LOCUS       Exported                7730 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  mammalian expression of human androgen receptor in pCMV5.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-hAR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   Lubahn DB, Joseph DR, Sullivan PM, Willard HF, French FS, Wilson EM
  TITLE     Cloning of human androgen receptor complementary DNA and 
            localization to the X chromosome.
  JOURNAL   Science. 1988 Apr 15;240(4850):327-30.
  PUBMED    3353727
REFERENCE   2  (bases 1 to 7730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7730
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    3754..4376
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3953..3972)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(4400..4420)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4405..4734
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4585..4720
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4647..4666
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(4772..4791)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4773..4791)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4805..4821)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4805..4821)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4818..4840)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4829..4845
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4853..4883)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4898..4919
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5036..5053)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5207..5795)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5287..5306)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5966..6826)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6589..6608
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6827..6931)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6958..7413
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7045..7064)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7255..7276
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7539..7561
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7553..7570
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7554..7570
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agaattcaga tctgtccacc gtgtgtcttc ttctgcacga gactttgagg ctgtcagagc
      661 gctttttgcg tggttgctcc cgcaagtttc cttctctgga gcttcccgca ggtgggcagc
      721 tagctgcagc gactaccgca tcatcacagc ctgttgaact cttctgagca agagaagggg
      781 aggcggggta agggaagtag gtggaagatt cagccaagct caaggatgga agtgcagtta
      841 gggctgggaa gggtctaccc tcggccgccg tccaagacct accgaggagc tttccagaat
      901 ctgttccaga gcgtgcgcga agtgatccag aacccgggcc ccaggcaccc agaggccgcg
      961 agcgcagcac ctcccggcgc cagtttgctg ctgctgcagc agcagcagca gcagcagcag
     1021 cagcagcagc agcagcagca gcagcagcag cagcagcagc agcaagagac tagccccagg
     1081 cagcagcagc agcagcaggg tgaggatggt tctccccaag cccatcgtag aggccccaca
     1141 ggctacctgg tcctggatga ggaacagcaa ccttcacagc cgcagtcggc cctggagtgc
     1201 caccccgaga gaggttgcgt cccagagcct ggagccgccg tggccgccag caaggggctg
     1261 ccgcagcagc tgccagcacc tccggacgag gatgactcag ctgccccatc cacgttgtcc
     1321 ctgctgggcc ccactttccc cggcttaagc agctgctccg ctgaccttaa agacatcctg
     1381 agcgaggcca gcaccatgca actccttcag caacagcagc aggaagcagt atccgaaggc
     1441 agcagcagcg ggagagcgag ggaggcctcg ggggctccca cttcctccaa ggacaattac
     1501 ttagggggca cttcgaccat ttctgacaac gccaaggagt tgtgtaaggc agtgtcggtg
     1561 tccatgggcc tgggtgtgga ggcgttggag catctgagtc caggggaaca gcttcggggg
     1621 gattgcatgt acgccccact tttgggagtt ccacccgctg tgcgtcccac tccttgtgcc
     1681 ccattggccg aatgcaaagg ttctctgcta gacgacagcg caggcaagag cactgaagat
     1741 actgctgagt attccccttt caagggaggt tacaccaaag ggctagaagg cgagagccta
     1801 ggctgctctg gcagcgctgc agcagggagc tccgggacac ttgaactgcc gtctaccctg
     1861 tctctctaca agtccggagc actggacgag gcagctgcgt accagagtcg cgactactac
     1921 aactttccac tggctctggc cggaccgccg ccccctccgc cgcctcccca tccccacgct
     1981 cgcatcaagc tggagaaccc gctggactac ggcagcgcct gggcggctgc ggcggcgcag
     2041 tgccgctatg gggacctggc gagcctgcat ggcgcgggtg cagcgggacc cggttctggg
     2101 tcaccctcag ccgccgcttc ctcatcctgg cacactctct tcacagccga agaaggccag
     2161 ttgtatggac cgtgtggtgg tggtgggggt ggtggcggcg gcggcggcgg cggcggcggc
     2221 ggcggcggcg gcggcggcgg cggcgaggcg ggagctgtag ccccctacgg ctacactcgg
     2281 ccccctcagg ggctggcggg ccaggaaagc gacttcaccg cacctgatgt gtggtaccct
     2341 ggcggcatgg tgagcagagt gccctatccc agtcccactt gtgtcaaaag cgaaatgggc
     2401 ccctggatgg atagctactc cggaccttac ggggacatgc gtttggagac tgccagggac
     2461 catgttttgc ccattgacta ttactttcca ccccagaaga cctgcctgat ctgtggagat
     2521 gaagcttctg ggtgtcacta tggagctctc acatgtggaa gctgcaaggt cttcttcaaa
     2581 agagccgctg aagggaaaca gaagtacctg tgcgccagca gaaatgattg cactattgat
     2641 aaattccgaa ggaaaaattg tccatcttgt cgtcttcgga aatgttatga agcagggatg
     2701 actctgggag cccggaagct gaagaaactt ggtaatctga aactacagga ggaaggagag
     2761 gcttccagca ccaccagccc cactgaggag acaacccaga agctgacagt gtcacacatt
     2821 gaaggctatg aatgtcagcc catctttctg aatgtcctgg aagccattga gccaggtgta
     2881 gtgtgtgctg gacacgacaa caaccagccc gactcctttg cagccttgct ctctagcctc
