LOCUS       Exported                6011 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  mammalian expression of human androgen receptor amino acids 507-919 
            .
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-hARdeltaNH2-6
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Simental JA, Sar M, Lane MV, French FS, Wilson EM
  TITLE     Transcriptional activation and nuclear targeting signals of the 
            human androgen receptor.
  JOURNAL   J Biol Chem. 1991 Jan 5;266(1):510-8.
  PUBMED    1985913
REFERENCE   2  (bases 1 to 6011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    2035..2657
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2234..2253)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(2681..2701)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2686..3015
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2866..3001
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2928..2947
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(3053..3072)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3054..3072)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3086..3102)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3086..3102)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3099..3121)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3110..3126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3134..3164)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3179..3200
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3317..3334)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3488..4076)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3568..3587)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4247..5107)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4870..4889
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5108..5212)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5239..5694
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5326..5345)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5536..5557
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5820..5842
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5834..5851
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5835..5851
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agaattcaga tctggtaccc tggcggcatg gtgagcagag tgccctatcc cagtcccact
      661 tgtgtcaaaa gcgaaatggg cccctggatg gatagctact ccggacctta cggggacatg
      721 cgtttggaga ctgccaggga ccatgttttg cccattgact attactttcc accccagaag
      781 acctgcctga tctgtggaga tgaagcttct gggtgtcact atggagctct cacatgtgga
      841 agctgcaagg tcttcttcaa aagagccgct gaagggaaac agaagtacct gtgcgccagc
      901 agaaatgatt gcactattga taaattccga aggaaaaatt gtccatcttg tcgtcttcgg
      961 aaatgttatg aagcagggat gactctggga gcccggaagc tgaagaaact tggtaatctg
     1021 aaactacagg aggaaggaga ggcttccagc accaccagcc ccactgagga gacaacccag
     1081 aagctgacag tgtcacacat tgaaggctat gaatgtcagc ccatctttct gaatgtcctg
     1141 gaagccattg agccaggtgt agtgtgtgct ggacacgaca acaaccagcc cgactccttt
     1201 gcagccttgc tctctagcct caatgaactg ggagagagac agcttgtaca cgtggtcaag
     1261 tgggccaagg ccttgcctgg cttccgcaac ttacacgtgg acgaccagat ggctgtcatt
     1321 cagtactcct ggatggggct catggtgttt gccatgggct ggcgatcctt caccaatgtc
     1381 aactccagga tgctctactt cgcccctgat ctggttttca atgagtaccg catgcacaag
     1441 tcccggatgt acagccagtg tgtccgaatg aggcacctct ctcaagagtt tggatggctc
     1501 caaatcaccc cccaggaatt cctgtgcatg aaagcactgc tactcttcag cattattcca
     1561 gtggatgggc tgaaaaatca aaaattcttt gatgaacttc gaatgaacta catcaaggaa
     1621 ctcgatcgta tcattgcatg caaaagaaaa aatcccacat cctgctcaag acgcttctac
     1681 cagctcacca agctcctgga ctccgtgcag cctattgcga gagagctgca tcagttcact
     1741 tttgacctgc taatcaagtc acacatggtg agcgtggact ttccggaaat gatggcagag
     1801 atcatctctg tgcaagtgcc caagatcctt tctgggaaag tcaagcccat ctatttccac
     1861 acccagtgaa gcattggaaa ccctatttcc ccaccccagc tcatgccccc tttcagatgt
     1921 cttctgcctg ttataactct gcactactcc tctgcagtgc cttggggaat ttcctctatt
     1981 gatgtacagt ctgtcatgag cttgcatgcc tgcaggtcga ctctagagga tcccgggtgg
     2041 catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc
     2101 accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat
     2161 aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt
     2221 agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact
     2281 gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga
     2341 ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc
     2401 accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct
     2461 cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgatttta
     2521 aaataactat accagcagga ggacgtccag acacagcata ggctacctgg ccatgcccaa
     2581 ccggtgggac atttgagttg cttgcttggc actgtcctct catgcgttgg gtccactcag
     2641 tagatgcctg ttgaattggg tacgcggcca gcttggctgt ggaatgtgtg tcagttaggg
     2701 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     2761 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     2821 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     2881 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     2941 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     3001 ctaggctttt gcaaaaagct cctcgaggaa ctgaaaaacc agaaagttaa ttccctatag
     3061 tgagtcgtat taaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc
     3121 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3181 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     3241 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     3301 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     3361 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     3421 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     3481 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3541 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3601 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     3661 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     3721 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     3781 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     3841 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     3901 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     3961 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     4021 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     4081 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     4141 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     4201 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     4261 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     4321 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     4381 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     4441 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     4501 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     4561 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     4621 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     4681 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     4741 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     4801 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     4861 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     4921 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     4981 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     5041 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     5101 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     5161 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     5221 cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     5281 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     5341 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     5401 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     5461 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     5521 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     5581 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     5641 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc
     5701 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     5761 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     5821 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg atctatacat tgaatcaata
     5881 ttggcaatta gccatattag tcattggtta tatagcataa atcaatattg gctattggcc
     5941 attgcatacg ttgtatctat atcataatat gtacatttat attggctcat gtccaatatg
     6001 accgccatgt t
//
