LOCUS Exported 5257 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: Membrane, Excitation: 587, Emission: 610. ACCESSION . VERSION . KEYWORDS mCherry-Caveolin-C-10 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5257) AUTHORS Hanson CA, Drake KR, Baird MA, Han B, Kraft LJ, Davidson MW, Kenworthy AK TITLE Overexpression of caveolin-1 is sufficient to phenocopy the behavior of a disease-associated mutant. JOURNAL Traffic. 2013 Jun;14(6):663-77. doi: 10.1111/tra.12066. Epub 2013 Apr 7. PUBMED 23469926 REFERENCE 2 (bases 1 to 5257) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5257 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 61..364 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 519..539 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 607..616 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 613..1320 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(758..776) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 1026..1045 /label=mCherry-F /note="mCherry, forward primer" polyA_signal 2045..2166 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2082..2101) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2136..2155 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2173..2628) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2310..2331) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2522..2541 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2655..2759 /gene="bla" /label=AmpR promoter promoter 2761..3118 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2784..2804) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2969..3104 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3031..3050 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3153..3947 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3207..3226) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3817..3836 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4135..4154) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4179..4226 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4555..5143 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5044..5063 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 1321 tccggactca gatctggcag cagtggaggt tctgggggca aatacgtaga ctcggaggga 1381 catctctaca ccgttcccat ccgggaacag ggcaacatct acaagcccaa caacaaggcc 1441 atggcagacg agctgagcga gaagcaagtg tacgacgcgc acaccaagga gatcgacctg 1501 gtcaaccgcg accctaaaca cctcaacgat gacgtggtca agattgactt tgaagatgtg 1561 attgcagaac cagaagggac acacagtttt gacggcattt ggaaggccag cttcaccacc 1621 ttcactgtga cgaaatactg gttttaccgc ttgctgtctg ccctctttgg catcccgatg 1681 gcactcatct ggggcattta cttcgccatt ctctctttcc tgcacatctg ggcagttgta 1741 ccatgcatta agagcttcct gattgagatt cagtgcatca gccgtgtcta ttccatctac 1801 gtccacaccg tctgtgaccc actctttgaa gctgttggga aaatattcag caatgtccgc 1861 atcaacttgc agaaagaaat ataagaattc tgcagtcgac ggtaccgcgg gcccgggatc 1921 caccggatct agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc 1981 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt 2041 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 2101 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 2161 atcttaacgc gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt 2221 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag 2281 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga 2341 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg 2401 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc 2461 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg 2521 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc 2581 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt 2641 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 2701 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc 2761 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg 2821 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg 2881 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 2941 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 3001 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 3061 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga 3121 tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag 3181 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg 3241 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca 3301 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc 3361 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg 3421 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg 3481 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta 3541 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag 3601 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac 3661 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg 3721 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg 3781 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg 3841 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg 3901 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg 3961 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc 4021 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct 4081 ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac 4141 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa 4201 aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc 4261 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc 4321 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg 4381 ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta 4441 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 4501 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 4561 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 4621 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 4681 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa 4741 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 4801 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 4861 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 4921 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 4981 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 5041 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 5101 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 5161 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 5221 ccctgattct gtggataacc gtattaccgc catgcat //