LOCUS Exported 10934 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Vector for reporter assay of one zebrafish super-enhancer region. ACCESSION . VERSION . KEYWORDS E1b-GFP-Tol2-Gateway dre SE-zic2a P SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10934) AUTHORS Perez-Rico YA, Boeva V, Mallory AC, Bitetti A, Majello S, Barillot E, Shkumatava A TITLE Comparative analyses of super-enhancers reveal conserved elements in vertebrate genomes. JOURNAL Genome Res. 2017 Feb;27(2):259-268. doi: 10.1101/gr.203679.115. Epub 2016 Dec 13. PUBMED 27965291 REFERENCE 2 (bases 1 to 10934) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10934 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..125 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 150..180 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 234..893 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(300..319) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 1235..1540 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 1419..1438 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(1581..1705) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" intron 3426..3998 /label=-beta-globin intron /note="intron from rabbit beta-globin gene" regulatory 4072..4081 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 4078..4797 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 4078..4794 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(4123..4144) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(4384..4403) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 4731..4752 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 4842..4976 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4866..4885) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 4920..4939 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(5646..5662) /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5646..5662) /label=pBluescriptSK /note="For pBluescript vector" primer_bind complement(5699..5719) /label=T3 /note="T3 promoter, forward primer" promoter complement(5699..5717) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(5738..5754) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5738..5754) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(5751..5773) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 5762..5778 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5786..5816) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5831..5852 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(5969..5986) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6140..6728) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6220..6239) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6899..7759) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 7522..7541 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(7760..7864) /gene="bla" /label=AmpR promoter rep_origin complement(7890..8345) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8027..8048) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 8239..8258 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8472..8494 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 8486..8503 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 8487..8503 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 8513..8532 /label=T7 /note="T7 promoter, forward primer" promoter 8513..8531 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta 61 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca 121 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg 181 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga 241 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg 301 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg 361 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc 421 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg 481 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt 541 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag 601 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt 661 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata 721 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg 781 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca 841 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg 901 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta 961 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca 1021 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc 1081 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa 1141 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac 1201 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa 1261 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg 1321 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga 1381 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc 1441 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga 1501 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca 1561 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag 1621 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc 1681 gttcagcttt cttgtacaaa gtggtgatat caagatctca ccgctggtct