     2941 aatgaactgg gagagagaca gcttgtacac gtggtcaagt gggccaaggc cttgcctggc
     3001 ttccgcaact tacacgtgga cgaccagatg gctgtcattc agtactcctg gatggggctc
     3061 atggtgtttg ccatgggctg gcgatccttc accaatgtca actccaggat gctctacttc
     3121 gcccctgatc tggttttcaa tgagtaccgc atgcacaagt cccggatgta cagccagtgt
     3181 gtccgaatga ggcacctctc tcaagagttt ggatggctcc aaatcacccc ccaggaattc
     3241 ctgtgcatga aagcactgct actcttcagc attattccag tggatgggct gaaaaatcaa
     3301 aaattctttg atgaacttcg aatgaactac atcaaggaac tcgatcgtat cattgcatgc
     3361 aaaagaaaaa atcccacatc ctgctcaaga cgcttctacc agctcaccaa gctcctggac
     3421 tccgtgcagc ctattgcgag agagctgcat cagttcactt ttgacctgct aatcaagtca
     3481 cacatggtga gcgtggactt tccggaaatg atggcagaga tcatctctgt gcaagtgccc
     3541 aagatccttt ctgggaaagt caagcccatc tatttccaca cccagtgaag cattggaaac
     3601 cctatttccc caccccagct catgccccct ttcagatgtc ttctgcctgt tataactctg
     3661 cactactcct ctgcagtgcc ttggggaatt tcctctattg atgtacagtc tgtcatgagc
     3721 ttgcatgcct gcaggtcgac tctagaggat cccgggtggc atccctgtga cccctcccca
     3781 gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa
     3841 ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg
     3901 gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg
     3961 gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt
     4021 caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg
     4081 ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc
     4141 tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag
     4201 gcgtgaacca ctgctccctt ccctgtcctt ctgattttaa aataactata ccagcaggag
     4261 gacgtccaga cacagcatag gctacctggc catgcccaac cggtgggaca tttgagttgc
     4321 ttgcttggca ctgtcctctc atgcgttggg tccactcagt agatgcctgt tgaattgggt
     4381 acgcggccag cttggctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     4441 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     4501 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4561 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     4621 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     4681 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     4741 ctcgaggaac tgaaaaacca gaaagttaat tccctatagt gagtcgtatt aaattcgtaa
     4801 tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     4861 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     4921 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     4981 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     5041 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     5101 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     5161 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     5221 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     5281 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     5341 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     5401 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     5461 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5521 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     5581 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     5641 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     5701 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     5761 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     5821 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     5881 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     5941 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     6001 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     6061 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     6121 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     6181 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     6241 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     6301 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     6361 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     6421 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     6481 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     6541 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     6601 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     6661 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     6721 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     6781 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     6841 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     6901 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     6961 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     7021 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     7081 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     7141 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     7201 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     7261 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     7321 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     7381 cgaattttaa caaaatatta acgcttacaa tttccattcg ccattcaggc tgcgcaactg
     7441 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     7501 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     7561 gacggccagt gccaagctga tctatacatt gaatcaatat tggcaattag ccatattagt
     7621 cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt tgtatctata
     7681 tcataatatg tacatttata ttggctcatg tccaatatga ccgccatgtt
//
