LOCUS Exported 10360 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION DSG-2 tension sensor, using TSmod inserted in human DSG-2. ACCESSION . VERSION . KEYWORDS pcdna DSG-2 TS SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10360) AUTHORS Baddam SR, Arsenovic PT, Narayanan V, Duggan NR, Mayer CR, Newman ST, Abutaleb DA, Mohan A, Kowalczyk AP, Conway DE TITLE The Desmosomal Cadherin Desmoglein-2 Experiences Mechanical Tension as Demonstrated by a FRET-Based Tension Biosensor Expressed in Living Cells. JOURNAL Cells. 2018 Jun 26;7(7). pii: cells7070066. doi: 10.3390/cells7070066. PUBMED 29949915 REFERENCE 2 (bases 1 to 10360) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10360 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 169..548 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 549..752 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 703..723 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 797..816 /label=T7 /note="T7 promoter, forward primer" promoter 797..815 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 3139..3846 /codon_start=1 /product="monomeric teal (cyan) fluorescent protein (Ai et al., 2006)" /label=mTFP1 /note="mammalian codon-optimized" /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA VARNSTDGMDELYK" CDS 3967..4683 /codon_start=1 /product="Venus YFP with monomerizing A206K mutation (Nagai et al., 2002; Kremers et al., 2006)" /label=mVenus /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(4012..4033) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(4273..4292) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 4620..4641 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(5885..5902) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 5891..6115 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 6161..6589 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6248..6267) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6458..6479 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(6598..6618) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 6603..6932 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 6783..6918 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6845..6864 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 6999..7793 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(7053..7072) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 7663..7682 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 7967..8088 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8004..8023) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8058..8077 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8137..8153) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8137..8153) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8150..8172) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8161..8177 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8185..8215) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8230..8251 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8368..8385) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8539..9127) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8619..8638) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9298..10158) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9921..9940 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10159..10263) /gene="bla" /label=AmpR promoter primer_bind complement(10338..10357) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca 61 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg 121 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat 181 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt 241 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 301 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 361 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 421 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 481 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 541 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 601 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 661 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 721 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt 781 actggcttat cgaaattaat acgactcact atagggagac ccaagctggc tagcgtttaa 841 acttaagctt ggtaccgagc tcggatccac tagtccagtg tggtggaatt ctgcagatat 901 ccagcacagt ggcggccgct cgagggtgcg atggcgcgga gcccgggacg cgcgtacgcc 961 ctgctgcttc tcctgatctg ctttaacgtt ggaagtggac ttcacttaca ggtcttaagc 1021 acaagaaatg aaaataagct gcttcctaaa catcctcatt tagtgcggca aaagcgcgcc 1081 tggatcaccg cccccgtggc tcttcgggag ggagaggatc tgtccaagaa gaatccaatt 1141 gccaagatac attctgatct tgcagaagaa agaggactca aaattactta caaatacact 1201 ggaaaaggga ttacagagcc accttttggt atatttgtct ttaacaaaga tactggagaa 1261 ctgaatgtta ccagcattct tgatcgagaa gaaacaccat tttttctgct aacaggttac 1321 gctttggatg caagaggaaa caatgtagag