LOCUS Exported 2741 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION For library amplification of fragment NI-12. ACCESSION . VERSION . KEYWORDS NI-12 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2741) AUTHORS Gogolok S, Garcia-Diaz C, Pollard SM TITLE STAR: a simple TAL effector assembly reaction using isothermal assembly. JOURNAL Sci Rep. 2016 Sep 12;6:33209. doi: 10.1038/srep33209. PUBMED 27615025 REFERENCE 2 (bases 1 to 2741) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2741 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 278..300 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 292..309 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 293..309 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 320..339 /label=T7 /note="T7 promoter, forward primer" promoter 320..338 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(884..901) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(994..1582) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1074..1093) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1753..2613) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2376..2395 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2614..2718) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt 181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca 361 aggccacgtg tcttgtccag agctcgtatc ctgcagttaa cgccggccca agtagtcgca 421 atcgcgtcga acattggggg aaagcaagcc ctggaaaccg tgcaaaggtt gttgccggta 481 ttatgtcaag atcatgggct tcagactcga acaagcgggc gctgctcgac gcactggccg 541 aagccatgct ggcggagtcg agaatcatag cacttcggtg ccgagagccg acgacgactg 601 gcgctcattt cgagtctgac tgggaatgcc cgcagcttca ggcaggcgct gctcgcctac 661 cgccagcaga gtccaatgga tctcgaggaa ttcactcctc aggtgcaggc tgcctatcag 721 gagtcccatt gtgctggcgc gctgcaggat acggtacctg gagcacaaga ctggcctcat 781 gggccttccg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaac 841 atggtcatag ctgtttcctt gcgtattggg cgctctccgc ttcctcgctc actgactcgc 901 tgcgctcggt cgttcgggta aagcctgggg tgcctaatga gcaaaaggcc agcaaaaggc 961 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 1021 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 1081 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 1141 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 1201 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 1261 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 1321 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 1381 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 1441 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 1501 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 1561 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 1621 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 1681 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 1741 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 1801 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 1861 accatctggc cccagtgctg caatgatacc gcgagaacca cgctcaccgg ctccagattt 1921 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 1981 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 2041 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 2101 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 2161 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 2221 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 2281 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 2341 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 2401 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 2461 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 2521 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 2581 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 2641 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 2701 acaaataggg gttccgcgca catttccccg aaaagtgcca c //