LOCUS Exported 6975 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Pmyo-3-PH::miniSOG, expression of membrane targeted PH-miniSOG in body wall muscles.. ACCESSION . VERSION . KEYWORDS pCZGY2838 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6975) AUTHORS Xu S, Chisholm AD TITLE Highly efficient optogenetic cell ablation in C. elegans using membrane-targeted miniSOG. JOURNAL Sci Rep. 2016 Feb 10;6:21271. doi: 10.1038/srep21271. PUBMED 26861262 REFERENCE 2 (bases 1 to 6975) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6975 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 444..761 /codon_start=1 /product="mini singlet oxygen generator, a fluorescent flavoprotein suitable for correlated light and electron microscopy (Shu et al., 2011)" /label=miniSOG /translation="EKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQGP ETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQLD G" protein_bind complement(785..809) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" primer_bind complement(835..851) /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(835..851) /label=pBluescriptSK /note="For pBluescript vector" primer_bind complement(1588..1608) /label=T3 /note="T3 promoter, forward primer" promoter complement(1588..1606) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(1627..1643) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1627..1643) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1640..1662) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 1651..1667 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1675..1705) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 1720..1741 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(1858..1875) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2029..2617) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2109..2128) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2788..3648) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3411..3430 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3649..3753) /gene="bla" /label=AmpR promoter rep_origin complement(3779..4234) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3916..3937) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 4128..4147 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4361..4383 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 4375..4392 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 4376..4392 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 4402..4421 /label=T7 /note="T7 promoter, forward primer" promoter 4402..4420 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 6968..17 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" ORIGIN 1 gtacaaaaaa gcaggctccg aattcgccct tcggatccag atctatgcac gggctccagg 61 atgacccgga ccttcaggcc cttctgaagg gcagccagct tctgaaggtg aagtccagct 121 cgtggcgtag ggaacgcttc tacaagctac aggaggactg caagaccatc tggcaggaat 181 ctcgaaaggt catgaggtcc ccggagtcgc agctgttctc catcgaggac attcaggagg 241 tacggatggg acaccgcaca gaaggcctgg agaagtttgc ccgagacatc cccgaggatc 301 gatgcttctc cattgtcttc aaggaccagc gcaacaccct agacctcatt gccccatcac 361 cagctgacgc tcagcactgg gtgcagggcc tgcgcaagat catccaccac tccggctcca 421 tggaccagcg gcagaagact agtgagaaaa gtttcgtgat aactgatcca cggctgccag 481 acaatcccat catcttcgca tccgatggct tcctggagct gaccgagtat tccagagagg 541 agatcctggg ccgcaatggc cgctttctgc agggaccaga gacagaccag gccacagtgc 601 agaagattcg cgatgccatt agagatcagc gcgagattac cgtgcagctg ataaactaca 661 caaaaagcgg gaagaaattc tggaacctcc tgcacctcca gcccatgagg gaccagaagg 721 gtgagctcca gtatttcatc ggagtgcagc tggatggatg actcgagtaa caagggcgaa 781 ttcgacccag ctttcttgta caaagtggtg atatcgaatt cctgcagccc gggggatcca 841 ctagttctag agcggccgcc accgcggccg ctgtcatcag atcgccatct cgcgcccgtg 901 cctctgactt ctaagtccaa ttactcttca acatccctac atgctctttc tccctgtgct 961 cccaccccct atttttgtta ttatcaaaaa acttctctta atttctttgt tttttagctt 1021 cttttaagtc acctctaaca atgaaattgt gtagattcaa aaatagaatt aattcgtaat 1081 aaaaagtcga aaaaaattgt gctccctccc cccattaata ataattctat cccaaaatct 1141 acacaatgtt ctgtgtacac ttcttatgtt ttttacttct gataaatttt tttgaaacat 1201 catagaaaaa accgcacaca aaatacctta tcatatgtta cgtttcagtt tatgaccgca 1261 atttttattt cttcgcacgt ctgggcctct catgacgtca aatcatgctc atcgtgaaaa 1321 agttttggag tatttttgga atttttcaat caagtgaaag tttatgaaat taattttcct 1381 gcttttgctt tttggggttt cccctattgt ttgtcaagat ttcgaggacg gcgtttttct 1441 tgctaaaatc acaagtattg atgagcacga tgcaagaaag atcggaagaa ggtttgggtt 1501 tgaggctcag tggaaggtga gtagaagttg ataatttgaa agtggagtag tgtctatggg 1561 gtttttgccg agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta 1621 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 1681 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 1741 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 1801 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 1861 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 1921 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 1981 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 2041 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 2101 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 2161 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 2221 