LOCUS Exported 11076 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expression of human EPHB4 and GFP in mammalian cells. ACCESSION . VERSION . KEYWORDS FUW-ubiquitin-EphB4-SV40-GFP (B4G) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11076) AUTHORS Cortina C, Palomo-Ponce S, Iglesias M, Fernandez-Masip JL, Vivancos A, Whissell G, Huma M, Peiro N, Gallego L, Jonkheer S, Davy A, Lloreta J, Sancho E, Batlle E TITLE EphB-ephrin-B interactions suppress colorectal cancer progression by compartmentalizing tumor cells. JOURNAL Nat Genet. 2007 Nov;39(11):1376-83. Epub 2007 Sep 30. PUBMED 17906625 REFERENCE 2 (bases 1 to 11076) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11076 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 94..473 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 475..673 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 628..648 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 691..871 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 918..1043 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1536..1769 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1954..1998 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2296..2413 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2472..3683 /label=UbC promoter /note="human ubiquitin C promoter" primer_bind 3520..3539 /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" promoter 7633..7990 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(7656..7676) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 7841..7976 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7903..7922 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 8038..8085 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 8104..8478 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 8608..8729 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8645..8664) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8699..8718 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8778..8794) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8778..8794) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8791..8813) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8802..8818 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8826..8856) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8871..8892 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(9009..9026) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9180..9768) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9260..9279) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9939..10799) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10562..10581 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10800..10904) /gene="bla" /label=AmpR promoter primer_bind complement(10979..10998) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc 61 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat 121 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac 181 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa 241 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt 301 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc 361 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat 421 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc 481 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc 541 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa 601 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg 661 tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc 721 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 781 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 841 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga 901 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa 961 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg 1021 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa 1081 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa 1141 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta 1201 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat 1261 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca 1321 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag 1381 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta 1441 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga 1501 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg 1561 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa 1621 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag 1681 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg 1741 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt 1801 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg 1861 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac 1921 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta 1981 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa 2041 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct 2101 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc 2161 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac 2221 agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc 2281 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt taattaaggc 2461 cggcctgcag cggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac 2521 ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac 2581 gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa 2641 ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg 2701 aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg 2761 tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc 2821 gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg 2881 gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt 2941 ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt 3001 tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag 3061 gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag aacccaaggt 3121 cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct ggggcaccat 3181 ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc gtctgttgcg 3241 ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg agcgcgcgcc 3301 ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg ggccacctgc 3361 cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc ctagggtagg 3421 ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg aggcgtcagt 3481 ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg ttttgaacta 3541 tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt gaaatgtaat 3601 catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc 3661 cgtttttggc ttttttgtta gacaggatcc tacccgagtg aggcggcgcc atggagctcc 3721 gggtgctgct ctgctgggct tcgttggccg cagctttgga agagaccctg ctgaacacaa 3781 aattggaaac tgctgatctg aagtgggtga cattccctca ggtggacggg cagtgggagg 3841 aactgagcgg cctggatgag gaacagcaca gcgtgcgcac ctacgaagtg tgtgacgtgc 3901 agcgtgcccc gggccaggcc cactggcttc gcacaggttg ggtcccacgg cggggcgccg 3961 tccacgtgta cgccacgctg cgcttcacca tgctcgagtg cctgtccctg cctcgggctg 4021 ggcgctcctg caaggagacc ttcaccgtct tctactatga gagcgatgcg gacacggcca 4081 cggccctcac gccagcctgg atggagaacc cctacatcaa ggtggacacg gtggccgcgg 4141 agcatctcac ccggaagcgc cctggggccg aggccaccgg gaaggtgaat gtcaggacgc 4201 tgcgtctggg accgctcagc aaggctggct tctacctggc cttccaggac cagggtgcct 4261 gcatggccct gctatccctg cacctcttct acaaaaagtg cgcccagctg actgtgaacc 4321 tgactcgatt cccggagact gtgcctcggg agctggttgt gcccgtggcc ggtagctgcg 