LOCUS Exported 10375 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses HF-BE3-NLS with an N-terminal His Tag (His6) for bacterial expression. ACCESSION . VERSION . KEYWORDS pET42b-HF-BE3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10375) AUTHORS Rees HA, Komor AC, Yeh WH, Caetano-Lopes J, Warman M, Edge ASB, Liu DR TITLE Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery. JOURNAL Nat Commun. 2017 Jun 6;8:15790. doi: 10.1038/ncomms15790. PUBMED 28585549 REFERENCE 2 (bases 1 to 10375) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10375 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 21..38 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 39..722 /codon_start=1 /product="cytidine deaminase (C to U editing enzyme) from rat" /label=APOBEC-1 /note="can use ssDNA as a substrate (Komor et al., 2016)" /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL WATGLK" CDS 4884..5132 /codon_start=1 /product="uracil-DNA glycosylase inhibitor from a Bacillus subtilis bacteriophage (Mol et al., 1995)" /label=UGI /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML" CDS 5145..5165 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" primer_bind complement(5231..5249) /label=T7 Term /note="T7 terminator, reverse primer" terminator 5245..5292 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" rep_origin 5329..5784 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5416..5435) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5626..5647 /label=F1ori-F /note="F1 origin, forward primer" CDS complement(5876..6691) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 6599..6618 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" primer_bind 6683..6702 /label=pENTR-R /note="pENTR vectors, reverse primer" rep_origin 6813..7401 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7302..7321 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 7555..7572 /label=L4440 /note="L4440 vector, forward primer" misc_feature 7587..7726 /label=bom /note="basis of mobility region from pBR322" primer_bind complement(7652..7671) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 7812..7834 /label=pGEX 3' /note="pGEX vectors, reverse primer" CDS complement(7828..8019) /codon_start=1 /gene="rop" /product="Rop protein, which maintains plasmids at low copy number" /label=rop /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA YELYRSCLARFGDDGENL" CDS complement(8828..9910) /codon_start=1 /gene="lacI" /product="lac repressor" /label=lacI /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNHASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQLLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESWQ" primer_bind 9872..9891 /label=LacI-R /note="LacI, reverse primer" promoter complement(9911..9988) /gene="lacI" /label=lacI promoter primer_bind 10194..10213 /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind 10297..10316 /label=T7 /note="T7 promoter, forward primer" promoter 10297..10315 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 10316..10340 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 10355..2 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" ORIGIN 1 gatataccat gggcagcagt catcatcatc accatcactc ttctgaaacc ggtccggttg 61 cggttgaccc gaccctgcgt cgtcgtatcg aaccgcacga attcgaagtt ttcttcgacc 121 cgcgtgaact gcgtaaagaa acctgcctgc tgtacgaaat caactggggt ggtcgtcact 181 ctatctggcg tcacacctct cagaacacca acaaacacgt tgaagttaac ttcatcgaaa 241 aattcaccac cgaacgttac ttctgcccga acacccgttg ctctatcacc tggttcctgt 301 cttggtctcc gtgcggtgaa tgctctcgtg cgatcaccga attcctgtct cgttacccgc 361 acgttaccct gttcatctac atcgcgcgtc tgtaccacca cgcggacccg cgtaaccgtc 421 agggtctgcg tgacctgatc tcttctggtg ttaccatcca gatcatgacc gaacaggaat 481 ctggttactg ctggcgtaac ttcgttaact actctccgtc taacgaagcg cactggccgc 541 gttacccgca cctgtgggtt cgtctgtacg ttctggaact gtactgcatc atcctgggtc 601 tgccgccgtg cctgaacatc ctgcgtcgta aacagccgca gctgaccttc ttcaccatcg 661 cgctgcagtc ttgccactac cagcgtctgc cgccgcacat cctgtgggcg accggtctga 721 aatccggtag cgaaacaccg gggacttcag aatcggccac cccggagtct gataagaaat 781 actcaatagg cttagctatc ggcacaaata gcgtcggatg ggcggtgatc actgatgaat 841 ataaggttcc gtctaaaaag ttcaaggttc tgggaaatac agaccgccac agtatcaaaa 901 aaaatcttat aggggctctt ttatttgaca gtggagagac agcggaagcg actcgtctca 961 aacggacagc tcgtagaagg tatacacgtc ggaagaatcg tatttgttat ctacaggaga 1021 ttttttcaaa tgagatggcg aaagtagatg atagtttctt tcatcgactt gaagagtctt 1081 ttttggtgga agaagacaag aagcatgaac gtcatcctat ttttggaaat atagtagatg 1141 aagttgctta tcatgagaaa tatccaacta tctatcatct gcgaaaaaaa ttggtagatt 1201 ctactgataa agcggatttg cgcttaatct atttggcctt agcgcatatg attaagtttc 1261 gtggtcattt tttgattgag ggagatttaa atcctgataa tagtgatgtg gacaaactat 1321 ttatccagtt ggtacaaacc tacaatcaat tatttgaaga aaaccctatt aacgcaagtg 1381 gagtagatgc taaagcgatt ctttctgcac gattgagtaa atcaagacga ttagaaaatc 1441 tcattgctca gctccccggt gagaagaaaa atggcttatt tgggaatctc attgctttgt 1501 cattgggttt gacccctaat tttaaatcaa attttgattt ggcagaagat gctaaattac 1561 agctttcaaa agatacttac gatgatgatt tagataattt attggcgcaa attggagatc 1621 aatatgctga tttgtttttg gcagctaaga atttatcaga tgctatttta ctttcagata 1681 tcctaagagt aaatactgaa ataactaagg ctcccctatc agcttcaatg attaaacgct 1741 acgatgaaca tcatcaagac ttgactcttt taaaagcttt agttcgacaa caacttccag 1801 aaaagtataa agaaatcttt tttgatcaat caaaaaacgg atatgcaggt tatattgatg 1861 ggggagctag ccaagaagaa ttttataaat ttatcaaacc aattttagaa aaaatggatg 1921 gtactgagga attattggtg aaactaaatc gtgaagattt gctgcgcaag caacggacct 1981 ttgacaacgg ctctattccc catcaaattc acttgggtga gctgcatgct attttgagaa 2041 gacaagaaga cttttatcca tttttaaaag acaatcgtga gaagattgaa aaaatcttga 2101 cttttcgaat tccttattat gttggtccat tggcgcgtgg caatagtcgt tttgcatgga 2161 tgactcggaa gtctgaagaa acaattaccc catggaattt tgaagaagtt gtcgataaag 2221 gtgcttcagc tcaatcattt attgaacgca tgacagcctt tgataaaaat cttccaaatg 2281 aaaaagtact accaaaacat agtttgcttt atgagtattt tacggtttat aacgaattga 2341 caaaggtcaa atatgttact gaaggaatgc gaaaaccagc atttctttca ggtgaacaga 2401 agaaagccat tgttgattta ctcttcaaaa caaatcgaaa agtaaccgtt aagcaattaa 2461 aagaagatta tttcaaaaaa atagaatgtt ttgatagtgt tgaaatttca ggagttgaag 2521 atagatttaa tgcttcatta ggtacctacc atgatttgct aaaaattatt aaagataaag 2581 attttttgga taatgaagaa aatgaagata tcttagagga tattgtttta acattgacct 2641 tatttgaaga tagggagatg attgaggaaa gacttaaaac atatgctcac ctctttgatg 2701 ataaggtgat gaaacagctt aaacgtcgcc gttatactgg ttggggagct ttgtctcgaa 2761 aattgattaa tggtattagg gataagcaat ctggcaaaac aatattagat tttttgaaat 2821 cagatggttt tgccaatcgc aattttatgg cgttaataca cgacgactct ttgacattta 2881 aagaagacat tcaaaaagca caagtgtctg gacaaggcga tagtttacat gaacatattg 2941 caaatttagc tggtagccct gctattaaaa aaggtatttt acagactgta aaagttgttg 3001 atgaattggt caaagtaatg gggcggcata agccagaaaa tatcgttatt gaaatggcac 3061 gtgaaaatca gacaactcaa aagggccaga aaaattcgcg agagcgtatg aaacgaatcg 3121 aagaaggtat caaagaatta ggaagtcaga ttcttaaaga gcatcctgtt gaaaatactc 3181 aattgcaaaa tgaaaagctc tatctctatt atctccaaaa tggaagagac atgtatgtgg 3241 accaagaatt agatattaat cgtttaagtg attatgatgt cgatcacatt gttccacaaa 3301 gtttccttaa agacgattca atagacaata aggtcttaac gcgttctgat aaaaatcgtg 3361 gtaaatcgga taacgttcca agtgaagaag tagtcaaaaa gatgaaaaac tattggagac 3421 aacttctaaa cgccaagtta atcactcaac gtaagtttga taatttaacg aaagctgaac 3481 gtggaggttt gagtgaactt gataaagctg gttttatcaa acgccaattg gttgaaactc 3541 gcgcaatcac taagcatgtg gcacaaattt tggatagtcg catgaatact aaatacgatg 3601 aaaatgataa acttattcga gaggttaaag tgattacctt aaaatctaaa ttagtttctg 3661 acttccgaaa agatttccaa ttctataaag tacgtgagat taacaattac catcatgccc 3721 atgatgcgta tctaaatgcc gtcgttggaa ctgctttgat taagaaatat ccaaaacttg 3781 aatcggagtt tgtctatggt gattataaag tttatgatgt tcgtaaaatg attgctaagt 3841 ctgagcaaga aataggcaaa gcaaccgcaa aatatttctt ttactctaat atcatgaact 3901 tcttcaaaac agaaattaca cttgcaaatg gagagattcg caaacgccct ctaatcgaaa 3961 ctaatgggga aactggagaa attgtctggg ataaagggcg agattttgcc acagtgcgca 4021 aagtattgtc catgccccaa gtcaatattg tcaagaaaac agaagtacag acaggcggat 4081 tctccaagga gtcaatttta ccaaaaagaa attcggacaa gcttattgct cgtaaaaaag 4141 actgggatcc aaaaaaatat ggtggttttg atagtccaac ggtagcttat tcagtcctag 4201 ttgttgctaa ggtggaaaaa gggaaatcga agaagttaaa atccgttaaa gagttactag 4261 ggatcacaat tatggaaaga agttcctttg aaaaaaatcc gattgacttt ttagaagcta 4321 aaggatataa ggaagttaaa aaagacttaa tcattaaact acctaaatat agtctttttg 4381 agttagaaaa cggtcgtaaa cggatgctgg ctagtgccgg agaattacaa aaaggaaatg 4441 agctggctct gccaagcaaa tatgtgaatt ttttatattt agctagtcat tatgaaaagt 4501 tgaagggtag tccagaagat aacgaacaaa aacaattgtt tgtggagcag cataagcatt 4561 atttagatga gattattgag caaatcagtg aattttctaa gcgtgttatt ttagcagatg 4621 ccaatttaga taaagttctt agtgcatata acaaacatag agacaaacca atacgtgaac 4681 aagcagaaaa tattattcat ttatttacgt tgacgaatct tggagctccc gctgctttta 4741 aatattttga tacaacaatt gatcgtaaac gatatacgtc tacaaaagaa gttttagatg 4801 ccactcttat ccatcaatcc atcactggtc tttatgaaac acgcattgat ttgagtcagc 4861 taggaggtga ctctggtggt tctactaatc tgtcagatat tattgaaaag gagaccggta 4921 agcaactggt tatccaggaa tccatcctca tgctcccaga ggaggtggaa gaagtcattg 4981 ggaacaagcc ggaaagcgat atactcgtgc acaccgccta cgacgagagc accgacgaga 5041 atgtcatgct tctgactagc gacgcccctg aatacaagcc ttgggctctg gtcatacagg 5101 atagcaacgg tgagaacaag attaagatgc tctctggtgg ttctcccaag aagaagagga 5161 aagtctaata attgattaat acctaggctg ctaaacaaag cccgaaagga agctgagttg 5221 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg 5281 aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta 5341 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 5401 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 5461 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc 5521 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat 5581 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 5641 aaactggaac aacactcaac catatctcgg tctattcttt tgatttataa gggattttgc 5701 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta 5761 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc 5821 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgaatt aattcttaga 5881 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat 5941 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga 6001 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta 6061 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat 6121 ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat 6181 tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct 6241 gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca 6301 accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt 6361 ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag 6421 gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc 6481 tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact 6541 ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat 6601 cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctag 6661 agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag 6721 cagacagttt tattgttcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 6781 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 6841 tgctgattgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 6901 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc 6961 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 7021 ctcgctctgc taatcatgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 7081 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 7141 tcgtgcacac agccaagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 7201 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 7261 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 7321 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 7381 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 7441 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt 7501 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag 7561 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc 7621 ggtatttcac accgcaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa 7681 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc 7741 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc 7801 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc 7861 gaggcagctg cggtaaagct cataagcgtg gtcgtgaagc gattcacaga tgtctgcctg 7921 ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa 7981 gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg 8041 atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt 8101 tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg 8161 gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga 8221 tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt 8281 gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca 8341 tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat 8401 cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga 8461 caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc 8521 gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggagt gcaagattcc 8581 gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa 8641 aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat 8701 aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc 8761 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc 8821 gttgcgctca ctgccagctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 8881 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca 8941 ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca 9001 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg 9061 ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa 9121 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa 9181 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg 9241 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat 9301 atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca 9361 gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt 9421 catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg 9481 gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa 9541 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga 9601 cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt 9661 taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa 9721 tcagcaacaa ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct 9781 ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcatggttca 9841 ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta 9901 ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc 9961 gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc 10021 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat 10081 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc 10141 acgccgaaac aagcgctcat gagcccaaag tggcgagccc gatcttcccc atcggtgatg 10201 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt 10261 ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat 10321 tgtgagcgga taacaattcc cctctagaaa taattttgtt taactttaag aagga //