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 2281 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 2341 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 2401 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 2461 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 2521 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 2581 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 2641 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 2701 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 2761 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 2821 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 2881 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 2941 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 3001 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 3061 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 3121 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 3181 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 3241 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 3301 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 3361 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 3421 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 3481 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 3541 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 3601 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 3661 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 3721 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa 3781 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt 3841 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata 3901 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac 3961 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa 4021 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc 4081 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg 4141 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca 4201 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac 4261 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga 4321 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa 4381 acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg taccgtcggc 4441 tataataagt tcttgaataa aataattttc ccgacaaaac atgagtattt ctttcgaaaa 4501 taaaagtgca ggctaattag agattattct gtaattaact gcataatttg tcacgtgcca 4561 tagttttaca ttccactacg tcatagttct taaaatacta atctcctgaa aatagaagta 4621 ggtgaagaaa gtttaattat cagttctaaa atgacaattg atctttggaa tatgttctga 4681 aactaccgat cattgaacag atgctatttg aatgatatag aattgtatat ttgcaatttc 4741 tgaaacgcgt tcttaaaggc acacagatta attcaaaagg gtctggccgc aaaaaggttt 4801 atggtggccg attttgagtt ttgtgtgtga ttgctttttc acaatcagtg ttttcaggat 4861 tatgtgatga actagatctt caagtttcgt tacatttcat atgttttcgg aactcacgaa 4921 gtacatattg ggtattgtgc tcaaaaaatt cagcaatcag cttcgctccg ctgactttag 4981 aacccaaaaa aatagtatgg ccaaactgac tgtgttacga tcatttcaat ttttcaatac 5041 atatttaaga tttctaagag taagaaggtc aaaaactgtt ctggaataca tatatatttt 5101 tcaggttaca attagtcaaa aagtgcactg aaatatacgt tttaatttca cgaataaccc 5161 aattagttca atgtattttt ggtcaaccaa cgttaaagtt tggcttccaa ccaattatca 5221 tttctgatca accacaatgt tttttcttta tctgcaagtt aattttttat ttttatccag 5281 atgtttggca tatttttcaa ttcttcacta gcgcccactt cttgcacttc cggcgccctg 5341 aatctaatgc atctgttgca agaattgaaa gaccaatcaa cacattgttt tcttcacgag 5401 atactgaaga aaatgaataa aaacagagaa aaagagccat gtgattagtg acaactgttg 5461 ctaacagata ccatagcttg gacttggtac gtgatggcaa cgtatgggtc aacaaaaatg 5521 attgcagagg gggtgcaaaa cagtcaagtc gagaaaatat gaaaaacaga aaacaaagaa 5581 cagaaaaatg ggtttgagag tcagtataat ttataaaaga aaaattgtac atagaaatta 5641 accatttttg tagaagaagt tatttttcaa gcatcgttaa aaattattca aagcacctta 5701 tttcatattt aattttaaac atggttaaat gaacaacacg gtgcgcaatc aggaaaactt 5761 gaaatctgaa actgttgttg tgatcttctt cgcaactgtt cagatagcac tagtgtaatg 5821 ttaagagtgc gcgaatataa tggaatataa tggatcacac ctcctgccat caggtaaacg 5881 tctctgttat cacatatttc caactattaa atttttacct tttacagttt tacatttttt 5941 tgaaaaaagt aactttttgt cttcaaaatc cctgacgaaa atatcaaata ttttaatcga 6001 gactgcagag gaaccgattg atgatttgga aaatccagct ttacctgtgt aagaactgaa 6061 aagtttcata accctagggt attcccagtt acattcccca ctggctaaca atagcaccca 6121 gtttttcatc acctttcttc aaatttctcg gcgatttgtt aaaaacaaaa tttgtgtccc 6181 ttctctgata tctctatgtc tctaaacaca agttcatcgg aaaacgaagg agggtaggtg 6241 ttggttgggc tcccgaagtg aaaatagaag agcaagaata gaatattaga gagagagtgc 6301 agagagggcg ggatagctcc cgggattccg ttttcttctt ctttatcttc aacgatgatg 6361 tgtgtgcgtg ttgtatagat tctgttgctc ccccacaact cgctccgaag gctcaataca 6421 attcaattga tattggagga gagcctaccg gagtgggagg ataagaagaa acataagaag 6481 aagaagaaga agaagcatgc ttctggtttt tgatgctatg aaaacggcac aaaaagatga 6541 ttgaggtccc ttttcaatac cttctctcat ctttcaaatc ccattgaaac ctaaaacttc 6601 tcaccacgct ttaccattgt tctccaaaaa cttatagcaa tgtctataac ttttttatct 6661 ctgaaaagca gtgttccatt tttctttttc ctattttatt tcaattgttt ctcacatttc 6721 gtttggattc tttgcttgtc aaccagcttc ttcttccact tttaccgtct aattttcagg 6781 gcagggagcc atcaaaccca cgaccactag atccatctag aaaagcttgc atgcctgcag 6841 gtcgactcta gaggatcccc gggattggcc aaaggaccca aaggtatgtt tcgaatgata 6901 ctaacataac atagaacatt ttcaggagga cccttggagg gtaccggtag aaaaaaagct 6961 tgatatcaca agttt //