tgacatgtta 1741 aaaaggccat tttcatcttt tgtcccgaga aaagcaggag gaaaggactc tctgtagctg 1801 taaataactg taaacatcca ggtaatagag gctcttttgt gtttcttggc catggttgtt 1861 accttgttat ctctagcttt tatgttaata atcagacact caccatcttt gccaactgcg 1921 tgggttacct ttttgtatgc gcaatcgcta ttttgtcgag cttgttgaaa agggggtgaa 1981 tctctccccc caaaaaaggg agggctatct ttgccctgtt acctgagggg attttacccc 2041 accctgccca attttcacct acctctgttt actcacagtc ctcactgtat gtcacaacat 2101 ttacatgtct ttccttttgc ttcaatttgt atgtgttaga tagaaaaaaa attgagcaat 2161 ggaaaaaaat ccctaataga agagcagtcc cctgcaactg tgaaaacccc tccgctggtc 2221 agccaatggc cattcacggg agaacaaaca aaaatcaaat gggagctatg cttttagctt 2281 ttaattccag ccccaaaagt ttccagctgc ttccgtaatt attacctttt aaagagtagg 2341 aaaatggcgg gttaattcac atggtggctt tctctatctt tttgtctttt tctcccttac 2401 tccctgcctc attctctctt tcctcctctc tccaccccaa aaccacaaca ggtaagctct 2461 actgttaaac gagggccttt tttagttgta atttggtttg aaaaccctct ggcttgccca 2521 gattattctt agtcgtttct ctgtttcctg tcggtatgaa ggaggtgcac cttgtgctca 2581 gggagaggaa caatacagcc ttgtcccagg ttcctctgac cccctgcgct gcctgtcagt 2641 ctgtgattgt catgcaaggc cctctctaga aacaaaaggc ctagatgggc tttgtggccc 2701 accaggaccc cagcattgtt attgggatcc ctgtgagtat ttgctcttga gtctgtttct 2761 caacctgaaa gaccttgatg ctgatggttt tgttaatcta agatgtaacg tttaaggtta 2821 tcttgtttta gttgtggggt tgatttacgt caacatcgtt ataagccata caaaaagtaa 2881 gtcattttaa tatatttagc atgtgattca ggtggatagt atgtatctgc attgccttgt 2941 tactgttact gtagccactg catatcagtg tgaattaaag gtttggggtc agtacatgta 3001 catgaggaga cgtagccctg cgctattgtt aaagctcggg tgatggatgg ctgtggtcgc 3061 ccatgatccc agcccctgct ggataggcta tggaaaaact cagcttcata ttgtccttgt 3121 tctgtgtgac aatgggccac ttgtgggtct gacactcatc acctgttagt cggcctcatt 3181 cgtatgaaaa taccacgctg ccttggttcc agcactgtag gtgctttccc tccagccttg 3241 tatgtttgtt tagacaatag tgcccatcac aaagtgacac ttgtaattga gagcttttca 3301 tatcaagctg aggtggatgt gtttcagatc tctcgactct agagggtata taatggatcc 3361 catcgcgtct cagcctcact ttgagctcct ccacacgaat ttcgaccgat cctgagaact 3421 tcagggtgag tttggggacc cttgattgtt ctttcttttt cgctattgta aaattcatgt 3481 tatatggagg gggcaaagtt ttcagggtgt tgtttagaat gggaagatgt cccttgtatc 3541 accatggacc ctcatgataa ttttgtttct ttcactttct actctgttga caaccattgt 3601 ctcctcttat tttcttttca ttttctgtaa ctttttcgtt aaactttagc ttgcatttgt 3661 aacgaatttt taaattcact tttgtttatt tgtcagattg taagtacttt ctctaatcac 3721 ttttttttca aggcaatcag ggtatattat attgtacttc agcacagttt tagagaacaa 3781 ttgttataat taaatgataa ggtagaatat ttctgcatat aaattctggc tggcgtggaa 3841 atattcttat tggtagaaac aactacaccc tggtcatcat cctgcctttc tctttatggt 3901 tacaatgata tacactgttt gagatgagga taaaatactc tgagtccaaa ccgggcccct 3961 ctgctaacca tgttcatgcc ttcttctctt tcctacagct cctgggcaac gtgctggttg 4021 ttgtgctgtc tcatcatttt ggcaaagaat tcctcgacgg atccaccggt cgccaccatg 4081 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc 4141 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc 4201 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc 4261 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag 4321 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc 4381 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg 4441 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag 4501 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc 4561 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac 4621 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac 4681 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg 4741 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc 4801 ggccgccacc gcggtggagc tcgaattaat tcatcgatga tgatccagac atgataagat 4861 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg 4921 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca 4981 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa 5041 gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcctctagat cagatccgaa 5101 gatacggcca cgggtgctct tgatcctgtg gctgattttg gactgtgctg ctcgcagctg 5161 ctgatgaatc acatacttcc tccattttct tccactgatt gactgttata atttccctaa 5221 tttccaggtc aaggtgctgt gcattgtggt aatagatgtg acatgacgtc acttccaaag 5281 gaccaatgaa catgtctgac caatttcata taatgtgaaa acgattttca taggcagaat 5341 aaataacatt taaattaaac tgggcatcag cgcaattcaa ttggtttggt aatagcaagg 5401 gaaaatagaa tgaagtgatc tccaaaaaat aagtactttt tgactgtaaa taaaattgta 5461 aggagtaaaa agtacttttt tttctaaaaa aatgtaatta agtaaaagta aaagtattga 5521 tttttaattg tactcaagta aagtaaaaat ccccaaaaat aatacttaag tacagtaatc 5581 aagtaaaatt actcaagtac tttacacctc tggttcttga ccccctacct tcagcaagcc 5641 cagcagatcc actagttcta gagcggccgc caccgcggtg gagctccagc ttttgttccc 5701 tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa 5761 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 5821 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 5881 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 5941 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 6001 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 6061 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 6121 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 6181 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 6241 