LOCUS       Exported                8794 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Allows conditional recruitment of AMPA receptors to the postsynaptic
            membrane.
ACCESSION   .
VERSION     .
KEYWORDS    mCh-GluA1-CIB
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8794)
  AUTHORS   Sinnen BL, Bowen AB, Forte JS, Hiester BG, Crosby KC, Gibson ES, 
            Dell'Acqua ML, Kennedy MJ
  TITLE     Optogenetic Control of Synaptic Composition and Function.
  JOURNAL   Neuron. 2017 Feb 8;93(3):646-660.e5. doi: 
            10.1016/j.neuron.2016.12.037. Epub 2017 Jan 26.
  PUBMED    28132827
REFERENCE   2  (bases 1 to 8794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8794
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        19..1307
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     CDS             1463..2167
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(1605..1623)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1873..1892
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2168..2185
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     polyA_signal    5588..5709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5625..5644)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5679..5698
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5716..6171)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5853..5874)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6065..6084
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6198..6302
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6304..6661
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(6327..6347)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6512..6647
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6574..6593
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6696..7490
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6750..6769)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7360..7379
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7678..7697)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7722..7769
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8098..8686
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8587..8606
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgcattagt tattataaca ttatggcctt aggtcacttc atctccatgg ggttcttctt
       61 ctgattttct agaaaatgag atgggggtgc agagagcttc ctcagtgacc tgcccagggt
      121 cacatcagaa atgtcagagc tagaacttga actcagatta ctaatcttaa attccatgcc
      181 ttgggggcat gcaagtacga tatacagaag gagtgaactc attagggcag atgaccaatg
      241 agtttaggaa agaagagtcc agggcagggt acatctacac cacccgccca gccctgggtg
      301 agtccagcca cgttcacctc attatagttg cctctctcca gtcctacctt gacgggaagc
      361 acaagcagaa actgggacag gagccccagg agaccaaatc ttcatggtcc ctctgggagg
      421 atgggtgggg agagctgtgg cagaggcctc aggaggggcc ctgctgctca gtggtgacag
      481 ataggggtga gaaagcagac agagtcattc cgtcagcatt ctgggtctgt ttggtacttc
      541 ttctcacgct aaggtggcgg tgtgatatgc acaatggcta aaaagcaggg agagctggaa
      601 agaaacaagg acagagacag aggccaagtc aaccagacca attcccagag gaagcaaaga
      661 aaccattaca gagactacaa gggggaaggg aaggagagat gaattagctt cccctgtaaa
      721 ccttagaacc cagctgttgc cagggcaacg gggcaatacc tgtctcttca gaggagatga
      781 agttgccagg gtaactacat cctgtctttc tcaaggacca tcccagaatg tggcacccac
      841 tagccgttac catagcaact gcctctttgc cccacttaat cccatcccgt ctgttaaaag
      901 ggccctatag ttggaggtgg gggaggtagg aagagcgatg atcacttgtg gactaagttt
      961 gttcgcatcc ccttctccaa ccccctcagt acatcaccct gggggaacag ggtccacttg
     1021 ctcctgggcc cacacagtcc tgcagtattg tgtatataag gccagggcaa agaggagcag
     1081 gttttaaagt gaaaggcagg caggtgttgg ggaggcagtt accggggcaa cgggaacagg
     1141 gcgtttcgga ggtggttgcc atggggacct ggatgctgac gaaggctcgc gaggctgtga
     1201 gcagccacag tgccctgctc agaagcccca agctcgtcag tcaagccggt tctccgtttg
     1261 cactcaggag cacgggcagg cgagtggccc ctagttctgg gggcagcggg ggatccccgg
     1321 gtaccgggta caagtccgga ctcagatctc gagctccacc atgccgtaca tctttgcctt
     1381 tttctgcacc ggttttctag gtgcggttgt gggtgccaat gcgcgaggtg gaggcggtgg
     1441 gggagggggt ggaggaacgc gtgtgagcaa gggcgaggag gataacatgg ccatcatcaa
     1501 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
     1561 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     1621 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     1681 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     1741 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     1801 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     1861 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     1921 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     1981 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     2041 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     2101 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     2161 gtacaagctg gttccgcgtg gatccacgcg tggaggtgga gggggcggag gtggcggggg
     2221 ttcgcgcttc cccaacaata tccagatagg ggggttattt ccaaaccaac aatcacagga
     2281 acatgcggct tttaggtttg ctttgtcaca actcacggag ccccccaagc tgcttcccca
     2341 gatcgatatt gtgaacatca gcgacagctt tgagatgact taccgtttct gttcccagtt
     2401 ctccaaagga gtctatgcca tctttggatt ttatgaacga aggactgtca acatgctgac
     2461 ctccttctgt ggggccctcc atgtgtgctt cattactcca agttttcctg ttgacacatc
     2521 caatcaattt gtccttcagc tacgcccgga actacaggaa gctctcatta gcattatcga
     2581 ccattacaag tggcaaacct ttgtctacat ttatgatgct gaccggggcc tgtcagtcct
     2641 gcagagagtc ttggatacag ccgcagagaa gaactggcag gtaacggctg tcaacattct
     2701 gacaaccacc gaggaaggat accggatgct ctttcaggac ctggagaaga aaaaggagag
     2761 gctggtggtg gttgactgtg aatcagaacg cctcaacgcc atcctgggcc agatcgtgaa
     2821 gctagaaaag aatggcatcg ggtaccacta catcctcgcc aatctgggct tcatggacat
     2881 tgacttaaat aagttcaagg agagcggagc caatgtgaca ggtttccagc tggtgaacta
     2941 cacagacacg atcccagcca gaatcatgca gcaatggagg acaagtgact cccgagacca
     3001 taccagggtg gactggaaga ggccaaagta cacttctgct ctcacctatg atggtgtcaa
     3061 ggtgatggct gaggccttcc aaagcctgcg gaggcagagg attgacatat cccgccgggg
     3121 gaatgctggg gactgtctgg ctaacccagc tgtgccctgg ggtcaaggga tcgacatcca
     3181 gagagccctg cagcaggtgc gcttcgaagg tttgacagga aatgtgcagt tcaacgagaa
