LOCUS Exported 2519 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION For library amplification of fragment NG-7. ACCESSION . VERSION . KEYWORDS NG-7 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2519) AUTHORS Gogolok S, Garcia-Diaz C, Pollard SM TITLE STAR: a simple TAL effector assembly reaction using isothermal assembly. JOURNAL Sci Rep. 2016 Sep 12;6:33209. doi: 10.1038/srep33209. PUBMED 27615025 REFERENCE 2 (bases 1 to 2519) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2519 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 278..300 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 292..309 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 293..309 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 320..339 /label=T7 /note="T7 promoter, forward primer" promoter 320..338 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(662..679) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(772..1360) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(852..871) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1531..2391) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2154..2173 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2392..2496) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt 181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca 361 aggccacgtg tcttgtccag agctcgtatc ctgcagttaa caccggatca agtcgtggca 421 atcgcgtcga atggaggggg aaagcaagcc ctggaaaccg tgcaaaggtt gttgccggtc 481 ctgtgtcagg cgcatggatt aacgcccgca caggttgttc tgcaggatac ggtacctgga 541 gcacaagact ggcctcatgg gccttccgct cactgcccgc tttccagtcg ggaaacctgt 601 cgtgccagct gcattaacat ggtcatagct gtttccttgc gtattgggcg ctctccgctt 661 cctcgctcac tgactcgctg cgctcggtcg ttcgggtaaa gcctggggtg cctaatgagc 721 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 781 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 841 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 901 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 961 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 1021 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 1081 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 1141 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 1201 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 1261 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 1321 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 1381 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 1441 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 1501 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 1561 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 1621 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagaaccacg 1681 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 1741 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 1801 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 1861 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 1921 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 1981 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 2041 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 2101 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 2161 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 2221 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 2281 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 2341 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 2401 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 2461 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccac //