tgattggtga atttaagggc ctcttcaaaa tttatcccct cccagaagac
     4801 ccagccatcc ccatgccccc aagacagttc caccagctgg ccgcccaggg accccaggag
     4861 tgcttggtcc gtatctacat tgtccgagca tttggcctgc agcccaagga ccccaatgga
     4921 aagtgtgatc cttacatcaa gatctccata gggaagaaat cagtgagtga ccaggataac
     4981 tacatcccct gcacgctgga gcccgtattt ggaaagatgt tcgagctgac ctgcactctg
     5041 cctctggaga aggacctaaa gatcactctc tatgactatg acctcctctc caaggacgaa
     5101 aagatcggtg agacggtcgt cgacctggag aacaggctgc tgtccaagtt tggggctcgc
     5161 tgtggactcc cacagaccta ctgtgtctct ggaccgaacc agtggcggga ccagctccgc
     5221 ccctcccagc tcctccacct cttctgccag cagcatagag tcaaggcacc tgtgtaccgg
     5281 acagaccgtg taatgtttca ggataaagaa tattccattg aagagataga ggctggcagg
     5341 atcccaaacc cacacctggg cccagtggag gagcgtctgg ctctgcatgt gcttcagcag
     5401 cagggcctgg tcccggagca cgtggagtca cggcccctct acagccccct gcagccagac
     5461 atcgagcagg ggaagctgca gatgtgggtc gacctatttc cgaaggccct ggggcggcct
     5521 ggacctccct tcaacatcac cccacggaga gccagaaggt ttttcctgcg ttgtattatc
     5581 tggaatacca gagatgtgat cctggatgac ctgagcctca cgggggagaa gatgagcgac
     5641 atttatgtga aaggttggat gattggcttt gaagaacaca agcaaaagac agacgtgcat
     5701 tatcgttccc tgggaggtga aggcaacttc aactggaggt tcattttccc cttcgactac
     5761 ctgccagctg agcaagtctg taccattgcc aagaaggatg ccttctggag gctggacaag
     5821 actgagagca aaatcccagc acgagtggtg ttccagatct gggacaatga caagttctcc
     5881 tttgatgatt ttctgggctc cctgcagctc gatctcaacc gcatgcccaa gccagccaag
     5941 acagccaaga agtgctcctt ggaccagctg gatgatgctt tccacccaga atggtttgtg
     6001 tccctttttg agcagaaaac agtgaagggc tggtggccct gtgtagcaga agagggtgag
     6061 aagaaaatac tggcgggcaa gctggaaatg accttggaga ttgtagcaga gagtgagcat
     6121 gaggagcggc ctgctggcca gggccgggat gagcccaaca tgaaccctaa gcttgaggac
     6181 ccaaggcgcc ccgacacctc cttcctgtgg tttacctccc catacaagac catgaagttc
     6241 atcctgtggc ggcgtttccg gtgggccatc atcctcttca tcatcctctt catcctgctg
     6301 ctgttcctgg ccatcttcat ctacgccttc ccgaactatg ctgccatgaa gctggtgaag
     6361 cccttcagct gaatcccaat ggcgcgccga gcttggctcg agcatggtca tagctgtttc
     6421 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     6481 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     6541 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     6601 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6661 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6721 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6781 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6841 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6901 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6961 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7021 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7081 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7141 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7201 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7261 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7321 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7381 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7441 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7501 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7561 ctgacagtta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat
     7621 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
     7681 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa
     7741 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
     7801 tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa
     7861 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc
     7921 gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag
     7981 gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat
     8041 caggatattc ttctaatacc tggaatgctg ttttcccagg gatcgcagtg gtgagtaacc
     8101 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca
     8161 gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt
     8221 tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt
     8281 gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta
     8341 atcgcggcct agagcaagac gtttcccgtt gaatatggct catactcttc ctttttcaat
     8401 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8461 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     8521 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     8581 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     8641 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     8701 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     8761 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     8821 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     8881 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagt
//
