LOCUS Exported 2737 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION For library amplification of fragment NH-12. ACCESSION . VERSION . KEYWORDS NH-12 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2737) AUTHORS Gogolok S, Garcia-Diaz C, Pollard SM TITLE STAR: a simple TAL effector assembly reaction using isothermal assembly. JOURNAL Sci Rep. 2016 Sep 12;6:33209. doi: 10.1038/srep33209. PUBMED 27615025 REFERENCE 2 (bases 1 to 2737) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2737 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 278..300 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 292..309 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 293..309 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 320..339 /label=T7 /note="T7 promoter, forward primer" promoter 320..338 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(880..897) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(990..1578) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1070..1089) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1749..2609) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2372..2391 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2610..2714) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt 181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca 361 aggcctaggc gcgccatgag ctcgtatcct gcagttaacg ccggcccaag tagtcgcaat 421 cgcgtcgaat cacgggggaa agcaagccct ggaaaccgtg caaaggttgt tgccggtatt 481 atgtcaagat catgggcttc agactcgaac aagcgggcgc tgctcgacgc actggccgaa 541 gccatgctgg cggagtcgag aatcatagca cttcggtgcc gagagccgac gacgactggc 601 gctcatttcg agtctgactg ggaatgcccg cagcttcagg caggcgctgc tcgcctaccg 661 ccagcagagt ccaatggatc tcgaggaatt cactcctcag gtgcaggctg cctatcagga 721 gtcccattgt gctggcgcgc tgcaggatac ggtacctctt aattaactgg cctcatgggc 781 cttccgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaacatgg 841 tcatagctgt ttccttgcgt attgggcgct ctccgcttcc tcgctcactg actcgctgcg 901 ctcggtcgtt cgggtaaagc ctggggtgcc taatgagcaa aaggccagca aaaggccagg 961 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 1021 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 1081 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 1141 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 1201 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 1261 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 1321 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 1381 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 1441 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 1501 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 1561 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 1621 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 1681 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 1741 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 1801 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 1861 tctggcccca gtgctgcaat gataccgcga gaaccacgct caccggctcc agatttatca 1921 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 1981 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 2041 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 2101 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 2161 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 2221 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 2281 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 2341 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 2401 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 2461 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 2521 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 2581 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 2641 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 2701 ataggggttc cgcgcacatt tccccgaaaa gtgccac //