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 6301 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 6361 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 6421 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 6481 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 6541 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 6601 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 6661 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 6721 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 6781 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 6841 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 6901 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 6961 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 7021 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 7081 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 7141 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 7201 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 7261 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 7321 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 7381 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 7441 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 7501 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 7561 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 7621 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 7681 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 7741 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 7801 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 7861 cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt 7921 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat 7981 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa 8041 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg 8101 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa 8161 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc 8221 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag 8281 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg 8341 cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 8401 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 8461 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga 8521 ctcactatag ggcgaattgg gtaccaaata gtaggaatta cccacctgta caagtgctga 8581 aaacttggat gaataagccc gtttgccatt ttctactgct attttaaatc ttttctgtat 8641 ttgtctgcat ttgtctttac ccttgaataa atgttttagt tgtttttttt caattatgac 8701 tgtgtttaac agacattatt acaataatat gtaatagtag tacacactat tattatgtaa 8761 tagtacttgt tgactgtatt tgagaactgg accgagtgag tgttacgtca cccattcaaa 8821 atgacttact tctggctcca acaaaatgaa gttaattcag ttgccatttt tcactgtatg 8881 gacatcgccg tgttggagct agacgttgcc aataagcaag aaagagccga gatgcgtcga 8941 gtctgagtca ccgttcctat ggcaacccct ctaaccaatc agaagtaagc ttgttggaag 9001 tccacagcct accacttgaa agcgggctgc acaaaatctg tcaaacgttt tgaacgttgg 9061 atgtgagagc acatactttt attaaggcat ctggttggtc agtttataat atcaacaact 9121 tgggctacag aaaagaaaag ttattacaga aattatgatt aacaagtaca tgttaaataa 9181 agattttaat atgaatgcca ccactggagc attcatgcca tttggagctt cttcctgttt 9241 ggatcactag aaggaggagg tcactcatta cagttctcat atacagtcgt tggttggttg 9301 gttggttggt agattgattg attgattgat tgattgattg attgattgat tgattgattg 9361 attgattgat tgattgattg attgattggt agtcaaaata agaaataatt tccacagatt 9421 cattacagaa atgattaaat gcatacataa aaaactgggg ggggggggga tacaacaaca 9481 cacttaagtc acatttgcct acgtaaagaa aagtaaagaa aatcaatagc tatattttac 9541 atctcctttt tttgctgtct tttaattagc cttgttttgc tgttatcttg attgaaactg 9601 taacttcttc acctgcttct tttctttgta ggttttgcca gcccagaaac aggcatggct 9661 gtgcaaggcc agagcaccat gcacaatgcg ctgcatgtct tcatgaacgg ctcaatgtcc 9721 tcagtccagg gctcagccaa cgaccccatc ttcctccttc accatgcttt cattgacagg 9781 taacaaacac gtcatgacat tagactgcac agtttttgac aaagttcata caatctgttg 9841 tttatagctg ctacaattag tgaagtttgt gaatgtactt ggatgagcag cgaaagatca 9901 attgagatca attgttagag tttggttgcc ctgcagagca aagaacaaaa aataatctgg 9961 tggctttact gcgtgaggtt attattggtg gaatagaaac acaaaacata attgcattta 10021 tttgtttaat tttttatctt atcttaactt tcatcttgca tatttgtttc ttacatcatt 10081 tctagcatct ttgagcgctg gctaagaact catcagcctc cccggtccat ctacccacgt 10141 accaatgcac caattggcca caatgacggc tactacatgg tgccattcct tcctctttat 10201 aggaatggag actacctcct gtccaacaat gctcttggat acgagtacgc ctacctgttg 10261 gacccaggtc attgcacaac accagaaatg ccctctgatc tgcaaaagac gtgaatatct 10321 gttcagacac ccatatccac tctgttccac acaggtcaga ggtttgtcca ggagttcttg 10381 acagaggtgt aaaaagtact caaaaatttt actcaagtga aagtacaagt acttagggaa 10441 aattttactc aattaaaagt aaaagtatct ggctagaatc ttacttgagt aaaagtaaaa 10501 aagtactcca ttaaaattgt acttgagtat taaggaagta aaagtaaaag caagaaagaa 10561 aactagagat tcttgtttaa gcttttaatc tcaaaaaaca ttaaatgaaa tgcatacaag 10621 gttttatcct gctttagaac tgtttgtatt taattatcaa actataagac agacaatcta 10681 atgccagtac acgctactca aagttgtaaa acctcagatt taacttcagt agaagctgat 10741 tctcaaaatt gttagtgtca agcctagctc ttttggggct gaaaagcaat cctgcagtgc 10801 tgaaaagcct ctcacaggca gccgatgcgg gaagaggtgt attagtcttg atagagaggc 10861 tgcaaatagc aggaaacgtg agcagagact ccctggtgtc tgaaacacag gccagatggg 10921 ccctcgagga tatc //