LOCUS Exported 4900 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION PCR tagging module with yeast codon optimized mTurquoise2. ACCESSION . VERSION . KEYWORDS pSJ1458 (pFA6-link-mTurquoise2-CaURA3MX) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4900) AUTHORS Burns S, Avena JS, Unruh JR, Yu Z, Smith SE, Slaughter BD, Winey M, Jaspersen SL TITLE Structured illumination with particle averaging reveals novel roles for yeast centrosome components during duplication. JOURNAL Elife. 2015 Sep 15;4. doi: 10.7554/eLife.08586. PUBMED 26371506 REFERENCE 2 (bases 1 to 4900) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4900 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 68..86 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(124..146) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 246..265 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" promoter 346..364 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 346..363 /label=SP6 /note="SP6 promoter, forward primer" terminator 1171..1358 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" primer_bind complement(1226..1250) /label=pHybLex-R /note="ADH terminator, reverse primer" promoter 1433..1776 /label=TEF promoter /note="Ashbya gossypii TEF promoter" terminator 2595..2792 /label=TEF terminator /note="Ashbya gossypii TEF terminator" primer_bind complement(2899..2918) /label=T7 /note="T7 promoter, forward primer" promoter complement(2900..2918) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3005..3022) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3176..3764) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3256..3275) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3935..4795) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4558..4577 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(4796..4900) /gene="bla" /label=AmpR promoter ORIGIN 1 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 61 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga 121 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg 181 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa 241 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atggacatat tgtcgttaga 301 acgcggctac aattaataca taaccttatg tatcatacac atacgattta ggtgacacta 361 tagaacgcgg ccgccagctg aagcttcgta cgctgcaggt cgacggatcg gtgacggtgc 421 tggtttaatt aacatgtcta aaggtgaaga attgtttact ggtgttgttc caattttggt 481 tgaattggat ggtgatgtta atggtcataa attttctgtt tctggtgaag gtgaaggtga 541 tgctacttat ggtaaattga ctttgaaatt tatttgtact actggtaaat tgccagttcc 601 atggccaact ttggttacta ctttgtcttg gggtgttcaa tgttttgcta gatatccaga 661 tcatatgaaa caacatgatt tttttaaatc tgctatgcca gaaggttatg ttcaagaaag 721 aactattttt tttaaagatg atggtaatta taaaactaga gctgaagtta aatttgaagg 781 tgatactttg gttaatagaa ttgaattgaa aggtattgat tttaaagaag atggtaatat 841 tttgggtcat aaattggaat ataattattt ttctgataat gtttatatta ctgctgataa 901 acaaaaaaat ggtattaaag ctaattttaa aattagacat aatattgaag atggtggtgt 961 tcaattggct gatcattatc aacaaaatac tccaattggt gatggtccag ttttgttgcc 1021 agataatcat tatttgtcta ctcaatctgc tttgtctaaa gatccaaatg aaaaaagaga 1081 tcatatggtt ttgttggaat ttgttactgc tgctggtatt actttgggta tggatgaatt 1141 gtataaataa ggcgcgccac ttctaaataa gcgaatttct tatgatttat gatttttatt 1201 attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta 1261 aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta 1321 tagtatgagg tcgctcttat tgaccacacc tctaccggca gatccgctag ggataacagg 1381 gtaatataga tctgtttagc ttgcctcgtc cccgccgggt cacccggcca gcgacatgga 1441 ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg 1501 tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca tacattttga 1561 tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg cggacctgcg agcagggaaa 1621 cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa 1681 aggttaggat ttgccactga ggttcttctt tcatatactt ccttttaaaa tcttgctagg 1741 atacagttct cacatcacat ccgaacataa acaaccatga cagtcaacac taagacctat 1801 agtgagagag cagaaactca tgcctcacca gtagcacaac gattatttcg attaatggaa 1861 ctgaagaaaa ccaatttatg tgcatcaatt gatgttgata ccactaagga attccttgaa 1921 ttaattgata aattgggtcc ttatgtatgc ttaatcaaga ctcatattga tataatcaat 1981 gatttttcct atgaatccac tattgaacca ttattagaac tttcacgtaa acatcaattt 2041 atgatttttg aagatagaaa atttgctgat attggtaata ccgtgaagaa acaatatatt 2101 ggtggagttt ataaaattag tagttgggca gatattacta atgctcatgg tgtcactggg 2161 aatggagtag ttgaaggatt aaaacaggga gctaaagaaa ccaccaccaa ccaagagcca 2221 agagggttat tgatgttagc tgaattatca tcagtgggat cattagcata tggagaatat 2281 tctcaaaaaa ctgttgaaat tgctaaatcc gataaggaat ttgttattgg atttattgcc 2341 caacgtgata tgggtggaca agaagaagga tttgattggc ttattatgac acctggagtt 2401 ggattagatg ataaaggtga tggattagga caacaatata gaactgttga tgaagttgtt 2461 agcactggaa ctgatattat cattgttggt agaggattgt ttggtaaagg aagagatcca 2521 gatattgaag gtaaaaggta tagagatgct ggttggaatg cttatttgaa aaagactggc 2581 caattataat cagtactgac aataaaaaga ttcttgtttt caagaacttg tcatttgtat 2641 agttttttta tattgtagtt gttctatttt aatcaaatgt tagcgtgatt tatatttttt 2701 ttcgcctcga catcatctgc ccagatgcga agttaagtgc gcagaaagta atatcatgcg 2761 tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat tcgatactaa cgccgccatc 2821 cagtgtcgaa aacgagctcg aattcatcga tgatatcaga tccactagtg gcctatgcgg 2881 ccgcggatct gccggtctcc ctatagtgag tcgtattaat ttcgataagc caggttaacc 2941 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 3001 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 3061 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 3121 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 3181 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 3241 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 3301 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 3361 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 3421 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 3481 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 3541 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 3601 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 3661 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 3721 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 3781 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 3841 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 3901 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 3961 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 4021 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 4081 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 4141 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 4201 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 4261 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 4321 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 4381 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 4441 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 4501 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 4561 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 4621 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 4681 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 4741 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 4801 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 4861 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg //