LOCUS Exported 2767 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION For library amplification of fragment NI-13. ACCESSION . VERSION . KEYWORDS NI-13 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2767) AUTHORS Gogolok S, Garcia-Diaz C, Pollard SM TITLE STAR: a simple TAL effector assembly reaction using isothermal assembly. JOURNAL Sci Rep. 2016 Sep 12;6:33209. doi: 10.1038/srep33209. PUBMED 27615025 REFERENCE 2 (bases 1 to 2767) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2767 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 278..300 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 292..309 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 293..309 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 320..339 /label=T7 /note="T7 promoter, forward primer" promoter 320..338 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(910..927) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1020..1608) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1100..1119) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1779..2639) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2402..2421 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2640..2744) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt 181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca 361 aggccacgtg tcttgtccag agctcgtatc ctgcagccat tgtgctggcg cggattcttt 421 atcactgata agttggtgga catattatga gtcgtttatc agtgataaag tgtcaagcat 481 gacaaagttg cagccgaata cagtggagtc atccgtgccg ccctagacct gttgaacgag 541 gtcggcgtag acggtctgac gagagtccac gcaaactggc ggaacggttg ggggttcagc 601 agccggcgct ttagagtcct ggcgtattat gtcaagatca tgggctcacg ccagatcagg 661 ttgttgcaat cgcgtcgaac attgggggaa agcaagccct ggaaaccgtg caaaggttgt 721 tgccggtctt gtgtcaggac catggcttaa cgccggagca agttgtactg caggatacgg 781 tacctggagc acaagactgg cctcatgggc cttccgctca ctgcccgctt tccagtcggg 841 aaacctgtcg tgccagctgc attaacatgg tcatagctgt ttccttgcgt attgggcgct 901 ctccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cgggtaaagc ctggggtgcc 961 taatgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 1021 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 1081 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 1141 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 1201 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 1261 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 1321 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 1381 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 1441 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 1501 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 1561 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 1621 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 1681 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 1741 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 1801 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 1861 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 1921 gaaccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 1981 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 2041 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 2101 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 2161 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 2221 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 2281 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 2341 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 2401 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 2461 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 2521 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 2581 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 2641 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 2701 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 2761 gtgccac //