LOCUS Exported 3006 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Constitutive E. coli mcherry Expression. ACCESSION . VERSION . KEYWORDS pCDF-mcherry1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3006) TITLE SUPERSEDE JOURNAL Unpublished REFERENCE 2 (bases 1 to 3006) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3006 /organism="synthetic DNA construct" /mol_type="other DNA" RBS 200..211 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" CDS 218..928 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(363..381) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 631..650 /label=mCherry-F /note="mCherry, forward primer" CDS complement(1018..1809) /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRTDQLEEFVHYVKGEITKVVGK" promoter complement(1810..1901) /gene="bla" /label=AmpR promoter rep_origin complement(1949..2687) /direction=LEFT /label=CloDF13 ori /note="Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins." ORIGIN 1 cgcacgtctc catcgcttgc ccaagttgtg aagcacagct aacaccacgt cgtccctatc 61 tgctgcccta ggtctatgag tggttgctgg ataactttac gggcatgcat aaggctcgta 121 taatatattc agggagacca caacggtttc cctctacaaa taattttgtt taactttgat 181 cgcatggttg ctactagaga aagaggagaa atactagatg gtgagcaagg gcgaggagga 241 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa 301 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac 361 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc 421 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt 481 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg 541 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa 601 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg 661 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca 721 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc 781 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc 841 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac 901 cggcggtatg gacgagctgt acaagtaata aggacgagcc tcagaccatt taaatcatac 961 ctgaccgtca tcgtggccgg atcttgcggc ccctcggctt gaacgaattg ttagacatta 1021 tttgccgact accttggtga tctcgccttt cacgtagtgg acaaattctt ccaactgatc 1081 tgtgcgcgag gccaagcgat cttcttcttg tccaagataa gcctgtctag cttcaagtat 1141 gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg 1201 cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg 1261 ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc 1321 aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc 1381 aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg 1441 ctcgaagata cctgcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt 1501 agctggataa cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg 1561 gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga tcaaagctcg 1621 ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg 1681 cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag 1741 atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc 1801 ttccctcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 1861 gagcggatac atatttgaat gtatttagaa aaataaacaa atagctagct cactcggtcg 1921 ctacgctccg ggcgtgagac tgcggcgggc gctgcggaca catacaaagt tacccacaga 1981 ttccgtggat aagcagggga ctaacatgtg aggcaaaaca gcagggccgc gccggtggcg 2041 tttttccata ggctccgccc tcctgccaga gttcacataa acagacgctt ttccggtgca 2101 tctgtgggag ccgtgaggct caaccatgaa tctgacagta cgggcgaaac ccgacaggac 2161 ttaaagatcc ccaccgtttc cggcgggtcg ctccctcttg cgctctcctg ttccgaccct 2221 gccgtttacc ggatacctgt tccgcctttc tcccttacgg gaagtgtggc gctttctcat 2281 agctcacaca ctggtatctc ggctcggtgt aggtcgttcg ctccaagctg ggctgtaagc 2341 aagaactccc cgttcagccc gactgctgcg ccttatccgg taactgttca cttgagtcca 2401 acccggaaaa gcacggtaaa acgccactgg cagcagccat tggtaactgg gagttcgcag 2461 aggatttgtt tagctaaaca cgcggttgct cttgaagtgt gcgccaaagt ccggctacac 2521 tggaaggaca gatttggttg ctgtgctctg cgaaagccag ttaccacggt taagcagttc 2581 cccaactgac ttaacgttcg atcaaaccac ctccccaggt ggttttttcg tttacagggc 2641 aaaagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctactgaac 2701 cgctctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc agccccatac 2761 gatataagtt gtaattctca tgttagtcat gccccgcgcc caccggaagg agctgactgg 2821 gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac 2881 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 2941 ttaacagtcc agttacgctg gagtcgcctc cggtccgcaa aaaaccccgc ttcggcgggg 3001 tttttt //