LOCUS Exported 2515 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION For library amplification of fragment NH-11. ACCESSION . VERSION . KEYWORDS NH-11 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2515) AUTHORS Gogolok S, Garcia-Diaz C, Pollard SM TITLE STAR: a simple TAL effector assembly reaction using isothermal assembly. JOURNAL Sci Rep. 2016 Sep 12;6:33209. doi: 10.1038/srep33209. PUBMED 27615025 REFERENCE 2 (bases 1 to 2515) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2515 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 278..300 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 292..309 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 293..309 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 320..339 /label=T7 /note="T7 promoter, forward primer" promoter 320..338 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(658..675) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(768..1356) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(848..867) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1527..2387) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2150..2169 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2388..2492) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt 181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca 361 aggcctaggc gcgccatgag ctcgtatcct gcagttaacg cccgaccagg ttgttgcaat 421 cgcgtcgaat cacgggggaa agcaagccct ggaaaccgtg caaaggttgt tgccggtgtt 481 gtgccaagcg cacggcttaa cgccggccca agtagtcctg caggatacgg tacctcttaa 541 ttaactggcc tcatgggcct tccgctcact gcccgctttc cagtcgggaa acctgtcgtg 601 ccagctgcat taacatggtc atagctgttt ccttgcgtat tgggcgctct ccgcttcctc 661 gctcactgac tcgctgcgct cggtcgttcg ggtaaagcct ggggtgccta atgagcaaaa 721 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 781 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 841 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 901 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 961 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 1021 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 1081 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 1141 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 1201 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 1261 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 1321 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 1381 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 1441 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 1501 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 1561 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 1621 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga accacgctca 1681 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 1741 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 1801 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 1861 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 1921 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 1981 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 2041 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 2101 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 2161 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 2221 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 2281 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 2341 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 2401 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 2461 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccac //