LOCUS Exported 3657 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Can be used for in situ hybridisation or other purposes. ACCESSION . VERSION . KEYWORDS Rab7a cds in pGem-T Easy SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3657) AUTHORS Hall TE, Martel N, Lo HP, Xiong Z, Parton RG TITLE A plasmid library of full-length zebrafish rab proteins for in vivo cell biology. JOURNAL Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 10.1080/21592799.2017.1301151. eCollection 2017. PUBMED 28396820 REFERENCE 2 (bases 1 to 3657) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3657 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 132..151 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 385..402 /label=L4440 /note="L4440 vector, forward primer" protein_bind 519..540 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 555..585 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 593..609 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 598..620 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 617..633 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 617..633 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" promoter 651..669 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 651..668 /label=SP6 /note="SP6 promoter, forward primer" regulatory 1405..1414 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 1409..1418 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" primer_bind complement(1448..1467) /label=T7 /note="T7 promoter, forward primer" promoter complement(1449..1467) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1474..1491) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(1474..1490) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1483..1505) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 1631..2086 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1718..1737) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 1928..1949 /label=F1ori-F /note="F1 origin, forward primer" promoter 2164..2268 /gene="bla" /label=AmpR promoter CDS 2269..3129 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(2487..2506) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 3300..231 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 61 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 301 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 361 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca 421 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 481 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 541 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 601 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct atttaggtga 661 cactatagaa tactcaagct atgcatccaa cgcgttggga gctctcccat atggtcgacc 721 tgcaggcggc cgcgaattca ctagtgattg gtcctcagca gctacaggtc tctgctgatg 781 gtttggctct atcgttccgg tctagtttaa ttggctcggg gaactcgttg tataactcca 841 cttcagtttc ctgtttgagt gcattgcgcg cgatggtctg gaatgcctgc tctacgttga 901 tggcctcctt tgcactggtc tcaaaatatg ggatgttgtt cttactctga caccaagctt 961 gtgctcgctt agttgttacc tgtctattct ccagatcgat tttgttgccc agcaccacga 1021 aagggaagtt ttctgggtct cgaggactgg cctggatcag aaactcatcc ctccagctgt 1081 ccagtgtctt gaaggtgttc ggggcagtga cgtcaaacac caggacgcag cagtcagcac 1141 cacgatagaa agctacaccc agagactgaa accgctcctg acctgctgtg ccccatatct 1201 gcattgtgac aagccggtcg tcaaccatca cttcttttgt caggaaatct gctcctatag 1261 tggctttata ctggttactg aactttttat tcacatactg gttcattaga gatgtttttc 1321 caaccccgga atctccaaga atgatcacct tcagaagaac tttcttcctt gatgtcatag 1381 tgtcagagca atcgaattcc cgcggccgcc atggcggccg ggagcatgcg acgtcgggcc 1441 caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga 1501 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag 1561 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa 1621 tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 1681 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 1741 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 1801 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 1861 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 1921 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 1981 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 2041 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt 2101 ttctccttac gcatctgtgc ggtatttcac accgcatcag gtggcacttt tcggggaaat 2161 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 2221 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 2281 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 2341 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 2401 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 2461 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 2521 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 2581 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 2641 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 2701 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 2761 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 2821 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 2881 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 2941 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 3001 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 3061 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 3121 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 3181 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 3241 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 3301 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 3361 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 3421 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc 3481 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 3541 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 3601 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacg //