LOCUS Exported 6492 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION mammalian expression of human androgen receptor ligand binding domain amino acid residues 624-919 with N-terminal double HA tag. ACCESSION . VERSION . KEYWORDS pcDNA3HA-AR-LBD SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6492) AUTHORS He B, Kemppainen JA, Voegel JJ, Gronemeyer H, Wilson EM TITLE Activation function 2 in the human androgen receptor ligand binding domain mediates interdomain communication with the NH(2)-terminal domain. JOURNAL J Biol Chem. 1999 Dec 24;274(52):37219-25. doi: 10.1074/jbc.274.52.37219. PUBMED 10601285 REFERENCE 2 (bases 1 to 6492) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6492 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..584 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 629..648 /label=T7 /note="T7 promoter, forward primer" promoter 629..647 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 663..689 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 693..719 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" promoter complement(1811..1829) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(1812..1829) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(1849..1866) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 1855..2079 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 2125..2553 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2212..2231) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2422..2443 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(2562..2582) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 2567..2896 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 2747..2882 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2809..2828 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2963..3757 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3017..3036) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3627..3646 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 3931..4052 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3968..3987) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4022..4041 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(4101..4117) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4101..4117) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4114..4136) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4125..4141 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4149..4179) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4194..4215 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4332..4349) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4503..5091) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4583..4602) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5262..6122) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5885..5904 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6123..6227) /gene="bla" /label=AmpR promoter primer_bind complement(6302..6321) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag 601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagcta 661 tgtacccata cgatgttcct gactatgcgg gctatcccta tgacgtcccg gactatgcaa 721 gcttggtacc gttaacatgg atccgtcgac atatgactct gggagcccgg aagctgaaga 781 aacttggtaa tctgaaacta caggaggaag gagaggcttc cagcaccacc agccccactg 841 aggagacaac ccagaagctg acagtgtcac acattgaagg ctatgaatgt cagcccatct 901 ttctgaatgt cctggaagcc attgagccag gtgtagtgtg tgctggacac gacaacaacc 961 agcccgactc ctttgcagcc ttgctctcta gcctcaatga actgggagag agacagcttg 1021 tacacgtggt caagtgggcc aaggccttgc ctggcttccg caacttacac gtggacgacc 1081 agatggctgt cattcagtac tcctggatgg ggctcatggt gtttgccatg ggctggcgat 1141 ccttcaccaa tgtcaactcc aggatgctct acttcgcccc tgatctggtt ttcaatgagt 1201 accgcatgca caagtcccgg atgtacagcc agtgtgtccg aatgaggcac ctctctcaag 1261 agtttggatg gctccaaatc accccccagg aattcctgtg catgaaagca ctgctactct 1321 tcagcattat tccagtggat gggctgaaaa atcaaaaatt ctttgatgaa cttcgaatga 1381 actacatcaa ggaactcgat cgtatcattg catgcaaaag aaaaaatccc acatcctgct 1441 caagacgctt ctaccagctc accaagctcc tggactccgt gcagcctatt gcgagagagc 1501 tgcatcagtt cacttttgac ctgctaatca agtcacacat ggtgagcgtg gactttccgg 1561 aaatgatggc agagatcatc tctgtgcaag tgcccaagat cctttctggg aaagtcaagc 1621 ccatctattt ccacacccag tgaagcattg gaaaccctat ttccccaccc cagctcatgc 1681 cccctttcag atgtcttctg cctgttataa ctctgcacta ctcctctgca gtgccttggg 1741 gaatttcctc tattgatgta cagtctgtca tgagcttgca tgcctgcagg tcgactctag 1801 agggccctat tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc 1861 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag 1921 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta 1981 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag 2041 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca 2101 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg 2161 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 2221 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 2281 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 2341 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 2401 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 2461 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa 2521 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg 2581 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 2641 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 2701 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac 2761 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 2821 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 2881 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga 2941 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc 3001 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 3061 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 3121 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac 3181 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 3241 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 3301 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 3361 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 3421 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 3481 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 3541 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 3601 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 3661 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 3721 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg 3781 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat 3841 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg 3901 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac 3961 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 4021 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc 4081 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 4141 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 4201 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 4261 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 4321 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 4381 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 4441 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 4501 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 4561 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 4621 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 4681 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 4741 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 4801 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 4861 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 4921 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 4981 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 5041 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 5101 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 5161 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 5221 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 5281 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 5341 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 5401 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 5461 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 5521 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 5581 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 5641 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 5701 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 5761 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 5821 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 5881 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 5941 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 6001 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 6061 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 6121 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 6181 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 6241 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca 6301 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg 6361 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca 6421 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca 6481 gatatacgcg tt //