4381 tggtggatgc cgtccccgcc cctggcccca gccccagcct ctactgccgt gaggatggcc 4441 agtgggccga acagccggtc acgggctgca gctgtgctcc ggggttcgag gcagctgagg 4501 ggaacaccaa gtgccgagcc tgtgcccagg gcaccttcaa gcccctgtca ggagaagggt 4561 cctgccagcc atgcccagcc aatagccact ctaacaccat tggatcagcc gtctgccagt 4621 gccgcgtcgg gtacttccgg gcacgcacag acccccgggg tgcaccctgc accacccctc 4681 cttcggctcc gcggagcgtg gtttcccgcc tgaacggctc ctccctgcac ctggaatgga 4741 gtgcccccct ggagtctggt ggccgagagg acctcaccta cgccctccgc tgccgggagt 4801 gccgacccgg aggctcctgt gcgccctgcg ggggagacct gacttttgac cccggccccc 4861 gggacctggt ggagccctgg gtggtggttc gagggctacg tcctgacttc acctatacct 4921 ttgaggtcac tgcattgaac ggggtatcct ccttagccac ggggcccgtc ccatttgagc 4981 ctgtcaatgt caccactgac cgagaggtac ctcctgcagt gtccgacatc cgggtgacgc 5041 ggtcctcacc cagcagcttg agcctggcct gggctgttcc ccgggcaccc agtggggctg 5101 tgctggacta cgaggtcaaa taccatgaga agggcgccga gggtcccagc agcgtgcggt 5161 tcctgaagac gtcagaaaac cgggcagagc tgcgggggct gaagcgggga gccagctacc 5221 tggtgcaggt acgggcgcgc tctgaggccg gctacgggcc cttcggccag gaacatcaca 5281 gccagaccca actggatgag agcgagggct ggcgggagca gctggccctg attgcgggca 5341 cggcagtcgt gggtgtggtc ctggtcctgg tggtcattgt ggtcgcagtt ctctgcctca 5401 ggaagcagag caatgggaga gaagcagaat attcggacaa acacggacag tatctcatcg 5461 ggcatggtac taaggtctac atcgacccct tcacttatga agaccctaat gaggctgtga 5521 gggaatttgc aaaagagatc gatgtctcct acgtcaagat tgaagaggtg attggtgcag 5581 gtgagtttgg cgaggtgtgc cgggggcggc tcaaggcccc agggaagaag gagagctgtg 5641 tggcaatcaa gaccctgaag ggtggctaca cggagcggca gcggcgtgag tttctgagcg 5701 aggcctccat catgggccag ttcgagcacc ccaatatcat ccgcctggag ggcgtggtca 5761 ccaacagcat gcccgtcatg attctcacag agttcatgga gaacggcgcc ctggactcct 5821 tcctgcggct aaacgacgga cagttcacag tcatccagct cgtgggcatg ctgcggggca 5881 tcgcctcggg catgcggtac cttgccgaga tgagctacgt ccaccgagac ctggctgctc 5941 gcaacatcct agtcaacagc aacctcgtct gcaaagtgtc tgactttggc ctttcccgat 6001 tcctggagga gaactcttcc gatcccacct acacgagctc cctgggagga aagattccca 6061 tccgatggac tgccccggag gccattgcct tccggaagtt cacttccgcc agtgatgcct 6121 ggagttacgg gattgtgatg tgggaggtga tgtcatttgg ggagaggccg tactgggaca 6181 tgagcaatca ggacgtgatc aatgccattg aacaggacta ccggctgccc ccgcccccag 6241 actgtcccac ctccctccac cagctcatgc tggactgttg gcagaaagac cggaatgccc 6301 ggccccgctt cccccaggtg gtcagcgccc tggacaagat gatccggaac cccgccagcc 6361 tcaaaatcgt ggcccgggag aatggcgggg cctcacaccc tctcctggac cagcggcagc 6421 ctcactactc agcttttggc tctgtgggcg agtggcttcg ggccatcaaa atgggaagat 6481 acgaagaaag tttcgcagcc gctggctttg gctccttcga gctggtcagc cagatctctg 6541 ctgaggacct gctccgaatc ggagtcactc tggcgggaca ccagaagaaa atcttggcca 6601 gtgtccagca catgaagtcc caggccaagc cgggaacccc gggtgggaca ggaggaccgg 6661 ccccgcagta ctgacctgca ggaactcccc accccaggga caccgcctcc ccattttccg 6721 gggcagagtg gggactcaca gaggccccca gccctgtgcc ccgctggatt gcactttgag 6781 cccgtggggt gaggagttgg caatttggag agacaggatt tgggggttct gccataatag 6841 gaggggaaaa tcacccccca gccacctcgg ggaactccag accaagggtg agggcgcctt 6901 tccctcagga ctgggtgtga ccagaggaaa aggaagtgcc caacatctcc cagcctcccc 6961 aggtgccccc ctcaccttga tgggtgcgtt cccgcagacc aaagagagtg tgactccctt 7021 gccagctcca gagtgggggg gctgtcccag ggggcaagaa ggggtgtcag ggcccagtga 7081 caaaatcatt ggggtttgta gtcccaactt gctgctgtca ccaccaaact caatcatttt 7141 tttcccttgt aaatgcccct cccccagctg ctgccttcat attgaaggtt tttgagtttt 7201 gtttttggtc ttaatttttc tccccgttcc ctttttgttt cttcgttttg tttttctacc 7261 gtccttgtca taactttgtg ttggagggaa cctgtttcac tatggcctcc tttgcccaag 7321 ttgaaacagg ggcccatcat catgtctgtt tccagaacag tgccttggtc atcccacatc 7381 cccggacccc gcctgggacc cccaagctgt gtcctatgaa ggggtgtggg gtgaggtagt 7441 gaaaagggcg gtagttggtg gtggaaccca gaaacggacg ccggtgcttg gaggggttct 7501 taaattatat ttaaaaaagt aactttttgt ataaataaaa gaaaatggga cgtctcccag 7561 ctccaggggt aaccggtgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg 7621 gttaggtacc ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg 7681 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 7741 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 7801 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc 7861 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 7921 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 7981 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt 8041 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa 8101 accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg 8161 gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc 8221 ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg 8281 gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg 8341 gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag 8401 cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg 8461 gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa 8521 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 8581 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa 8641 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 8701 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag 8761 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 8821 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 8881 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 8941 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 9001 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 9061 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 9121 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 9181 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 9241 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 9301 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 9361 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 9421 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 9481 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 9541 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 9601 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 9661 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 9721 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 9781 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 9841 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 9901 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 9961 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 10021 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 10081 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 10141 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 10201 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 10261 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 10321 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 10381 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 10441 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 10501 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 10561 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 10621 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 10681 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 10741 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 10801 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 10861 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 10921 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta 10981 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg 11041 tcgctgagta gtgcgcgagc aaaatttaag ctacaa //