aaacccttag agctacgcat taaggttctt 1381 gatatcaatg acaacgaacc agtgttcaca caggatgtct ttgttgggtc tgttgaagag 1441 ttgagtgcag cacatactct tgtgatgaaa atcaatgcaa cagatgcaga tgagcccaat 1501 accctgaatt cgaaaatttc ctatagaatc gtatctctgg agcctgctta tcctccagtg 1561 ttctacctaa ataaagatac aggagagatt tatacaacca gtgttacctt ggacagagag 1621 gaacacagca gctacacttt gacagtagaa gcaagagatg gcaatggaga agttacagac 1681 aaacctgtaa aacaagctca agttcagatt cgtattttgg atgtcaatga caatatacct 1741 gtagtagaaa ataaagtgct tgaagggatg gttgaagaaa atcaagtcaa tgtagaagtt 1801 acgcgcataa aagtgttcga tgcagatgaa ataggttctg ataattggct ggcaaatttt 1861 acatttgcat caggaaatga aggaggttat ttccacatag aaacagatgc tcaaactaac 1921 gaaggaattg tgacccttat taaggaagta gattatgaag aaatgaagaa tcttgacttc 1981 agtgttattg tcgctaataa agcagctttt cacaagtcga ttaggagtaa atacaagcct 2041 acacccattc ccatcaaggt caaagtgaaa aatgtgaaag aaggcattca ttttaaaagc 2101 agcgtcatct caatttatgt tagcgagagc atggatagat caagcaaagg ccaaataatt 2161 ggaaattttc aagcttttga tgaggacact ggactaccag cccatgcaag atatgtaaaa 2221 ttagaagata gagataattg gatctctgtg gattctgtca catctgaaat taaacttgca 2281 aaacttcctg attttgaatc tagatatgtt caaaatggca catacactgt aaagattgtg 2341 gccatatcag aagattatcc tagaaaaacc atcactggca cagtccttat caatgttgaa 2401 gacatcaacg acaactgtcc cacactgata gagcctgtgc agacaatctg tcacgatgca 2461 gagtatgtga atgttactgc agaggacctg gatggacacc caaacagtgg ccctttcagt 2521 ttctccgtca ttgacaaacc acctggcatg gcagaaaaat ggaaaatagc acgccaagaa 2581 agtaccagtg tgctgctgca acaaagtgag aaaaagcttg ggagaagtga aattcagttc 2641 ctgatttcag acaatcaggg ttttagttgt cctgaaaagc aggtccttac actcacagtt 2701 tgtgagtgtc tgcatggcag cggctgcagg gaagcacagc atgactccta tgtgggcctg 2761 ggacccgcag caattgcgct catgattttg gcctttctgc tcctgctatt ggtaccactt 2821 ttactgctga tgtgccattg cggaaagggc gccaaaggct ttacccccat acctggcacc 2881 atagagatgc tgcatccttg gaataatgaa ggagcaccac ctgaagacaa ggtggtgcca 2941 tcatttctgc cagtggatca agggggcagt ctagtaggaa gaaatggagt aggaggtatg 3001 gccaaggaag ccacgatgaa aggaagtagc tctgcttcca ttgtcaaagg gcaacatgag 3061 atgtccgaga tggatggaag gtgggaagaa cacagaagcc tgctttctgg tagagctacc 3121 cagtttacag gggtcgagat ggtgagcaag ggcgaggaga ccacaatggg cgtaatcaag 3181 cccgacatga agatcaagct gaagatggag ggcaacgtga atggccacgc cttcgtgatc 3241 gagggcgagg gcgagggcaa gccctacgac ggcaccaaca ccatcaacct ggaggtgaag 3301 gagggagccc ccctgccctt ctcctacgac attctgacca ccgcgttcgc ctacggcaac 3361 agggccttca ccaagtaccc cgacgacatc cccaactact tcaagcagtc cttccccgag 3421 ggctactctt gggagcgcac catgaccttc gaggacaagg gcatcgtgaa ggtgaagtcc 3481 gacatctcca tggaggagga ctccttcatc tacgagatac acctcaaggg cgagaacttc 3541 ccccccaacg gccccgtgat gcagaagaag accaccggct gggacgcctc caccgagagg 3601 atgtacgtgc gcgacggcgt gctgaagggc gacgtcaagc acaagctgct gctggagggc 3661 ggcggccacc accgcgttga cttcaagacc atctacaggg ccaagaaggc ggtgaagctg 3721 cccgactatc actttgtgga ccaccgcatc gagatcctga accacgacaa ggactacaac 3781 aaggtgaccg tttacgagag cgccgtggcc cgcaactcca ccgacggcat ggacgagctg 3841 tacaaggggc caggtggtgc agggccaggt ggtgcagggc caggtggtgc agggccaggt 3901 ggtgcagggc ccggtggtgc aggtccaggt ggtgcaggtc caggtggtgc aggtccaggt 3961 ggtgctatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag 4021 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc 4081 acctacggca agctgaccct gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg 4141 cccaccctcg tgaccaccct gggctacggc ctgcagtgct tcgcccgcta ccccgaccac 4201 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc 4261 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac 4321 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg 4381 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcaccgc cgacaagcag 4441 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag 4501 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac 4561 aaccactacc tgagctacca gtccaaactg agcaaagacc ccaacgagaa gcgcgatcac 4621 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac 4681 aaggcggccg cggccacagg cgctatcatg accactgaaa ccacgaagac cgcaagggcc 4741 acaggggctt ccagagacat ggccggagct caggcagctg ctgttgcact gaacgaagaa 4801 ttcttaagaa attatttcac tgataaagcg gcctcttaca ctgaggaaga tgaaaatcac 4861 acagccaaag attgccttct ggtttattct caggaagaaa ctgaatcgct gaatgcttct 4921 attggttgtt gcagttttat tgaaggagag ctagatgacc gcttcttaga tgatttggga 4981 cttaaattca agacactagc tgaagtttgc ctgggtcaaa aaatagatat aaataaggaa 5041 attgagcaga gacaaaaacc tgccacagaa acaagtatga acacagcttc acattcactc 5101 tgtgagcaaa ctatggttaa ttcagagaat acctactcct ctggcagtag cttcccagtt 5161 ccaaaatctt tgcaagaagc caatgcagag aaagtaactc aggaaatagt cactgaaaga 5221 tctgtgtctt ctaggcaggc gcaaaaggta gctacacctc ttcctgaccc aatggcttct 5281 agaaatgtga tagcaacaga aacttcctat gtcacagggt ccactatgcc accaaccact 5341 gtgatcctgg gtcctagcca gccacagagc cttattgtga cagagagggt gtatgctcca 5401 gcttctacct tggtagatca gccttatgct aatgaaggta cagttgtggt cactgaaaga 5461 gtaatacagc ctcatggggg tggatcgaat cctctggaag gcactcagca tcttcaagat 5521 gtaccttacg tcatggtgag ggaaagagag agcttccttg cccccagctc aggtgtgcag 5581 cctactctgg ccatgcctaa tatagcagta ggacagaatg tgacagtgac agaaagagtt 5641 ctagcacctg cttccactct gcaatccagt taccagattc ccactgaaaa ttctatgacg 5701 gctaggaaca ccacggtgtc tggagctgga gtccctggcc ctctgccaga ttttggttta 5761 gaggaatctg gtcattctaa ttctaccata accacatctt ccaccagagt taccaagcat 5821 agcactgtac agcattctta ctcctaactc gagtctagag ggcccgttta aacccgctga 5881 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct 5941 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca 6001 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag 6061 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct 6121 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca 6181 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 6241 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 6301 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 6361 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 6421 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 6481 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 6541 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga 6601 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 6661 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 6721 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 6781 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 6841 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag 6901 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt 6961 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 7021 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 7081 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 7141 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat 7201 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 7261 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 7321 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 7381 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 7441 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 7501 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc 7561 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 7621 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 7681 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 7741 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac 7801 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc 7861 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 7921 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc 7981 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 8041 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat 8101 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 8161 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 8221 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 8281 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 8341 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 8401 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 8461 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 8521 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 8581 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 8641 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 8701 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 8761 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 8821 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 8881 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 8941 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 9001 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 9061 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 9121 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 9181 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 9241 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 9301 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 9361 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 9421 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 9481 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 9541 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 9601 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 9661 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 9721 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 9781 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 9841 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 9901 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 9961 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 10021 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 10081 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 10141 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 10201 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 10261 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc 10321 ctatggtgca ctctcagtac aatctgctct gatgccgcat //