LOCUS Exported 8898 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Hsp68-LacZ-Gateway SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8898) AUTHORS Pennacchio LA, Ahituv N, Moses AM, Prabhakar S, Nobrega MA, Shoukry M, Minovitsky S, Dubchak I, Holt A, Lewis KD, Plajzer-Frick I, Akiyama J, De Val S, Afzal V, Black BL, Couronne O, Eisen MB, Visel A, Rubin EM TITLE In vivo enhancer analysis of human conserved non-coding sequences. JOURNAL Nature. 2006 Nov 23;444(7118):499-502. Epub 2006 Nov 5. PUBMED 17086198 REFERENCE 2 (bases 1 to 8898) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8898 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(77..382) /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind complement(179..198) /label=ccdB-fwd /note="ccdB gene, forward primer" CDS complement(724..1383) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 1298..1317 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" promoter complement(1437..1467) /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" protein_bind complement(1492..1616) /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1641..1657) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1642..1658) /label=pBluescriptKS /note="For pBluescript vector" promoter complement(1686..1704) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(1725..1741) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1725..1741) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1738..1760) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 1749..1765 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1773..1803) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 1818..1839 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(1956..1973) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2127..2715) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2207..2226) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2886..3746) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3509..3528 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3747..3851) /gene="bla" /label=AmpR promoter rep_origin 3878..4333 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3965..3984) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4175..4196 /label=F1ori-F /note="F1 origin, forward primer" primer_bind 4473..4490 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 4474..4490 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 4497..4516 /label=T7 /note="T7 promoter, forward primer" promoter 4497..4515 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind 4551..4567 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind 4551..4567 /label=pBluescriptSK /note="For pBluescript vector" polyA_signal 4564..4698 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4588..4607) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 4642..4661 /label=SV40pA-R /note="SV40 polyA, reverse primer" CDS complement(4846..7893) /codon_start=1 /gene="lacZ" /product="beta-galactosidase" /label=lacZ /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD SWSPSVSAEFQLSAGRYHYQLVWCQK" protein_bind 8810..36 /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" ORIGIN 1 ataatactgt aaaacacaac atatccagtc actatggtcg acctgcagac tggctgtgta 61 taagggagcc tgacatttat attccccaga acatcaggtt aatggcgttt ttgatgtcat 121 tttcgcggtg gctgagatca gccacttctt ccccgataac ggagaccggc acactggcca 181 tatcggtggt catcatgcgc cagctttcat ccccgatatg caccaccggg taaagttcac 241 gggagacttt atctgacagc agacgtgcac tggccagggg gatcaccatc cgtcgcccgg 301 gcgtgtcaat aatatcactc tgtacatcca caaacagacg ataacggctc tctcttttat 361 aggtgtaaac cttaaactgc atttcaccag cccctgttct cgtcagcaaa agagccgttc 421 atttcaataa accgggcgac ctcagccatc ccttcctgat tttccgcttt ccagcgttcg 481 gcacgcagac gacgggcttc attctgcatg gttgtgctta ccagaccgga gatattgaca 541 tcatatatgc cttgagcaac tgatagctgt cgctgtcaac tgtcactgta atacgctgct 601 tcatagcata cctctttttg acatacttcg ggtatacata tcagtatata ttcttatacc 661 gcaaaaatca gcgcgcaaat acgcatactg ttatctggct tttagtaagc cggatccacg 721 cgtttacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc 781 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt 841 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt 901 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat 961 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg 1021 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa 1081 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag 1141 ctcaccgtct ttcattgcca tacggaattc cggatgagca ttcatcaggc gggcaagaat 1201 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt 1261 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa 1321 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc 1381 cattttagct tccttagctc ctgaaaatct cgacggatcc taactcaaaa tccacacatt 1441 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgcggccg ccatagtgac 1501 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat 1561 atattgatat ttatatcatt ttacgtttct cgttcagctt ttttgtacaa acttgtgatg 1621 gaattcgata tcaagcttat cgataccgtc gacctcgagg gggggcccgg taccagcttt 1681 tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata gctgtttcct 1741 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 1801 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 1861 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 1921 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 1981 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 2041 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 2101 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 2161 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 2221 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 2281 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 2341 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 2401 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 2461 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 2521 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 2581 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 2641 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 2701 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 2761 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 2821 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 2881 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 2941 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 3001 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 3061 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 3121 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 3181 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 3241 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 3301 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 3361 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 3421 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 3481 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 3541 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 3601 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 3661 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 3721 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 3781 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 3841 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta 3901 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 3961 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 4021 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 4081 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 4141 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 4201 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 4261 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta 4321 acgcttacaa tttccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc 4381 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt 4441 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat 4501 acgactcact atagggcgaa ttggcgtcga cgcaccgcgg tggcggccgc tctagaacta 4561 gtggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag 4621 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata 4681 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg 4741 gaggtgtggg aggtttttta aagcaagtaa aacctctaca gatgtgatat ggctgattat 4801 gatcattact tatctagagc ttagatcccc cctgcccggt tattattatt tttgacacca 4861 gaccaactgg taatggtagc gaccggcgct cagctggaat tccgccgata ctgacgggct 4921 ccaggagtcg tcgccaccaa tccccatatg gaaaccgtcg atattcagcc atgtgccttc 4981 ttccgcgtgc agcagatggc gatggctggt ttccatcagt tgctgttgac tgtagcggct 5041 gatgttgaac tggaagtcgc cgcgccactg gtgtgggcca taattcaatt cgcgcgtccc 5101 gcagcgcaga ccgttttcgc tcgggaagac gtacggggta tacatgtctg acaatggcag 5161 atcccagcgg tcaaaacagg cggcagtaag gcggtcggga tagttttctt gcggccctaa 5221 tccgagccag tttacccgct ctgctacctg cgccagctgg cagttcaggc caatccgcgc 5281 cggatgcggt gtatcgctcg ccacttcaac atcaacggta atcgccattt gaccactacc 5341 atcaatccgg taggttttcc ggctgataaa taaggttttc ccctgatgct gccacgcgtg 5401 agcggtcgta atcagcaccg catcagcaag tgtatctgcc gtgcactgca acaacgctgc 5461 ttcggcctgg taatggcccg ccgccttcca gcgttcgacc caggcgttag ggtcaatgcg 5521 ggtcgcttca cttacgccaa tgtcgttatc cagcggtgca cgggtgaact gatcgcgcag 5581 cggcgtcagc agttgttttt tatcgccaat ccacatctgt gaaagaaagc ctgactggcg 5641 gttaaattgc caacgcttat tacccagctc gatgcaaaaa tccatttcgc tggtggtcag 5701 atgcgggatg gcgtgggacg cggcggggag cgtcacactg aggttttccg ccagacgcca 5761 ctgctgccag gcgctgatgt gcccggcttc tgaccatgcg gtcgcgttcg gttgcactac 5821 gcgtactgtg agccagagtt gcccggcgct ctccggctgc ggtagttcag gcagttcaat 5881 caactgttta ccttgtggag cgacatccag aggcacttca ccgcttgcca gcggcttacc 5941 atccagcgcc accatccagt gcaggagctc gttatcgcta tgacggaaca ggtattcgct 6001 ggtcacttcg atggtttgcc cggataaacg gaactggaaa aactgctgct ggtgttttgc 6061 ttccgtcagc gctggatgcg gcgtgcggtc ggcaaagacc agaccgttca tacagaactg 6121 gcgatcgttc ggcgtatcgc caaaatcacc gccgtaagcc gaccacgggt tgccgttttc 6181 atcatattta atcagcgact gatccaccca gtcccagacg aagccgccct gtaaacgggg 6241 atactgacga aacgcctgcc agtatttagc gaaaccgcca agactgttac ccatcgcgtg 6301 ggcgtattcg caaaggatca gcgggcgcgt ctctccaggt agcgaaagcc attttttgat 6361 ggaccatttc ggcacagccg ggaagggctg gtcttcatcc acgcgcgcgt acatcgggca 6421 aataatatcg gtggccgtgg tgtcggctcc gccgccttca tactgcaccg ggcgggaagg 6481 atcgacagat ttgatccagc gatacagcgc gtcgtgatta gcgccgtggc ctgattcatt 6541 ccccagcgac cagatgatca cactcgggtg attacgatcg cgctgcacca ttcgcgttac 6601 gcgttcgctc atcgccggta gccagcgcgg atcatcggtc agacgattca ttggcaccat 6661 gccgtgggtt tcaatattgg cttcatccac cacatacagg ccgtagcggt cgcacagcgt 6721 gtaccacagc ggatggttcg gataatgcga acagcgcacg gcgttaaagt tgttctgctt 6781 catcagcagg atatcctgca ccatcgtctg ctcatccatg acctgaccat gcagaggatg 6841 atgctcgtga cggttaacgc ctcgaatcag caacggcttg ccgttcagca gcagcagacc 6901 attttcaatc cgcacctcgc ggaaaccgac atcgcaggct tctgcttcaa tcagcgtgcc 6961 gtcggcggtg tgcagttcaa ccaccgcacg atagagattc gggatttcgg cgctccacag 7021 tttcgggttt tcgacgttca gacgtagtgt gacgcgatcg gcataaccac cacgctcatc 7081 gataatttca ccgccgaaag gcgcggtgcc gctggcgacc tgcgtttcac cctgccataa 7141 agaaactgtt acccgtaggt agtcacgcaa ctcgccgcac atctgaactt cagcctccag 7201 tacagcgcgg ctgaaatcat cattaaagcg agtggcaaca tggaaatcgc tgatttgtgt 7261 agtcggttta tgcagcaacg agacgtcacg gaaaatgccg ctcatccgcc acatatcctg 7321 atcttccaga taactgccgt cactccagcg cagcaccatc accgcgaggc ggttttctcc 7381 ggcgcgtaaa aatgcgctca ggtcaaattc agacggcaaa cgactgtcct ggccgtaacc 7441 gacccagcgc ccgttgcacc acagatgaaa cgccgagtta acgccatcaa aaataattcg 7501 cgtctggcct tcctgtagcc agctttcatc aacattaaat gtgagcgagt aacaacccgt 7561 cggattctcc gtgggaacaa acggcggatt gaccgtaatg ggataggtca cgttggtgta 7621 gatgggcgca tcgtaaccgt gcatctgcca gtttgagggg acgacgacag tatcggcctc 7681 aggaagatcg cactccagcc agctttccgg caccgcttct ggtgccggaa accaggcaaa 7741 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 7801 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc 7861 agggttttcc cagtcacgac gttgtaaaac gacgggatca tcgcgagcca tggcgccgcg 7921 ctctgcttct ggaaggctgc gctccgcggc gtggatgctc cggggaaagt tgcgctccgc 7981 ggcagggatg ctcctgggaa ggttgcgctc cgcggcaggg atgctctggg gaaggctggt 8041 cctggccgag gatcgggaac gcgccgctcg ctctgcttct cttgtcttcg cttgtctctg 8101 gatggaacca gatttggttc tgagtagctg tcagcgtctg gtgacctgct cgccgccctg 8161 cgcctttaag gagtcttcac cggccccgcc cactctccgc tgggccaatc agcgagccgg 8221 aggaggcctt ggggccagga atcttccagc agtttcgcgt ctggtggagc ttccccgcct 8281 cccttgagta atcggagttg tgggttccgc ccttgtccag aactctccag aggtttctgg 8341 ggttcactgg agagtacgga ttcctgaggg ggagggtgtg gggaagtgct ggtgctacta 8401 gtgacactgt tgctatggcg acgcattact aaggcctgtg tggaatggac aagaaagatc 8461 acctctagct cggtgttgtg tacagtttgt tgtgatttgt ggggtttcgc caactcgcac 8521 agttctgaat atgggggtta aaggctaaaa cttaagggct aaaacttctc cccgccaagt 8581 ttaggagacc cagggagatg cctgggggcg tgtccggtga cgtgatcctc tccaatcgcg 8641 ttacaatggc agtgctgcct ctgacctcat ggactaattt aggaactaga ggctctgtcc 8701 cagcacaggc tcaaagttgc cgggaggggc ggggtggggg gtggggggga ccccggctgc 8761 tcagtttgga tgttcctgga gctcggtacc cggggatccc ccgggcatca ccactttgta 8821 caagaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt 8881 gcataaaaaa cagactac //