LOCUS       Exported                8545 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses V5 tagged human NIPP1.
ACCESSION   .
VERSION     .
KEYWORDS    pLX302-NIPP1-V5 puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8545)
  AUTHORS   Shah M, Smolko CM, Kinicki S, Chapman ZD, Brautigan DL, Janes KA
  TITLE     Profiling Subcellular Protein Phosphatase Responses to 
            Coxsackievirus B3 Infection of Cardiomyocytes.
  JOURNAL   Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S244-S262. doi: 
            10.1074/mcp.O116.063487. Epub 2017 Feb 7.
  PUBMED    28174228
REFERENCE   2  (bases 1 to 8545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8545
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      35..623
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     524..543
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     777..794
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    911..932
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        947..977
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    985..1001
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     990..1012
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1009..1025
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1009..1025
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1044..1064
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1046..1064
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1092..1318
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1319..1499
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1546..1671
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2164..2397
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2582..2626
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2801..3301
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3220..3238
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3323..3922
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3323..3342)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3819..3839
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3964..4081
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4198..4501
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4502..4705
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4656..4676
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4728..4752
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5380..5404)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5406..5447
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5489..6077
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5542..5562)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5960..5971)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6149..6382
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6460..6581
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6497..6516)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6551..6570
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6621..6756
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6683..6702
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(6776..6795)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6777..6795)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6805..6822)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6805..6821)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6814..6836)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6963..7418
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7050..7069)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7260..7281
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7444..7548
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7549..8409
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7767..7786)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
       61 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
      121 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
      181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
      241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      541 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
      601 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
      661 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
      721 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
      781 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
      841 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
      901 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
      961 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     1021 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     1081 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     1141 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
     1201 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     1261 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
     1321 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     1381 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     1441 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     1501 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     1561 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     1621 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     1681 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     1741 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     1801 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     1861 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     1921 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     1981 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     2041 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     2101 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     2161 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     2221 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     2281 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     2341 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     2401 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     2461 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2521 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2581 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2641 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2701 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2761 attatcgttt cagacccacc tcccaacccc gaggggaccc ttgcgccttt tccaaggcag
     2821 ccctgggttt gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc
     2881 cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc
     2941 tacccttgtg ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt
     3001 gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc
     3061 agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag
     3121 cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg
     3181 gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc
     3241 tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca
     3301 gggggtacca ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     3361 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     3421 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     3481 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt
     3541 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     3601 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     3661 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     3721 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     3781 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     3841 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     3901 gacccgcaag cccggtgcct gagaattcta gatcttgaga caaatggcag tattcatcca
     3961 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     4021 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     4081 tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg gggcccgggg
     4141 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     4201 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     4261 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     4321 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     4381 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     4441 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     4501 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     4561 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     4621 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     4681 gtgggaggtc tatataagca gagctctctg gctaactgtc gggatcaaca agtttgtaca
     4741 aaaaagttgg catgggtgga gaggatgatg aactcaaggg cttactgggg cttccagagg
     4801 aggaaactga gcttgataac ctgacagagt tcaacactgc ccacaacaag cggatttcta
     4861 cccttaccat tgaggaggga aatctggaca ttcaaagacc aaagaggaag aggaagaact
     4921 cacgggtgac attcagtgag gatgatgaga tcatcaaccc agaggatgtg gatccctcag
     4981 ttggtcgatt caggaacatg gtgcaaactg cagtggtccc agtcaagaag aagcgtgtgg
     5041 agggccctgg ctccctgggc ctggaggaat cagggagcag gcgcatgcag aactttgcct
     5101 tcagcggagg actctacggg ggcctgcccc ccacacacag tgaagcaggc tcccagccac
     5161 atggcatcca tgggacagca ctcatcggtg gcttgcccat gccataccca aaccttgccc
     5221 ctgatgtgga cttgactcct gttgtgccgt cagcagtgaa catgaaccct gcaccaaacc
     5281 ctgcagtcta taaccctgaa gctgtaaatg aacccaagaa gaagaaatat gcaaaagagg
     5341 cttggccagg caagaagccc acaccttcct tgctgatttg cccaactttc ttgtacaaag
     5401 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
     5461 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     5521 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     5581 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     5641 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     5701 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     5761 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     5821 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     5881 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     5941 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     6001 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     6061 ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt
     6121 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     6181 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     6241 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     6301 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     6361 tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat
     6421 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     6481 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     6541 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     6601 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     6661 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     6721 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     6781 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     6841 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     6901 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     6961 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     7021 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     7081 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     7141 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     7201 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     7261 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     7321 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     7381 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     7441 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     7501 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     7561 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     7621 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     7681 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     7741 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     7801 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     7861 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     7921 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     7981 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     8041 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     8101 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     8161 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     8221 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     8281 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     8341 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     8401 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     8461 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     8521 taacgtgagt tttcgttcca ctgag
//
