LOCUS Exported 8069 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS 3120 pSFFV-neo Bcl-XL SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8069) AUTHORS Chao DT, Linette GP, Boise LH, White LS, Thompson CB, Korsmeyer SJ TITLE Bcl-XL and Bcl-2 repress a common pathway of cell death. JOURNAL J Exp Med 1995 Sep 1;182(3):821-8. PUBMED 7650488 REFERENCE 2 (bases 1 to 8069) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8069 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 98..115 /label=L4440 /note="L4440 vector, forward primer" protein_bind 232..253 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 268..298 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 306..322 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 311..333 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 330..346 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 330..346 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" promoter 367..385 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" intron 2108..2173 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 2303..2323 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 2748..2882 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2891..2911) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 2896..3225 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 3076..3211 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3138..3157 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3582..4376 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3636..3655) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4246..4265 /label=Neo-F /note="Neomycin resistance gene, forward primer" intron 4796..4861 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 4991..5011 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 5445..5579 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5576..5592) /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5576..5592) /label=pBluescriptSK /note="For pBluescript vector" primer_bind complement(5624..5643) /label=T7 /note="T7 promoter, forward primer" promoter complement(5625..5643) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(5650..5667) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(5650..5666) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5659..5681) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 5808..6263 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5895..5914) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6105..6126 /label=F1ori-F /note="F1 origin, forward primer" promoter 6289..6393 /gene="bla" /label=AmpR promoter CDS 6394..7254 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6612..6631) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 7425..8013 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7914..7933 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 61 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc 121 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 181 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 241 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg 301 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa 361 gctcgaaatt aaccctcact aaagggaaca aaagctggta ccgggccccc cctcgaggtc 421 gaccacaaca ctgctctacg ggcatgagtg gccaccttcc attttttttt taagtgtgtg 481 tgctgacgtc acaagaactc aggtactctt acttcctaca tggtacgagt tcttaccact 541 tagccatttc ttcatcctga aagaccccac caagttgctt agcctgatag ccgcagtaac 601 gccattttgc aaggcatgga aaaataccaa accaagaata gggaagttca gatcaagggc 661 gggtacacga aaacagctaa cgttgggcca aacaagatat ctgcggtaag cagtttcggc 721 cccggcccgg ggccaagaac agatggtccc cagatatggc ccaaccctca gcagtttctt 781 aagacccatc agatgtttcc aggctccccc aaggacctga aatgaccctg tgccttattt 841 gaattaacca atcagcccgc ttctcgcttc tgttcgcgcg cttttgcttc ccgagctcta 901 taaaagagct cacaacccct cactcggcgc gccagtcctc cgacagactg agtcgcccgg 961 gtacccgtgt tcccaataaa gcctcttgct gattgcatcc gaatcgtgga ctcgctgatc 1021 cttgggaggg tctcctcaga ttgattgact gcccacctcg ggggtctttc atttgggggc 1081 tcgtccggga tttggagacc cccgcccagg gaccaccgac ccaccgtcgg gaggtaagct 1141 ggccagcgat cgttttgtct ccgtctctgt ctttggaatt cttggacaat ggactggttg 1201 agcccatccc tattataaaa atgtctcaga gcaaccggga gctggtggtt gactttctct 1261 cctacaagct ttcccagaaa ggatacagct ggagtcagtt tagtgatgtg gaagagaaca 1321 ggactgaggc cccagaaggg actgaatcgg agatggagac ccccagtgcc atcaatggca 1381 acccatcctg gcacctggca gacagccccg cggtgaatgg agccactggc cacagcagca 1441 gtttggatgc ccgggaggtg atccccatgg cagcagtaaa gcaagcgctg agggaggcag 1501 gcgacgagtt tgaactgcgg taccggcggg cattcagtga cctgacatcc cagctccaca 1561 tcaccccagg gacagcatat cagagctttg aacaggtagt gaatgaactc ttccgggatg 1621 gggtaaactg gggtcgcatt gtggcctttt tctccttcgg cggggcactg tgcgtggaaa 1681 gcgtagacaa ggagatgcag gtattggtga gtcggatcgc agcttggatg gccacttacc 1741 tgaatgacca cctagagcct tggatccagg agaacggcgg ctgggatact tttgtggaac 1801 tctatgggaa caatgcagca gccgagagcc gaaagggcca ggaacgcttc aaccgctggt 1861 tcctgacggg catgactgtg gccggcgtgg ttctgctggg ctcactcttc agtcggaaat 1921 gaccagacac tgaccatcca ctctacgaat tcctgcagcc cgtgccctta aacgcctggt 1981 tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgcc ggatctttgt 2041 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag 2101 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt 2161 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt 2221 taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc 2281 tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt 2341 tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg 2401 ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa 2461 atattctgta acctttataa gtaggcataa cagttataat cataacatac tgttttttct 2521 tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt 2581 tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag 2641 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac 2701 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg 2761 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 2821 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga 2881 tcccccctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 2941 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 3001 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 3061 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 3121 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 3181 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tcacgctgcc 3241 gcaagcactc agggcgcaag ggctgctaaa ggaagcggaa cacgtagaaa gccagtccgc 3301 agaaacggtg ctgaccccgg atgaatgtca gctactgggc tatctggaca agggaaaacg 3361 caagcgcaaa gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg 3421 cggttttatg gacagcaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg 3481 ggaagccctg caaagtaaac tggatggctt tcttgccgcc aaggatctga tggcgcaggg 3541 gatcaagatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat 3601 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac 3661 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc 3721 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc 3781 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag 3841 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc 3901 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg 3961 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc 4021 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc 4081 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga 4141 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca 4201 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg 4261 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg 4321 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg 4381 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga 4441 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg 4501 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccaccccg ggctcgatcc 4561 cctcgcgagt tggttcagct gctgcctgag gctggacgac ctcgcggagt tctaccggca 4621 gtgcaaatcc gtcggcatcc aggaaaccag cagcggctat ccgcgcatcc atgcccccga 4681 actgcaggag tggggaggca cgatggccgc tttggtccgg atctttgtga aggaacctta 4741 cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa 4801 tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta 4861 gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa 4921 cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca 4981 ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt 5041 gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta 5101 caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac 5161 ctttataagt aggcataaca gttataatca taacatactg ttttttctta ctccacacag 5221 gcatagaggg catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag 5281 ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga 5341 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc 5401 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag 5461 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 5521 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc 5581 actagttcta gagcggccgc caccgcggtg gagctccaat tcgccctata gtgagtcgta 5641 ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca 5701 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg 5761 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag 5821 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 5881 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 5941 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 6001 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 6061 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 6121 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc 6181 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa 6241 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct 6301 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 6361 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc 6421 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 6481 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 6541 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 6601 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc 6661 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 6721 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 6781 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 6841 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 6901 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc 6961 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 7021 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 7081 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 7141 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 7201 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 7261 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 7321 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 7381 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 7441 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 7501 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 7561 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 7621 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 7681 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 7741 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 7801 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 7861 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 7921 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 7981 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 8041 ttcctggcct tttgctggcc ttttgctca //