     3241 agggcgccgg accaattaca ccctccacgt gatcgaaatg aaacatgatg gaatccgaaa
     3301 gattggttac tggaatgaag acgataaatt tgtccccgca gccaccgacg ctcaggctgg
     3361 aggggacaac tcaagcgtcc agaataggac ctacatcgtc actactatcc tcgaagatcc
     3421 ttacgtgatg cttaaaaaga atgccaacca gtttgagggc aatgaccgct atgagggcta
     3481 ctgtgtggag ctggctgcag agatcgccaa gcacgtgggc tactcctacc gacttgagat
     3541 tgtcagcgac ggcaaatatg gagcccggga tcccgacaca aaggcttgga atggcatggt
     3601 gggagaactg gtctatggaa gagcagacgt ggctgtggct cccttgacca taaccttggt
     3661 ccgggaggaa gtcatcgact tctccaagcc attcatgagt ttgggaatct ccattatgat
     3721 taagaagcca cagaagtcca agccaggtgt cttctccttt cttgaccctt tggcctatga
     3781 gatctggatg tgtatagtgt ttgcctacat tggagtgagc gtcgtcctct tcctggtcag
     3841 ccgtttcagc ccctacgaat ggcacagcga agagtttgaa gagggacgag accagacaac
     3901 cagtgaccag tcaaatgagt ttggcatatt caacagcctg tggttctccc tgggggcctt
     3961 catgcagcaa ggatgtgaca tttcccccag gtccctgtcc ggacgcatcg tcggcggcgt
     4021 ctggtggttc ttcactttga tcatcatctc ctcgtacaca gccaacctgg ctgccttcct
     4081 gactgtggag aggatggtgt ctcccattga gagtgcagag gacctggcaa agcagacgga
     4141 aattgcttat gggacattgg aagcaggctc cactaaggag ttcttcagga gatctaaaat
     4201 cgctgtgttt gagaagatgt ggacatacat gaagtctgca gaaccatccg tgtttgttcg
     4261 gaccacagag gaaggcatga tcagagtgag aaaatctaaa ggcaaatacg cctacctcct
     4321 ggagtccacc atgaatgagt atattgagca acgaaagccc tgtgacacca tgaaagtggg
     4381 aggtaacttg gattccaaag gctatggcat tgcgacaccc aaggggtccg ccctgagaaa
     4441 tccagtaaac ctggcagtgt taaaactgaa cgagcagggg cttttggaca aattgaaaaa
     4501 caaatggtgg tacgacaagg gcgagtgcgg cagcggggga ggtgactcca aggacaagac
     4561 cagcgctttg agcctcagca atgtggcagg cgtgttctac atcctgattg gagggctggg
     4621 actggccatg ctggttgcct taatcgagtt ctgctacaaa tcccgtagcg agtcgaagcg
     4681 gatgaagggt ttctgtttga tcccacagca atccatcaat gaagccatac ggacatcgac
     4741 cctcccccgg aacagtgggg caggagccag cggaggaggc ggcagtggag agaatggccg
     4801 ggtggtcagc caggacttcc ccaagtccat gcaatccatt ccctgcatga gtcacagttc
     4861 agggatgccc ttgggagcca caggattggg gaattctgca gtcgacggta ccgcggatag
     4921 tgctggtagt gctggtagtg ctggtaatgg agctatagga ggtgaccttt tgctcaattt
     4981 tcctgacatg tcggtcctag agcgccaaag ggctcacctc aagtacctca atcccacctt
     5041 tgattctcct ctcgccggct tctttgccga ttcttcaatg attaccggcg gcgagatgga
     5101 cagctatctt tcgactgccg gtttgaatct tccgatgatg tacggtgaga cgacggtgga
     5161 aggtgattca agactctcaa tttcgccgga aacgacgctt gggactggaa atttcaaggc
     5221 agcgaagttt gatacagaga ctaaggattg taatgaggcg gcgaagaaga tgacgatgaa
     5281 cagagatgac ctagtagaag aaggagaaga agagaagtcg aaaataacag agcaaaacaa
     5341 tgggagcaca aaaagcatca agaagatgaa acacaaagcc aagaaagaag agaacaattt
     5401 ctctaatgat tcatctaaag tgacgaagga attggagaaa acggattata ttcattaagg
     5461 atccaccgga tctagataac tgatcataat cagccatacc acatttgtag aggttttact
     5521 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     5581 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     5641 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5701 tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     5761 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     5821 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     5881 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     5941 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     6001 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     6061 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     6121 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
     6181 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6241 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6301 gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     6361 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     6421 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     6481 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     6541 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     6601 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     6661 agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     6721 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     6781 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     6841 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
     6901 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     6961 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     7021 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     7081 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     7141 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     7201 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
     7261 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     7321 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     7381 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     7441 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     7501 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     7561 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     7621 cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga
     7681 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     7741 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     7801 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
     7861 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
     7921 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt
     7981 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8041 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8101 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8161 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8221 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     8281 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8341 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8401 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8461 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8521 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8581 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8641 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8701 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8761 atcccctgat tctgtggata accgtattac cgcc
//
