LOCUS Exported 11518 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Plant expression vector (2x35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor. ACCESSION . VERSION . KEYWORDS pMDC123SB-AtMIR390a-B/c SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11518) AUTHORS Carbonell A, Takeda A, Fahlgren N, Johnson SC, Cuperus JT, Carrington JC TITLE New Generation of Artificial MicroRNA and Synthetic Trans-Acting Small Interfering RNA Vectors for Efficient Gene Silencing in Arabidopsis. JOURNAL Plant Physiol. 2014 Mar 19. PUBMED 24647477 REFERENCE 2 (bases 1 to 11518) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11518 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 612..1163 /codon_start=1 /gene="Streptomyces hygroscopicus bar" /product="phosphinothricin acetyltransferase" /label=BlpR /note="confers resistance to bialophos or phosphinothricin" /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF WQLDFSLPVPPRPVLPVTEI" polyA_signal 1170..1344 /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" misc_feature 1422..1446 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" CDS 1871..2665 /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" rep_origin 2752..3340 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3241..3260 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3494..3511 /label=L4440 /note="L4440 vector, forward primer" misc_feature 3526..3666 /label=bom /note="basis of mobility region from pBR322" primer_bind complement(3592..3611) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 3752..3774 /label=pGEX 3' /note="pGEX vectors, reverse primer" rep_origin 4010..4204 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" CDS complement(4270..5343) /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" CDS complement(5772..6401) /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" misc_feature 7702..7726 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" primer_bind 7914..7936 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 7928..7945 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 7929..7945 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 8371..8715 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" protein_bind 8775..8799 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 9093..9123 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 9177..9836 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(9243..9262) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 10178..10483 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 10362..10381 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(10747..10771) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" terminator 10835..11087 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" primer_bind complement(11104..11120) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(11104..11120) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(11117..11139) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 11128..11144 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(11152..11182) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11197..11218 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 11409..567 /label=CaMV 35S promoter (enhanced) /note="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" ORIGIN 1 gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca 61 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt 121 caaagcaagt ggattgatgt gaacatggtg gagcacgaca ctctcgtcta ctccaagaat 181 atcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata 241 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta 301 gaaaaggaag gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa 361 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa 421 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac 481 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt 541 tcatttcatt tggagaggac acgctgaaat caccagtctc tctctacaaa tctatctctc 601 tcgagtctac catgagccca gaacgacgcc cggccgacat ccgccgtgcc accgaggcgg 661 acatgccggc ggtctgcacc atcgtcaacc actacatcga gacaagcacg gtcaacttcc 721 gtaccgagcc gcaggaaccg caggagtgga cggacgacct cgtccgtctg cgggagcgct 781 atccctggct cgtcgccgag gtggacggcg aggtcgccgg catcgcctac gcgggcccct 841 ggaaggcacg caacgcctac gactggacgg ccgagtcgac cgtgtacgtc tccccccgcc 901 accagcggac gggactgggc tccacgctct acacccacct gctgaagtcc ctggaggcac 961 agggcttcaa gagcgtggtc gctgtcatcg ggctgcccaa cgacccgagc gtgcgcatgc 1021 acgaggcgct cggatatgcc ccccgcggca tgctgcgggc ggccggcttc aagcacggga 1081 actggcatga cgtgggtttc tggcagctgg acttcagcct gccggtaccg ccccgtccgg 1141 tcctgcccgt caccgagatt tgactcgagt ttctccataa taatgtgtga gtagttccca 1201 gataagggaa ttagggttcc tatagggttt cgctcatgtg ttgagcatat aagaaaccct 1261 tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca 1321 aaatccagta ctaaaatcca gatcccccga attaattcgg cgttaattca gtacattaaa 1381 aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc 1441 ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat 1501 acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact 1561 ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac 1621 ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg 1681 atcaccgcgg tttcaaaatc ggctccgtcg atactatgtt atacgccaac tttgaaaaca 1741 actttgaaaa agctgttttc tggtatttaa ggttttagaa tgcaaggaac agtgaattgg 1801 agttcgtctt gttataatta gcttcttggg gtatctttaa atactgtaga aaagaggaag 1861 gaaataataa atggctaaaa tgagaatatc accggaattg aaaaaactga tcgaaaaata 1921 ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag gtatataagc tggtgggaga 1981 aaatgaaaac ctatatttaa aaatgacgga cagccggtat aaagggacca cctatgatgt 2041 ggaacgggaa aaggacatga tgctatggct ggaaggaaag ctgcctgttc caaaggtcct 2101 gcactttgaa cggcatgatg gctggagcaa tctgctcatg agtgaggccg atggcgtcct 2161 ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag attatcgagc tgtatgcgga 2221 gtgcatcagg ctctttcact ccatcgacat atcggattgt ccctatacga atagcttaga 2281 cagccgctta gccgaattgg attacttact gaataacgat ctggccgatg tggattgcga 2341 aaactgggaa gaagacactc catttaaaga tccgcgcgag ctgtatgatt ttttaaagac 2401 ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac ctgggagaca gcaacatctt 2461 tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg agaagcggca gggcggacaa 2521 gtggtatgac attgccttct gcgtccggtc gatcagggag gatatcgggg aagaacagta 2581 tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa taaaatatta 2641 tattttactg gatgaattgt tttagtacct agaatgcatg accaaaatcc cttaacgtga 2701 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 2761 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 2821 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 2881 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc 2941 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 3001 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 3061 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 3121 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 3181 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 3241 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 3301 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 3361 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc 3421 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg 3481 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt 3541 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg 3601 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg 3661 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg 3721 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca 3781 ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg 3841 cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag ccatttttga 3901 gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc gcagcgaccg 3961 aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc 4021 caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa aatcgccttt 4081 tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg gctttgggtt 4141 cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt acgtgctatc 4201 cacaggaaag agaacttttc gacctttttc ccctgctagg gcaatttgcc ctagcatctg 4261 ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt agcgcatgac 4321 taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca ggtcatttga 4381 cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc tgccactgcg 4441 ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc ggcgtgccag 4501 gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt gaaccgtcag 4561 cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta gctcgatctc 4621 gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa tcaaggcttc 4681 accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct gcatggcaac 4741 gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct ttccgccatc 4801 ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt 4861 gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa ccgccatcag 4921 taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa cctctacgtg 4981 cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct tcgacagacg 5041 gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat agagcatcgg 5101 aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc 5161 tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc acgattcacc 5221 ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc 5281 caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct 5341 cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca ggcaacccag 5401 ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac ggcgtcggtg 5461 cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca tctactcatt 5521 tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca gctcggtaat 5581 ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg ccggcaactg 5641 aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg cagccttgct 5701 gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca ttttctcttt 5761 acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg gacctcgcgg 5821 gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc agtgcctggg 5881 tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc ggccagcgcc 5941 tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct 6001 tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg 6061 gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc tgccttgatc 6121 gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg 6181 ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt 6241 tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga atcgactaac 6301 agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg 6361 cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc ttgcgtattt 6421 gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagctg ttttactcaa 6481 atacacatca cctttttaga cggcggcgct cggtttcttc agcggccaag ctggccggcc 6541 aggccgccag cttggcatca gacaaaccgg ccaggatttc atgcagccgc acggttgaga 6601 cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg 6661 taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt cctcttggcg 6721 ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc 6781 gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt 6841 cgagcgatgc acgccaagca gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat 6901 cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc caaacttcac 6961 gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc 7021 gaactcggca atgccggcga acacggtcaa caccatgcgg ccggccggcg tggtggtgtc 7081 ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat 7141 gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg tcacaacgtc 7201 gccagggcgt aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc tggtgccggt 7261 gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc gttggttggt 7321 caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg cggcgctctt 7381 gttcatggcg taatgtctcc ggttctagtc gcaagtattc tactttatgc gactaaaaca 7441 cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt 7501 gtgcgacatg tcgttttcag aagacggctg cactgaacgt cagaagccga ctgcactata 7561 gcagcggagg ggttggatca aagtactttg atcccgaggg gaaccctgtg gttggcatgc 7621 acatacaaat ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc gattattcta 7681 ataaacgctc ttttctctta ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt 7741 aaactgaagg cgggaaacga caatctgatc caagctcaag ctgctctagc attcgccatt 7801 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 7861 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc 7921 acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtca acatggtgga 7981 gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag accaaagggc 8041 aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc attgcccagc 8101 tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca aatgccatca 8161 ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg 8221 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca 8281 agtggattga tgtgataaca tggtggagca cgacacactt gtctactcca aaaatatcaa 8341 agatacagtc tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg 8401 aaacctcctc ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa 8461 ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc 8521 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga 8581 agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag 8641 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt 8701 tcatttggag aggacctcga ctctagagga tccccgggta ccgggccccc cctcgaggcg 8761 cgccaagcta tcaaacaagt ttgtacaaaa aagcaggctc cgcggccgcc cccttcacct 8821 atagggggga aaaaaaggta gtcatcagat atatattttg gtaagaaaat atagaaatga 8881 ataatttcac gtttaacgaa gaggagatga cgtgtgttcc ttcgaacccg agttttgttc 8941 gtctataaat agcaccttct cttctccttc ttcctcactt ccatcttttt agcttcacta 9001 tctctctata atcggtttta tctttctcta agtcacaacc caaaaaaaca aagtagagaa 9061 gaatctgtaa gagaccatta ggcaccccag gctttacact ttatgcttcc ggctcgtata 9121 atgtgtggat tttgagttag gagccgtcga gattttcagg agctaaggaa gctaaaatgg 9181 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt 9241 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta 9301 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca 9361 ttcttgcccg cctgatgaat gctcatccgg agttccgtat ggcaatgaaa gacggtgagc 9421 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt 9481 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc 9541 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata 9601 tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca 9661 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca 9721 aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg 9781 gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaaacgc 9841 gtggagccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg 9901 gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt atgctatgaa 9961 gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat 10021 gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc 10081 gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg 10141 aacggctctt ttgctgacga gaacaggggc tggtgaaatg cagtttaagg tttacaccta 10201 taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc 10261 cggccgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg 10321 tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat 10381 ggccagtgtg ccggtttccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa 10441 tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat 10501 acacagccag tctgcacctc gacggtctca cattggctct tcttactaca atgaaaaagg 10561 ccgaggcaaa acgcctaaaa tcacttgaga atcaattctt tttactgtcc atttaagcta 10621 tcttttataa acgtgtctta ttttctatct cttttgttta aactaagaaa ctatagtatt 10681 ttgtctaaaa caaaacatga aagaacagat tagatctcat ctttagtctc aagggtgggc 10741 gcgccgaccc agctttcttg tacaaagtgg ttcgataatt cttaattaac tagttctaga 10801 gcggccgcca ccgcggtgga gctcgaattt ccccgatcgt tcaaacattt ggcaataaag 10861 tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa 10921 ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt 10981 tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc 11041 aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg aattcgtaat 11101 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 11161 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 11221 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 11281 aatcggccaa cgcgcgggga gaggcggttt gcgtattggc tagagcagct tgccaacatg 11341 gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccaa 11401 agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc 11461 ccagctatct gtcacttcat caaaaggaca gtagaaaagg aaggtggcac ctacaaat //