LOCUS       Exported                2843 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Plasmid encodes a synthetic CRISPR containing 7 copies of a single 
            spacer, which targets a region (gene 24) in the genome of 
            bacteriophage DMS3.
ACCESSION   .
VERSION     .
KEYWORDS    pCRISPR_DMS3g24
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Chowdhury S, Carter J, Rollins MF, Golden SM, Jackson RN, Hoffmann 
            C, Nosaka L, Bondy-Denomy J, Maxwell KL, Davidson AR, Fischer ER, 
            Lander GC, Wiedenheft B
  TITLE     Structure Reveals Mechanisms of Viral Suppressors that Intercept a 
            CRISPR RNA-Guided Surveillance Complex.
  JOURNAL   Cell. 2017 Mar 23;169(1):47-57.e11. doi: 10.1016/j.cell.2017.03.012.
  PUBMED    28340349
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        397..415
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      864..910
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1049..1066)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1159..1747)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1239..1258)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1921..2715)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2716..2820)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatg agctcgcgac tcctgcatta ggaaattaat acgactcact atagggttca
      421 ctgccgtgta ggcagctaag aaattcacgg cgggcttgat gtccgcgtct acctggttca
      481 ctgccgtgta ggcagctaag aaattcacgg cgggcttgat gtccgcgtct acctggttca
      541 ctgccgtgta ggcagctaag aaattcacgg cgggcttgat gtccgcgtct acctggttca
      601 ctgccgtgta ggcagctaag aaattcacgg cgggcttgat gtccgcgtct acctggttca
      661 ctgccgtgta ggcagctaag aaattcacgg cgggcttgat gtccgcgtct acctggttca
      721 ctgccgtgta ggcagctaag aaattcacgg cgggcttgat gtccgcgtct acctggttca
      781 ctgccgtgta ggcagctaag aaattcacgg cgggcttgat gtccgcgtct acctggttca
      841 ctgccgtgta ggcagctaag aaatagcata accccttggg gcctctaaac gggtcttgag
      901 gggttttttg ggtaccctcg agtctggtga attcctgggc ctcatgggcc ttcctttcac
      961 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaacatggt catagctgtt
     1021 tccttgcgta ttgggcgctc tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1081 gggtaaagcc tggggtgcct aatgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1141 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1201 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1261 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1321 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     1381 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1441 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     1501 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     1561 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     1621 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     1681 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     1741 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     1801 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     1861 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     1921 ttagaaaaat tcatccagca gacgataaaa cgcaatacgc tggctatccg gtgccgcaat
     1981 gccatacagc accagaaaac gatccgccca ttcgccgccc agttcttccg caatatcacg
     2041 ggtggccagc gcaatatcct gataacgatc cgccacgccc agacggccgc aatcaataaa
     2101 gccgctaaaa cggccatttt ccaccataat gttcggcagg cacgcatcac catgggtcac
     2161 caccagatct tcgccatccg gcatgctcgc tttcagacgc gcaaacagct ctgccggtgc
     2221 caggccctga tgttcttcat ccagatcatc ctgatccacc aggcccgctt ccatacgggt
     2281 acgcgcacgt tcaatacgat gtttcgcctg atgatcaaac ggacaggtcg ccgggtccag
     2341 ggtatgcaga cgacgcatgg catccgccat aatgctcact ttttctgccg gcgccagatg
     2401 gctagacagc agatcctgac ccggcacttc gcccagcagc agccaatcac ggcccgcttc
     2461 ggtcaccaca tccagcaccg ccgcacacgg aacaccggtg gtggccagcc agctcagacg
     2521 cgccgcttca tcctgcagct cgttcagcgc accgctcaga tcggttttca caaacagcac
     2581 cggacgaccc tgcgcgctca gacgaaacac cgccgcatca gagcagccaa tggtctgctg
     2641 cgcccaatca tagccaaaca gacgttccac ccacgctgcc gggctacccg catgcaggcc
     2701 atcctgttca atcatactct tcctttttca atattattga agcatttatc agggttattg
     2761 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     2821 cacatttccc cgaaaagtgc cac
//
