LOCUS       Exported                 969 bp ds-DNA     linear   SYN 12-JUN-2021
DEFINITION  HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of 
            the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 
            2009 ), expressed via the TEF1 promoter. HO locus integration..
ACCESSION   .
VERSION     .
KEYWORDS    pTEF:ATP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Papagiannakis A, Niebel B, Wit EC, Heinemann M
  TITLE     Autonomous Metabolic Oscillations Robustly Gate the Early and Late 
            Cell Cycle.
  JOURNAL   Mol Cell. 2017 Jan 19;65(2):285-295. doi: 
            10.1016/j.molcel.2016.11.018. Epub 2016 Dec 15.
  PUBMED    27989441
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      183..372
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
ORIGIN
        1 aatgttgttt gcatttatga tccgttatat tttgatctaa tgtagagttg cacgtagttc
       61 ttactggcaa agaaatcgat gcataccaaa aaagaataaa ggtgatattt gatctttacc
      121 gtttagttcc aacgtaaaat tgtgcctttg gacttaaaat ggcgtcgtac gctgagctgg
      181 atcttcgagc gtcccaaaac cttctcaagc aaggttttca gtataatgtt acatgcgtac
      241 acgcgtctgt acagaaaaaa aagaaaaatt tgaaatataa ataacgttct taatactaac
      301 ataactataa aaaaataaat agggacctag acttcaggtt gtctaactcc ttccttttcg
      361 gttagagcgg atcttagcta gccgcggtac caagcttact cgagtcattc aatgttatgt
      421 ctaattttga agttggcctt gataccattt ttttgtttgt cagcagtgat gtagacgttg
      481 tgagagttat aattgtattc taacttatga cctaaaatgt taccatcttc tttaaaatca
      541 atacctttca attcaattct attgaccaaa gtgtcacctt caaattttac ttcagctctg
      601 gtcttgtaat taccatcgtc cttaaagaaa atagttcttt cttggacata accttcaggc
      661 attgcagact taaaaaagtc atgttgcttc atgtggtcag gatatctagc gaagcattgt
      721 aaaccgtaac ccaatgttgt aactaatgta ggccaaggaa caggtaactt accggtagtg
      781 catatcaatt tcaaagtcaa tttaccgtat gttgcgtcac cttcaccttc accactaact
      841 gaaaacttat gaccattgac gtcaccgtct aattctacta aaatagggac gacacctgtg
      901 aataattctt cacctttact aaccatacca ccagaaccac ccttgtataa ttcatccata
      961 cccaaagta
//
