LOCUS       Exported                9571 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    TetO-FUW-oct4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9571)
  AUTHORS   Brambrink T, Foreman R, Welstead GG, Lengner CJ, Wernig M, Suh H, 
            Jaenisch R
  TITLE     Sequential expression of pluripotency markers during direct 
            reprogramming of mouse somatic cells.
  JOURNAL   Cell Stem Cell. 2008 Feb 7. 2(2):151-9.
  PUBMED    18371436
REFERENCE   2  (bases 1 to 9571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9571
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     771..791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1679..1912
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2097..2141
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2439..2556
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2613..2631
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2655..2673
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2697..2715
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2739..2757
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2790..2828
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2825..2849
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4262..4850
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4315..4335)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4733..4744)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4853..4869)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4854..4870)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5375..5555
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5581..5598)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5587..5811
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5857..6285
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5944..5963)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6154..6175
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6294..6314)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6299..6628
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6479..6614
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6541..6560
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6676..6723
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6742..7116
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7246..7367
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7283..7302)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7337..7356
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7416..7432)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7416..7432)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7429..7451)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7440..7456
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7464..7494)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7509..7530
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7647..7664)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7818..8406)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7898..7917)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8577..9437)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9200..9219
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9438..9542)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     1561 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1621 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1681 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1741 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1801 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1861 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1921 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1981 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     2041 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     2101 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     2161 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     2221 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     2281 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     2341 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
     2401 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     2461 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2521 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     2581 gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     2701 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     2761 gtgaaagtcg agctcggtac ccgggtcgag gtaggcgtgt acggtgggag gcctatataa
     2821 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2881 tccatagaag acaccgggac cgatccagcc tccgcggccc cgaattcgcc ctttgagccg
     2941 tctttccacc aggcccccgg ctcggggtgc ccaccttccc catggctgga cacctggctt
     3001 cagacttcgc cttctcaccc ccaccaggtg ggggtgatgg gtcagcaggg ctggagccgg
     3061 gctgggtgga tcctcgaacc tggctaagct tccaagggcc tccaggtggg cctggaatcg
     3121 gaccaggctc agaggtattg gggatctccc catgtccgcc cgcatacgag ttctgcggag
     3181 ggatggcata ctgtggacct caggttggac tgggcctagt cccccaagtt ggcgtggaga
     3241 ctttgcagcc tgagggccag gcaggagcac gagtggaaag caactcagag ggaacctcct
     3301 ctgagccctg tgccgaccgc cccaatgccg tgaagttgga gaaggtggaa ccaactcccg
     3361 aggagtccca ggacatgaaa gccctgcaga aggagctaga acagtttgcc aagctgctga
     3421 agcagaagag gatcaccttg gggtacaccc aggccgacgt ggggctcacc ctgggcgttc
     3481 tctttggaaa ggtgttcagc cagaccacca tctgtcgctt cgaggccttg cagctcagcc
     3541 ttaagaacat gtgtaagctg cggcccctgc tggagaagtg ggtggaggaa gccgacaaca
     3601 atgagaacct tcaggagata tgcaaatcgg agaccctggt gcaggcccgg aagagaaagc
     3661 gaactagcat tgagaaccgt gtgaggtgga gtctggagac catgtttctg aagtgcccga
     3721 agccctccct acagcagatc actcacatcg ccaatcagct tgggctagag aaggatgtgg
     3781 ttcgagtatg gttctgtaac cggcgccaga agggcaaaag atcaagtatt gagtattccc
     3841 aacgagaaga gtatgaggct acagggacac ctttcccagg gggggctgta tcctttcctc
     3901 tgcccccagg tccccacttt ggcaccccag gctatggaag cccccacttc accacactct
     3961 actcagtccc ttttcctgag ggcgaggcct ttccctctgt tcccgtcact gctctgggct
     4021 ctcccatgca ttcaaactga ggcaccagcc ctccctgggg atgctgtgag ccaaggcaag
     4081 ggaggtagac aagagaacct ggagctttgg ggttaaattc ttttactgag gagggattaa
     4141 aagcacaaca ggggtggggg gtgggatggg gaaagaagct cagtgatgct gttgatcagg
     4201 agcctggcct gtctgtcact cattttgttc taagggcgaa ttcgatatca agcttatcga
     4261 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     4321 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     4381 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     4441 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     4501 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     4561 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     4621 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     4681 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     4741 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     4801 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     4861 tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     4921 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     4981 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     5041 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     5101 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
     5161 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
     5221 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
     5281 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
     5341 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
     5401 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     5461 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     5521 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag
     5581 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     5641 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     5701 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     5761 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     5821 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     5881 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     5941 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6001 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6061 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6121 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6181 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6241 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     6301 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6361 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     6421 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     6481 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     6541 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     6601 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     6661 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     6721 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
     6781 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
     6841 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
     6901 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
     6961 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
     7021 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
     7081 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
     7141 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     7201 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     7261 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7321 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     7381 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7441 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7501 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7561 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7621 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7681 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7741 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7801 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7861 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7921 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7981 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     8041 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8101 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8161 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8221 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     8281 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8341 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8401 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     8461 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     8521 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     8581 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8641 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8701 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8761 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8821 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8881 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8941 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9001 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9061 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9121 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9181 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9241 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9301 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9361 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9421 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     9481 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     9541 cgcacatttc cccgaaaagt gccacctgac g
//
